Development of an Interception Glove Sampler for Skin Exposures to Aromatic Isocyanates
Objectives: Skin is an important exposure route for isocyanate chemicals and contributes to systemic sensitization. Methods for assessing skin exposure are currently limited and generally rely upon removal (e.g. tape-strip) techniques prone to underestimation. The aim of this study is to (i) develop...
Gespeichert in:
| Veröffentlicht in: | The Annals of occupational hygiene 2016-11, Vol.60 (9), p.1092-1103 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1103 |
|---|---|
| container_issue | 9 |
| container_start_page | 1092 |
| container_title | The Annals of occupational hygiene |
| container_volume | 60 |
| creator | Harari, Homero Bello, Dhimiter Woskie, Susan Redlich, Carrie |
| description | Objectives:
Skin is an important exposure route for isocyanate chemicals and contributes to systemic sensitization. Methods for assessing skin exposure are currently limited and generally rely upon removal (e.g. tape-strip) techniques prone to underestimation. The aim of this study is to (i) develop and field test an interception-based hand exposure sampler to monitor potential skin exposure to isocyanates in the workplace, (ii) to develop an analytical method based on ultra-high-performance liquid chromatography–UV absorbance–tandem mass spectrometry (UHPLC–UV–MS/MS) for analyzing glove samples; and (iii) compare it with tape-stripping skin sampling method.
Methods:
Laboratory investigations assessed different glove materials/fabrics, methods for impregnating with 1-(9-anthracenylmethyl)piperazine (MAP) derivatizing agent, methylene diphenyl diisocyanate (MDI) uptake and recovery, and durability. Following use, gloves were dissected into sections corresponding to different spatial regions (finger, palm) and analyzed using a newly developed UHPLC-UV-MS/MS method capable of differentiating and quantitating different MDI isomers with high sensitivity. Performance of the glove sampler was further assessed in a pilot field study using six workers.
Results:
A MAP-impregnated thin cotton glove sampler and UHPLC–UV–MS/MS analytical method for detecting MDI were successfully developed in laboratory studies. In subsequent field studies, a total of 384 samples from 14 glove pairs identified full-shift exposures ranged from 0.01 to 306 µg of 4,4′-MDI/worker for each hand. Surface area adjusted MDI values measured with the glove sampler (0.13–572ng MDI cm−2) were considerably higher (~400-fold) than values obtained with tape stripping.
Conclusion:
A glove sampler and a novel UHPLC-UV-MS/MS analytical method were developed to quantitatively measure MDI skin exposure. The novel interception technique overcomes inherent limitations of removal techniques for measuring isocyanate skin exposure and may be useful in exposure surveillance and future research on isocyanate’s health risks. |
| doi_str_mv | 10.1093/annhyg/mew052 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1835351076</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/annhyg/mew052</oup_id><sourcerecordid>1835351076</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-89e79ca42f83c4021f4636473e52357ae0294f15ecf6eef104bb1207bedc26d73</originalsourceid><addsrcrecordid>eNqFkDtPwzAURi0EoqUwsiKPLKF-xU7GCkqpVImhIMbIda8hkNjBTgr99xSlwMh0daWj80kHoXNKrijJ-Vg797J9HtfwQVJ2gIZUqDThVLJDNCSE8ERkKhugkxhfd6_gOT1GA6ZkLrigQ_R0AxuofFODa7G3WDs8dy0EA01beodnld8AXuq6qSBg6wNevpUOTz8bH7sAEbceT4KvdVsaPI_ebLXTLcRTdGR1FeFsf0fo8Xb6cH2XLO5n8-vJIjGcyTbJclC50YLZjBtBGLVCcikUh5TxVGkgLBeWpmCsBLCUiNWKMqJWsDZMrhUfocve2wT_3kFsi7qMBqpKO_BdLGjGU55SouQOTXrUBB9jAFs0oax12BaUFN8ti75l0bfc8Rd7dbeqYf1L_8T72_Zd84_rC8xyf_E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1835351076</pqid></control><display><type>article</type><title>Development of an Interception Glove Sampler for Skin Exposures to Aromatic Isocyanates</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Harari, Homero ; Bello, Dhimiter ; Woskie, Susan ; Redlich, Carrie</creator><creatorcontrib>Harari, Homero ; Bello, Dhimiter ; Woskie, Susan ; Redlich, Carrie</creatorcontrib><description>Objectives:
Skin is an important exposure route for isocyanate chemicals and contributes to systemic sensitization. Methods for assessing skin exposure are currently limited and generally rely upon removal (e.g. tape-strip) techniques prone to underestimation. The aim of this study is to (i) develop and field test an interception-based hand exposure sampler to monitor potential skin exposure to isocyanates in the workplace, (ii) to develop an analytical method based on ultra-high-performance liquid chromatography–UV absorbance–tandem mass spectrometry (UHPLC–UV–MS/MS) for analyzing glove samples; and (iii) compare it with tape-stripping skin sampling method.
Methods:
Laboratory investigations assessed different glove materials/fabrics, methods for impregnating with 1-(9-anthracenylmethyl)piperazine (MAP) derivatizing agent, methylene diphenyl diisocyanate (MDI) uptake and recovery, and durability. Following use, gloves were dissected into sections corresponding to different spatial regions (finger, palm) and analyzed using a newly developed UHPLC-UV-MS/MS method capable of differentiating and quantitating different MDI isomers with high sensitivity. Performance of the glove sampler was further assessed in a pilot field study using six workers.
Results:
A MAP-impregnated thin cotton glove sampler and UHPLC–UV–MS/MS analytical method for detecting MDI were successfully developed in laboratory studies. In subsequent field studies, a total of 384 samples from 14 glove pairs identified full-shift exposures ranged from 0.01 to 306 µg of 4,4′-MDI/worker for each hand. Surface area adjusted MDI values measured with the glove sampler (0.13–572ng MDI cm−2) were considerably higher (~400-fold) than values obtained with tape stripping.
Conclusion:
A glove sampler and a novel UHPLC-UV-MS/MS analytical method were developed to quantitatively measure MDI skin exposure. The novel interception technique overcomes inherent limitations of removal techniques for measuring isocyanate skin exposure and may be useful in exposure surveillance and future research on isocyanate’s health risks.</description><identifier>ISSN: 0003-4878</identifier><identifier>EISSN: 1475-3162</identifier><identifier>DOI: 10.1093/annhyg/mew052</identifier><identifier>PMID: 27694341</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Dermatitis, Contact - prevention & control ; Gloves, Protective - standards ; Humans ; Isocyanates - adverse effects ; Isocyanates - analysis ; Materials Testing - methods ; Occupational Exposure - prevention & control ; Skin Absorption - drug effects ; Tandem Mass Spectrometry - instrumentation ; Tandem Mass Spectrometry - methods</subject><ispartof>The Annals of occupational hygiene, 2016-11, Vol.60 (9), p.1092-1103</ispartof><rights>The Author 2016. Published by Oxford University Press on behalf of the British Occupational Hygiene Society. 2016</rights><rights>The Author 2016. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c326t-89e79ca42f83c4021f4636473e52357ae0294f15ecf6eef104bb1207bedc26d73</citedby><cites>FETCH-LOGICAL-c326t-89e79ca42f83c4021f4636473e52357ae0294f15ecf6eef104bb1207bedc26d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27694341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harari, Homero</creatorcontrib><creatorcontrib>Bello, Dhimiter</creatorcontrib><creatorcontrib>Woskie, Susan</creatorcontrib><creatorcontrib>Redlich, Carrie</creatorcontrib><title>Development of an Interception Glove Sampler for Skin Exposures to Aromatic Isocyanates</title><title>The Annals of occupational hygiene</title><addtitle>Ann Occup Hyg</addtitle><description>Objectives:
Skin is an important exposure route for isocyanate chemicals and contributes to systemic sensitization. Methods for assessing skin exposure are currently limited and generally rely upon removal (e.g. tape-strip) techniques prone to underestimation. The aim of this study is to (i) develop and field test an interception-based hand exposure sampler to monitor potential skin exposure to isocyanates in the workplace, (ii) to develop an analytical method based on ultra-high-performance liquid chromatography–UV absorbance–tandem mass spectrometry (UHPLC–UV–MS/MS) for analyzing glove samples; and (iii) compare it with tape-stripping skin sampling method.
Methods:
Laboratory investigations assessed different glove materials/fabrics, methods for impregnating with 1-(9-anthracenylmethyl)piperazine (MAP) derivatizing agent, methylene diphenyl diisocyanate (MDI) uptake and recovery, and durability. Following use, gloves were dissected into sections corresponding to different spatial regions (finger, palm) and analyzed using a newly developed UHPLC-UV-MS/MS method capable of differentiating and quantitating different MDI isomers with high sensitivity. Performance of the glove sampler was further assessed in a pilot field study using six workers.
Results:
A MAP-impregnated thin cotton glove sampler and UHPLC–UV–MS/MS analytical method for detecting MDI were successfully developed in laboratory studies. In subsequent field studies, a total of 384 samples from 14 glove pairs identified full-shift exposures ranged from 0.01 to 306 µg of 4,4′-MDI/worker for each hand. Surface area adjusted MDI values measured with the glove sampler (0.13–572ng MDI cm−2) were considerably higher (~400-fold) than values obtained with tape stripping.
Conclusion:
A glove sampler and a novel UHPLC-UV-MS/MS analytical method were developed to quantitatively measure MDI skin exposure. The novel interception technique overcomes inherent limitations of removal techniques for measuring isocyanate skin exposure and may be useful in exposure surveillance and future research on isocyanate’s health risks.</description><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dermatitis, Contact - prevention & control</subject><subject>Gloves, Protective - standards</subject><subject>Humans</subject><subject>Isocyanates - adverse effects</subject><subject>Isocyanates - analysis</subject><subject>Materials Testing - methods</subject><subject>Occupational Exposure - prevention & control</subject><subject>Skin Absorption - drug effects</subject><subject>Tandem Mass Spectrometry - instrumentation</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0003-4878</issn><issn>1475-3162</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAURi0EoqUwsiKPLKF-xU7GCkqpVImhIMbIda8hkNjBTgr99xSlwMh0daWj80kHoXNKrijJ-Vg797J9HtfwQVJ2gIZUqDThVLJDNCSE8ERkKhugkxhfd6_gOT1GA6ZkLrigQ_R0AxuofFODa7G3WDs8dy0EA01beodnld8AXuq6qSBg6wNevpUOTz8bH7sAEbceT4KvdVsaPI_ebLXTLcRTdGR1FeFsf0fo8Xb6cH2XLO5n8-vJIjGcyTbJclC50YLZjBtBGLVCcikUh5TxVGkgLBeWpmCsBLCUiNWKMqJWsDZMrhUfocve2wT_3kFsi7qMBqpKO_BdLGjGU55SouQOTXrUBB9jAFs0oax12BaUFN8ti75l0bfc8Rd7dbeqYf1L_8T72_Zd84_rC8xyf_E</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Harari, Homero</creator><creator>Bello, Dhimiter</creator><creator>Woskie, Susan</creator><creator>Redlich, Carrie</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20161101</creationdate><title>Development of an Interception Glove Sampler for Skin Exposures to Aromatic Isocyanates</title><author>Harari, Homero ; Bello, Dhimiter ; Woskie, Susan ; Redlich, Carrie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-89e79ca42f83c4021f4636473e52357ae0294f15ecf6eef104bb1207bedc26d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Dermatitis, Contact - prevention & control</topic><topic>Gloves, Protective - standards</topic><topic>Humans</topic><topic>Isocyanates - adverse effects</topic><topic>Isocyanates - analysis</topic><topic>Materials Testing - methods</topic><topic>Occupational Exposure - prevention & control</topic><topic>Skin Absorption - drug effects</topic><topic>Tandem Mass Spectrometry - instrumentation</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>online_resources</toplevel><creatorcontrib>Harari, Homero</creatorcontrib><creatorcontrib>Bello, Dhimiter</creatorcontrib><creatorcontrib>Woskie, Susan</creatorcontrib><creatorcontrib>Redlich, Carrie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Annals of occupational hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harari, Homero</au><au>Bello, Dhimiter</au><au>Woskie, Susan</au><au>Redlich, Carrie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an Interception Glove Sampler for Skin Exposures to Aromatic Isocyanates</atitle><jtitle>The Annals of occupational hygiene</jtitle><addtitle>Ann Occup Hyg</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>60</volume><issue>9</issue><spage>1092</spage><epage>1103</epage><pages>1092-1103</pages><issn>0003-4878</issn><eissn>1475-3162</eissn><abstract>Objectives:
Skin is an important exposure route for isocyanate chemicals and contributes to systemic sensitization. Methods for assessing skin exposure are currently limited and generally rely upon removal (e.g. tape-strip) techniques prone to underestimation. The aim of this study is to (i) develop and field test an interception-based hand exposure sampler to monitor potential skin exposure to isocyanates in the workplace, (ii) to develop an analytical method based on ultra-high-performance liquid chromatography–UV absorbance–tandem mass spectrometry (UHPLC–UV–MS/MS) for analyzing glove samples; and (iii) compare it with tape-stripping skin sampling method.
Methods:
Laboratory investigations assessed different glove materials/fabrics, methods for impregnating with 1-(9-anthracenylmethyl)piperazine (MAP) derivatizing agent, methylene diphenyl diisocyanate (MDI) uptake and recovery, and durability. Following use, gloves were dissected into sections corresponding to different spatial regions (finger, palm) and analyzed using a newly developed UHPLC-UV-MS/MS method capable of differentiating and quantitating different MDI isomers with high sensitivity. Performance of the glove sampler was further assessed in a pilot field study using six workers.
Results:
A MAP-impregnated thin cotton glove sampler and UHPLC–UV–MS/MS analytical method for detecting MDI were successfully developed in laboratory studies. In subsequent field studies, a total of 384 samples from 14 glove pairs identified full-shift exposures ranged from 0.01 to 306 µg of 4,4′-MDI/worker for each hand. Surface area adjusted MDI values measured with the glove sampler (0.13–572ng MDI cm−2) were considerably higher (~400-fold) than values obtained with tape stripping.
Conclusion:
A glove sampler and a novel UHPLC-UV-MS/MS analytical method were developed to quantitatively measure MDI skin exposure. The novel interception technique overcomes inherent limitations of removal techniques for measuring isocyanate skin exposure and may be useful in exposure surveillance and future research on isocyanate’s health risks.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27694341</pmid><doi>10.1093/annhyg/mew052</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-4878 |
| ispartof | The Annals of occupational hygiene, 2016-11, Vol.60 (9), p.1092-1103 |
| issn | 0003-4878 1475-3162 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835351076 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Dermatitis, Contact - prevention & control Gloves, Protective - standards Humans Isocyanates - adverse effects Isocyanates - analysis Materials Testing - methods Occupational Exposure - prevention & control Skin Absorption - drug effects Tandem Mass Spectrometry - instrumentation Tandem Mass Spectrometry - methods |
| title | Development of an Interception Glove Sampler for Skin Exposures to Aromatic Isocyanates |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T18%3A29%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20an%20Interception%20Glove%20Sampler%20for%20Skin%20Exposures%20to%20Aromatic%20Isocyanates&rft.jtitle=The%20Annals%20of%20occupational%20hygiene&rft.au=Harari,%20Homero&rft.date=2016-11-01&rft.volume=60&rft.issue=9&rft.spage=1092&rft.epage=1103&rft.pages=1092-1103&rft.issn=0003-4878&rft.eissn=1475-3162&rft_id=info:doi/10.1093/annhyg/mew052&rft_dat=%3Cproquest_cross%3E1835351076%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1835351076&rft_id=info:pmid/27694341&rft_oup_id=10.1093/annhyg/mew052&rfr_iscdi=true |