Phage display selection of hairpin loop soyacystatin variants that mediate high affinity inhibition of a cysteine proteinase

Summary Two hairpin‐loop domains in cystatin family proteinase inhibitors form an interface surface region that slots into the active site cleft of papain‐like cysteine proteinases, and determine binding affinity. The slot region surface architecture of the soybean cysteine proteinase inhibitor (soy...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 2001-09, Vol.27 (5), p.383-391
Hauptverfasser:	Koiwa, Hisashi, D'Urzo, Matilde Paino, Assfalg‐Machleidt, Irmgard, Zhu‐Salzman, Keyan, Shade, Richard E., An, Haejung, Murdock, Larry L., Machleidt, Werner, Bressan, Ray A., Hasegawa, Paul M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences cystatin Cystatins - genetics Cystatins - metabolism cysteine proteinase Cysteine Proteinase Inhibitors - genetics Cysteine Proteinase Inhibitors - metabolism Directed Molecular Evolution Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Genetic Variation Glycine max Hydrolases molecular evolution Molecular Sequence Data Mutagenesis Mutation Papain - antagonists & inhibitors Peptide Library phage display Protein Structure, Secondary proteinase inhibitor Recombinant Proteins - metabolism soyacystatin Soybean Proteins
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