First Molecular Identification of Dirofilaria repens in a Dog Blood Sample from Guanajuato, Mexico
Dirofilaria repens is the causative zoonotic agent of subcutaneous dirofilariosis. Although the American continent is considered currently free of D. repens infections, in the past few years there have been some reports suggesting the presence of this agent in the Americas. In Guanajuato, Mexico, th...
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| Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2016-11, Vol.16 (11), p.734-736 |
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| container_title | Vector borne and zoonotic diseases (Larchmont, N.Y.) |
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| creator | Ramos-Lopez, Sandra León-Galván, Ma. Fabiola Salas-Alatorre, Mariana Lechuga-Arana, Alma A. Valencia-Posadas, Mauricio Gutiérrez-Chávez, Abner J. |
| description | Dirofilaria repens
is the causative zoonotic agent of subcutaneous dirofilariosis. Although the American continent is considered currently free of
D. repens
infections, in the past few years there have been some reports suggesting the presence of this agent in the Americas. In Guanajuato, Mexico, there is a report of the presence of
D. repens
. To determine whether this parasite is really present in Guanajuato, a total of 177 EDTA blood samples from dogs were tested using the modified Knott's technique. Using this technique, we found 15 positive samples, which were then analyzed using the PCR technique, with a panfilarial set of primers and a specific primer pair for
D. repens
. In one sample, the expected band was present and the corresponding amplicon, sequenced, displayed a 100% identity to
D. repens
, confirming the presence of this exotic and zoonotic filarial species in the municipality of Silao, Guanajuato, Mexico. |
| doi_str_mv | 10.1089/vbz.2016.1948 |
| format | Article |
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is the causative zoonotic agent of subcutaneous dirofilariosis. Although the American continent is considered currently free of
D. repens
infections, in the past few years there have been some reports suggesting the presence of this agent in the Americas. In Guanajuato, Mexico, there is a report of the presence of
D. repens
. To determine whether this parasite is really present in Guanajuato, a total of 177 EDTA blood samples from dogs were tested using the modified Knott's technique. Using this technique, we found 15 positive samples, which were then analyzed using the PCR technique, with a panfilarial set of primers and a specific primer pair for
D. repens
. In one sample, the expected band was present and the corresponding amplicon, sequenced, displayed a 100% identity to
D. repens
, confirming the presence of this exotic and zoonotic filarial species in the municipality of Silao, Guanajuato, Mexico.</description><identifier>ISSN: 1530-3667</identifier><identifier>EISSN: 1557-7759</identifier><identifier>DOI: 10.1089/vbz.2016.1948</identifier><identifier>PMID: 27631566</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Dirofilaria repens - isolation & purification ; Dirofilariasis - blood ; Dirofilariasis - epidemiology ; Dirofilariasis - parasitology ; Dog Diseases - blood ; Dog Diseases - epidemiology ; Dog Diseases - parasitology ; Dogs ; Polymerase Chain Reaction ; Short Communications ; Zoonoses</subject><ispartof>Vector borne and zoonotic diseases (Larchmont, N.Y.), 2016-11, Vol.16 (11), p.734-736</ispartof><rights>2016, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-55f38b8ea0b3f28500cd674d99cbdd6991a750d6e4159ba88519c4d511cbe9573</citedby><cites>FETCH-LOGICAL-c337t-55f38b8ea0b3f28500cd674d99cbdd6991a750d6e4159ba88519c4d511cbe9573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27631566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramos-Lopez, Sandra</creatorcontrib><creatorcontrib>León-Galván, Ma. Fabiola</creatorcontrib><creatorcontrib>Salas-Alatorre, Mariana</creatorcontrib><creatorcontrib>Lechuga-Arana, Alma A.</creatorcontrib><creatorcontrib>Valencia-Posadas, Mauricio</creatorcontrib><creatorcontrib>Gutiérrez-Chávez, Abner J.</creatorcontrib><title>First Molecular Identification of Dirofilaria repens in a Dog Blood Sample from Guanajuato, Mexico</title><title>Vector borne and zoonotic diseases (Larchmont, N.Y.)</title><addtitle>Vector Borne Zoonotic Dis</addtitle><description>Dirofilaria repens
is the causative zoonotic agent of subcutaneous dirofilariosis. Although the American continent is considered currently free of
D. repens
infections, in the past few years there have been some reports suggesting the presence of this agent in the Americas. In Guanajuato, Mexico, there is a report of the presence of
D. repens
. To determine whether this parasite is really present in Guanajuato, a total of 177 EDTA blood samples from dogs were tested using the modified Knott's technique. Using this technique, we found 15 positive samples, which were then analyzed using the PCR technique, with a panfilarial set of primers and a specific primer pair for
D. repens
. In one sample, the expected band was present and the corresponding amplicon, sequenced, displayed a 100% identity to
D. repens
, confirming the presence of this exotic and zoonotic filarial species in the municipality of Silao, Guanajuato, Mexico.</description><subject>Animals</subject><subject>Dirofilaria repens - isolation & purification</subject><subject>Dirofilariasis - blood</subject><subject>Dirofilariasis - epidemiology</subject><subject>Dirofilariasis - parasitology</subject><subject>Dog Diseases - blood</subject><subject>Dog Diseases - epidemiology</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>Polymerase Chain Reaction</subject><subject>Short Communications</subject><subject>Zoonoses</subject><issn>1530-3667</issn><issn>1557-7759</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLlOxDAQQC0E4lgoaZFLCrLY6_gquUFaRAHUkY8JMkrixU4Q8PUkWqClmtHM0yseQoeUzClR-vTdfs0XhIo51aXaQLuUc1lIyfXmtDNSMCHkDtrL-ZWQBVWUb6OdhRSMciF2kb0OKff4PjbghsYkfOeh60MdnOlD7HCs8WVIsQ7jLxicYAVdxqHDBl_GF3zexOjxo2lXDeA6xRbfDKYzr4Pp4wm-h4_g4j7aqk2T4eBnztDz9dXTxW2xfLi5uzhbFo4x2Rec10xZBYZYVi8UJ8R5IUuvtbPeC62pkZx4ASXl2hqlONWu9JxSZ0FzyWboeO1dpfg2QO6rNmQHTWM6iEOuqGKl1lwoMqLFGnUp5pygrlYptCZ9VpRUU9ZqzFpNWasp68gf_agH24L_o387jgBbA9PZdF0TwELq_9F-A9QDhI8</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Ramos-Lopez, Sandra</creator><creator>León-Galván, Ma. Fabiola</creator><creator>Salas-Alatorre, Mariana</creator><creator>Lechuga-Arana, Alma A.</creator><creator>Valencia-Posadas, Mauricio</creator><creator>Gutiérrez-Chávez, Abner J.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20161101</creationdate><title>First Molecular Identification of Dirofilaria repens in a Dog Blood Sample from Guanajuato, Mexico</title><author>Ramos-Lopez, Sandra ; León-Galván, Ma. Fabiola ; Salas-Alatorre, Mariana ; Lechuga-Arana, Alma A. ; Valencia-Posadas, Mauricio ; Gutiérrez-Chávez, Abner J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-55f38b8ea0b3f28500cd674d99cbdd6991a750d6e4159ba88519c4d511cbe9573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Dirofilaria repens - isolation & purification</topic><topic>Dirofilariasis - blood</topic><topic>Dirofilariasis - epidemiology</topic><topic>Dirofilariasis - parasitology</topic><topic>Dog Diseases - blood</topic><topic>Dog Diseases - epidemiology</topic><topic>Dog Diseases - parasitology</topic><topic>Dogs</topic><topic>Polymerase Chain Reaction</topic><topic>Short Communications</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramos-Lopez, Sandra</creatorcontrib><creatorcontrib>León-Galván, Ma. Fabiola</creatorcontrib><creatorcontrib>Salas-Alatorre, Mariana</creatorcontrib><creatorcontrib>Lechuga-Arana, Alma A.</creatorcontrib><creatorcontrib>Valencia-Posadas, Mauricio</creatorcontrib><creatorcontrib>Gutiérrez-Chávez, Abner J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramos-Lopez, Sandra</au><au>León-Galván, Ma. Fabiola</au><au>Salas-Alatorre, Mariana</au><au>Lechuga-Arana, Alma A.</au><au>Valencia-Posadas, Mauricio</au><au>Gutiérrez-Chávez, Abner J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First Molecular Identification of Dirofilaria repens in a Dog Blood Sample from Guanajuato, Mexico</atitle><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle><addtitle>Vector Borne Zoonotic Dis</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>16</volume><issue>11</issue><spage>734</spage><epage>736</epage><pages>734-736</pages><issn>1530-3667</issn><eissn>1557-7759</eissn><abstract>Dirofilaria repens
is the causative zoonotic agent of subcutaneous dirofilariosis. Although the American continent is considered currently free of
D. repens
infections, in the past few years there have been some reports suggesting the presence of this agent in the Americas. In Guanajuato, Mexico, there is a report of the presence of
D. repens
. To determine whether this parasite is really present in Guanajuato, a total of 177 EDTA blood samples from dogs were tested using the modified Knott's technique. Using this technique, we found 15 positive samples, which were then analyzed using the PCR technique, with a panfilarial set of primers and a specific primer pair for
D. repens
. In one sample, the expected band was present and the corresponding amplicon, sequenced, displayed a 100% identity to
D. repens
, confirming the presence of this exotic and zoonotic filarial species in the municipality of Silao, Guanajuato, Mexico.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>27631566</pmid><doi>10.1089/vbz.2016.1948</doi><tpages>3</tpages></addata></record> |
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| ispartof | Vector borne and zoonotic diseases (Larchmont, N.Y.), 2016-11, Vol.16 (11), p.734-736 |
| issn | 1530-3667 1557-7759 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Dirofilaria repens - isolation & purification Dirofilariasis - blood Dirofilariasis - epidemiology Dirofilariasis - parasitology Dog Diseases - blood Dog Diseases - epidemiology Dog Diseases - parasitology Dogs Polymerase Chain Reaction Short Communications Zoonoses |
| title | First Molecular Identification of Dirofilaria repens in a Dog Blood Sample from Guanajuato, Mexico |
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