WDR26 promotes mitophagy of cardiomyocytes induced by hypoxia through Parkin translocation
Myocardial ischemia is a heart condition caused by reduction of blood flow to the heart, preventing heart from receiving enough oxygen. Myocardial ischemia is the most common cause of death globally. Heart ischemic preconditioning （IPC） has a protective effect against myocardial cell death induced b...
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| Veröffentlicht in: | Acta biochimica et biophysica Sinica 2016-12, Vol.48 (12), p.1075-1084 |
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| container_title | Acta biochimica et biophysica Sinica |
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| creator | Feng, Yansheng Zhao, Jia Hou, Huifang Zhang, Hui Jiao, Yunjuan Wang, Jiangang Wang, Yongling Sun, Yinping |
| description | Myocardial ischemia is a heart condition caused by reduction of blood flow to the heart, preventing heart from receiving enough oxygen. Myocardial ischemia is the most common cause of death globally. Heart ischemic preconditioning （IPC） has a protective effect against myocardial cell death induced by ischemia and ischemia-reperfusion injury. WDR26 has recently been identified as a protein that is increased following rat cardiac IPC. WDR26 can promote the proliferation of H9c2 cells and protect cardiomyocytes against oxidative stress through inhibiting apoptosis. However, its role in myocardial ischemia is unclear. The aim of this study was to explore the role of WDR26 in myocardial ischemia and H9c2 cell hypoxia. Our results showed that WDR26 is induced by myocardial ischemia and H9c2 cell hypoxia. WDR26 protects H9c2 cells against hypoxia injury through inhibiting LDH release and increasing cell viability. WDR26 promotes hypoxia-induced autophagy in hypoxia of H9c2 cells. We further demonstrated that in H9c2 cell hypoxia, WDR26 increases mitochondrial membrane potential, thereby increases Parkin translocation of mitochondria. After Parkin is translocated at mitochondria, WDR26 can increase mitochondrial protein ubiquitination in hypoxia of H9c2 cells. WDR26 is a mediator of response to hypoxia, and WDR26 plays an important role in hypoxia-mediated autophagy and mitophagy. This study provides novel insights into the protective role of WDR26 in cardiomyocyte injury during hypoxia. WDR26 may serve as a potential target for the treatment of myocardial ischemia. |
| doi_str_mv | 10.1093/abbs/gmw104 |
| format | Article |
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Myocardial ischemia is the most common cause of death globally. Heart ischemic preconditioning （IPC） has a protective effect against myocardial cell death induced by ischemia and ischemia-reperfusion injury. WDR26 has recently been identified as a protein that is increased following rat cardiac IPC. WDR26 can promote the proliferation of H9c2 cells and protect cardiomyocytes against oxidative stress through inhibiting apoptosis. However, its role in myocardial ischemia is unclear. The aim of this study was to explore the role of WDR26 in myocardial ischemia and H9c2 cell hypoxia. Our results showed that WDR26 is induced by myocardial ischemia and H9c2 cell hypoxia. WDR26 protects H9c2 cells against hypoxia injury through inhibiting LDH release and increasing cell viability. WDR26 promotes hypoxia-induced autophagy in hypoxia of H9c2 cells. We further demonstrated that in H9c2 cell hypoxia, WDR26 increases mitochondrial membrane potential, thereby increases Parkin translocation of mitochondria. After Parkin is translocated at mitochondria, WDR26 can increase mitochondrial protein ubiquitination in hypoxia of H9c2 cells. WDR26 is a mediator of response to hypoxia, and WDR26 plays an important role in hypoxia-mediated autophagy and mitophagy. This study provides novel insights into the protective role of WDR26 in cardiomyocyte injury during hypoxia. WDR26 may serve as a potential target for the treatment of myocardial ischemia.</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1093/abbs/gmw104</identifier><identifier>PMID: 27797717</identifier><language>eng</language><publisher>China</publisher><subject>Animals ; Cell Hypoxia ; Cell Line ; Membrane Potential, Mitochondrial ; Mitochondrial Degradation - physiology ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - metabolism ; Protein Transport ; Proteins - physiology ; Rats ; Rats, Sprague-Dawley ; Ubiquitin-Protein Ligases - metabolism ; 心肌细胞损伤 ; 心肌缺血 ; 易位 ; 线粒体膜电位 ; 细胞缺氧 ; 缺血再灌注损伤 ; 自噬 ; 诱导</subject><ispartof>Acta biochimica et biophysica Sinica, 2016-12, Vol.48 (12), p.1075-1084</ispartof><rights>The Author 2016. Published by Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-997d2b4ef8c47feff1b3b98b1673f78b4b8128030521d8117d286ce19470a28c3</citedby><cites>FETCH-LOGICAL-c353t-997d2b4ef8c47feff1b3b98b1673f78b4b8128030521d8117d286ce19470a28c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90160X/90160X.jpg</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27797717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Yansheng</creatorcontrib><creatorcontrib>Zhao, Jia</creatorcontrib><creatorcontrib>Hou, Huifang</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Jiao, Yunjuan</creatorcontrib><creatorcontrib>Wang, Jiangang</creatorcontrib><creatorcontrib>Wang, Yongling</creatorcontrib><creatorcontrib>Sun, Yinping</creatorcontrib><title>WDR26 promotes mitophagy of cardiomyocytes induced by hypoxia through Parkin translocation</title><title>Acta biochimica et biophysica Sinica</title><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><description>Myocardial ischemia is a heart condition caused by reduction of blood flow to the heart, preventing heart from receiving enough oxygen. Myocardial ischemia is the most common cause of death globally. Heart ischemic preconditioning （IPC） has a protective effect against myocardial cell death induced by ischemia and ischemia-reperfusion injury. WDR26 has recently been identified as a protein that is increased following rat cardiac IPC. WDR26 can promote the proliferation of H9c2 cells and protect cardiomyocytes against oxidative stress through inhibiting apoptosis. However, its role in myocardial ischemia is unclear. The aim of this study was to explore the role of WDR26 in myocardial ischemia and H9c2 cell hypoxia. Our results showed that WDR26 is induced by myocardial ischemia and H9c2 cell hypoxia. WDR26 protects H9c2 cells against hypoxia injury through inhibiting LDH release and increasing cell viability. WDR26 promotes hypoxia-induced autophagy in hypoxia of H9c2 cells. We further demonstrated that in H9c2 cell hypoxia, WDR26 increases mitochondrial membrane potential, thereby increases Parkin translocation of mitochondria. After Parkin is translocated at mitochondria, WDR26 can increase mitochondrial protein ubiquitination in hypoxia of H9c2 cells. WDR26 is a mediator of response to hypoxia, and WDR26 plays an important role in hypoxia-mediated autophagy and mitophagy. This study provides novel insights into the protective role of WDR26 in cardiomyocyte injury during hypoxia. WDR26 may serve as a potential target for the treatment of myocardial ischemia.</description><subject>Animals</subject><subject>Cell Hypoxia</subject><subject>Cell Line</subject><subject>Membrane Potential, Mitochondrial</subject><subject>Mitochondrial Degradation - physiology</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Protein Transport</subject><subject>Proteins - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Ubiquitin-Protein Ligases - metabolism</subject><subject>心肌细胞损伤</subject><subject>心肌缺血</subject><subject>易位</subject><subject>线粒体膜电位</subject><subject>细胞缺氧</subject><subject>缺血再灌注损伤</subject><subject>自噬</subject><subject>诱导</subject><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMoun6cvEvwJEg106RNepT1EwRFFMFLSdK0jbbNmrRo_71ddt3TDMzDOzMPQsdALoBk9FIqFS6r9gcI20Iz4CyJeMzJ9tSnPI4yYMke2g_hkxCapkB20V7MecY58Bn6eL9-iVO88K51vQm4tb1b1LIasSuxlr6wrh2dHpcz2xWDNgVWI67Hhfu1Eve1d0NV42fpv2yHey-70Dgte-u6Q7RTyiaYo3U9QG-3N6_z--jx6e5hfvUYaZrQPsoyXsSKmVJoxktTlqCoyoSajqclF4opAbEglCQxFAJgokWqDWSMExkLTQ_Q2Sp3euJ7MKHPWxu0aRrZGTeEHARl05KUiAk9X6HauxC8KfOFt630Yw4kX8rMlzLzlcyJPlkHD6o1xYb9tzcBp-u42nXVt-2qDZNygASYSOgfoAV9OA</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Feng, Yansheng</creator><creator>Zhao, Jia</creator><creator>Hou, Huifang</creator><creator>Zhang, Hui</creator><creator>Jiao, Yunjuan</creator><creator>Wang, Jiangang</creator><creator>Wang, Yongling</creator><creator>Sun, Yinping</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20161201</creationdate><title>WDR26 promotes mitophagy of cardiomyocytes induced by hypoxia through Parkin translocation</title><author>Feng, Yansheng ; Zhao, Jia ; Hou, Huifang ; Zhang, Hui ; Jiao, Yunjuan ; Wang, Jiangang ; Wang, Yongling ; Sun, Yinping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-997d2b4ef8c47feff1b3b98b1673f78b4b8128030521d8117d286ce19470a28c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Cell Hypoxia</topic><topic>Cell Line</topic><topic>Membrane Potential, Mitochondrial</topic><topic>Mitochondrial Degradation - physiology</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Protein Transport</topic><topic>Proteins - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Ubiquitin-Protein Ligases - metabolism</topic><topic>心肌细胞损伤</topic><topic>心肌缺血</topic><topic>易位</topic><topic>线粒体膜电位</topic><topic>细胞缺氧</topic><topic>缺血再灌注损伤</topic><topic>自噬</topic><topic>诱导</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Yansheng</creatorcontrib><creatorcontrib>Zhao, Jia</creatorcontrib><creatorcontrib>Hou, Huifang</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Jiao, Yunjuan</creatorcontrib><creatorcontrib>Wang, Jiangang</creatorcontrib><creatorcontrib>Wang, Yongling</creatorcontrib><creatorcontrib>Sun, Yinping</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Yansheng</au><au>Zhao, Jia</au><au>Hou, Huifang</au><au>Zhang, Hui</au><au>Jiao, Yunjuan</au><au>Wang, Jiangang</au><au>Wang, Yongling</au><au>Sun, Yinping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>WDR26 promotes mitophagy of cardiomyocytes induced by hypoxia through Parkin translocation</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>48</volume><issue>12</issue><spage>1075</spage><epage>1084</epage><pages>1075-1084</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>Myocardial ischemia is a heart condition caused by reduction of blood flow to the heart, preventing heart from receiving enough oxygen. Myocardial ischemia is the most common cause of death globally. Heart ischemic preconditioning （IPC） has a protective effect against myocardial cell death induced by ischemia and ischemia-reperfusion injury. WDR26 has recently been identified as a protein that is increased following rat cardiac IPC. WDR26 can promote the proliferation of H9c2 cells and protect cardiomyocytes against oxidative stress through inhibiting apoptosis. However, its role in myocardial ischemia is unclear. The aim of this study was to explore the role of WDR26 in myocardial ischemia and H9c2 cell hypoxia. Our results showed that WDR26 is induced by myocardial ischemia and H9c2 cell hypoxia. WDR26 protects H9c2 cells against hypoxia injury through inhibiting LDH release and increasing cell viability. WDR26 promotes hypoxia-induced autophagy in hypoxia of H9c2 cells. We further demonstrated that in H9c2 cell hypoxia, WDR26 increases mitochondrial membrane potential, thereby increases Parkin translocation of mitochondria. After Parkin is translocated at mitochondria, WDR26 can increase mitochondrial protein ubiquitination in hypoxia of H9c2 cells. WDR26 is a mediator of response to hypoxia, and WDR26 plays an important role in hypoxia-mediated autophagy and mitophagy. This study provides novel insights into the protective role of WDR26 in cardiomyocyte injury during hypoxia. WDR26 may serve as a potential target for the treatment of myocardial ischemia.</abstract><cop>China</cop><pmid>27797717</pmid><doi>10.1093/abbs/gmw104</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cell Hypoxia Cell Line Membrane Potential, Mitochondrial Mitochondrial Degradation - physiology Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Protein Transport Proteins - physiology Rats Rats, Sprague-Dawley Ubiquitin-Protein Ligases - metabolism 心肌细胞损伤 心肌缺血 易位 线粒体膜电位 细胞缺氧 缺血再灌注损伤 自噬 诱导 |
| title | WDR26 promotes mitophagy of cardiomyocytes induced by hypoxia through Parkin translocation |
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