PHOSPHATIDYLINOSITOL 3-KINASE REGULATES PROLIFERATION OF RAW 264.7 MACROPHAGES
Phosphatidylinositol 3-kinase (PI3-kinase) is an enzyme that acts as a direct biochemical link between a novel phosphatidylinositol pathway and a number of proteins containing intrinsic or associated kinase activities. Here we demonstrate that wortmannin, PI3-kinase inhibitor, decreases the prolifer...
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| Veröffentlicht in: | Immunopharmacology and immunotoxicology 2001-01, Vol.23 (3), p.355-365 |
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| creator | Kim, Hyung-Min Yim, Hee-Gyun Yoon, Hyang-Suk Park, Seung-Taeck Jeung, Jae-Yeal Lee, Ki-Nam Baek, Seung-Hwa Song, Yung-Sun Oh, Gyung-Jae Kim, Nam-Song An, Nyeon-Hyoung |
| description | Phosphatidylinositol 3-kinase (PI3-kinase) is an enzyme that acts as a direct biochemical link between a novel phosphatidylinositol pathway and a number of proteins containing intrinsic or associated kinase activities. Here we demonstrate that wortmannin, PI3-kinase inhibitor, decreases the proliferation of RAW 264.7 macrophages and that another structurally unrelated inhibitor of PI3-kinase, LY294002, also inhibits the proliferation. These results indicate a possible involvement of PI3-kinase in RAW 264.7 macrophages growth regulation. Wortmannin stimulation of RAW 264.7 macrophages is followed by sustained expression of the mRNA of c-fos and a transient expression of the mRNA of c-jun. We also show that the wortmannin and LY294002 induce a cell cycle arrest in asynchronously growing cells leading to an inhibition of cell proliferation after 12 h of treatment. In addition, wortmannin or LY294002 inhibited the phorbol 12-myristate 13-acetate-induced macrophages proliferation potently. These results suggest that PI3-kinase plays an important role in growth regulation of RAW 264.7 macrophages and that protein kinase C is a down stream effector of PI3-kinase. |
| doi_str_mv | 10.1081/IPH-100107336 |
| format | Article |
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Here we demonstrate that wortmannin, PI3-kinase inhibitor, decreases the proliferation of RAW 264.7 macrophages and that another structurally unrelated inhibitor of PI3-kinase, LY294002, also inhibits the proliferation. These results indicate a possible involvement of PI3-kinase in RAW 264.7 macrophages growth regulation. Wortmannin stimulation of RAW 264.7 macrophages is followed by sustained expression of the mRNA of c-fos and a transient expression of the mRNA of c-jun. We also show that the wortmannin and LY294002 induce a cell cycle arrest in asynchronously growing cells leading to an inhibition of cell proliferation after 12 h of treatment. In addition, wortmannin or LY294002 inhibited the phorbol 12-myristate 13-acetate-induced macrophages proliferation potently. These results suggest that PI3-kinase plays an important role in growth regulation of RAW 264.7 macrophages and that protein kinase C is a down stream effector of PI3-kinase.</description><identifier>ISSN: 0892-3973</identifier><identifier>EISSN: 1532-2513</identifier><identifier>DOI: 10.1081/IPH-100107336</identifier><identifier>PMID: 11694027</identifier><identifier>CODEN: IITOEF</identifier><language>eng</language><publisher>Monticello, NY: Informa UK Ltd</publisher><subject>Androstadienes - pharmacology ; Animals ; Biological and medical sciences ; c-fos ; c-jun ; Cell Cycle - drug effects ; Cell cycle, cell proliferation ; Cell Division - drug effects ; Cell Division - physiology ; Cell Line ; Cell physiology ; Chromones - pharmacology ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene Expression - drug effects ; Genes, fos - drug effects ; Genes, jun - drug effects ; Macrophages - cytology ; Macrophages - drug effects ; Macrophages - enzymology ; Mice ; Molecular and cellular biology ; Morpholines - pharmacology ; phorbol 12-myristate 13-acetate ; Phosphatidylinositol 3-kinase ; Phosphatidylinositol 3-Kinases - antagonists & inhibitors ; Phosphatidylinositol 3-Kinases - physiology ; Protein kinase C ; RAW 264.7 macrophages ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Tetradecanoylphorbol Acetate - pharmacology ; wortmannin</subject><ispartof>Immunopharmacology and immunotoxicology, 2001-01, Vol.23 (3), p.355-365</ispartof><rights>2001 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2001</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c372t-c7ac290adab7f5f9ac5df7c94046342cb26ec12a55e15c9b5ae585d5c5246a423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1081/IPH-100107336$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1081/IPH-100107336$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13887273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11694027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hyung-Min</creatorcontrib><creatorcontrib>Yim, Hee-Gyun</creatorcontrib><creatorcontrib>Yoon, Hyang-Suk</creatorcontrib><creatorcontrib>Park, Seung-Taeck</creatorcontrib><creatorcontrib>Jeung, Jae-Yeal</creatorcontrib><creatorcontrib>Lee, Ki-Nam</creatorcontrib><creatorcontrib>Baek, Seung-Hwa</creatorcontrib><creatorcontrib>Song, Yung-Sun</creatorcontrib><creatorcontrib>Oh, Gyung-Jae</creatorcontrib><creatorcontrib>Kim, Nam-Song</creatorcontrib><creatorcontrib>An, Nyeon-Hyoung</creatorcontrib><title>PHOSPHATIDYLINOSITOL 3-KINASE REGULATES PROLIFERATION OF RAW 264.7 MACROPHAGES</title><title>Immunopharmacology and immunotoxicology</title><addtitle>Immunopharmacol Immunotoxicol</addtitle><description>Phosphatidylinositol 3-kinase (PI3-kinase) is an enzyme that acts as a direct biochemical link between a novel phosphatidylinositol pathway and a number of proteins containing intrinsic or associated kinase activities. Here we demonstrate that wortmannin, PI3-kinase inhibitor, decreases the proliferation of RAW 264.7 macrophages and that another structurally unrelated inhibitor of PI3-kinase, LY294002, also inhibits the proliferation. These results indicate a possible involvement of PI3-kinase in RAW 264.7 macrophages growth regulation. Wortmannin stimulation of RAW 264.7 macrophages is followed by sustained expression of the mRNA of c-fos and a transient expression of the mRNA of c-jun. We also show that the wortmannin and LY294002 induce a cell cycle arrest in asynchronously growing cells leading to an inhibition of cell proliferation after 12 h of treatment. In addition, wortmannin or LY294002 inhibited the phorbol 12-myristate 13-acetate-induced macrophages proliferation potently. These results suggest that PI3-kinase plays an important role in growth regulation of RAW 264.7 macrophages and that protein kinase C is a down stream effector of PI3-kinase.</description><subject>Androstadienes - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>c-fos</subject><subject>c-jun</subject><subject>Cell Cycle - drug effects</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - physiology</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Chromones - pharmacology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - drug effects</subject><subject>Genes, fos - drug effects</subject><subject>Genes, jun - drug effects</subject><subject>Macrophages - cytology</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - enzymology</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Morpholines - pharmacology</subject><subject>phorbol 12-myristate 13-acetate</subject><subject>Phosphatidylinositol 3-kinase</subject><subject>Phosphatidylinositol 3-Kinases - antagonists & inhibitors</subject><subject>Phosphatidylinositol 3-Kinases - physiology</subject><subject>Protein kinase C</subject><subject>RAW 264.7 macrophages</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>wortmannin</subject><issn>0892-3973</issn><issn>1532-2513</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10M1v2jAYBnCr2tSytsdeq1y2W1p_xLFzjGiAaClBCWjayXpxHBUUSGuDpv738wQt2oHTe_m9jx49CN0R_ECwJI_5bBISjAkWjMUXaEA4oyHlhH1BAywTGrJEsCv0zbm1V4nA_BJdERInEaZigKazSVnPJuk8f_pd5NOyzudlEbDwZz5N6yyosvGiSOdZHcyqsshHWeVlOQ3KUVClvwIaRw8ieE6HVekzxll9g7620Dlze7zXaDHK5sNJWJTjfJgWoWaC7kItQNMEQwNL0fI2Ac2bVmjfKYpZRPWSxkYTCpwbwnWy5GC45A3XnEYxRJRdox-H3Ffbv-2N26nNymnTdbA1_d4pIlkUy0h6GB6gtr1z1rTq1a42YN8VwerfgMoPqD4H9P7-GLxfbkxz0sfFPPh-BOA0dK2FrV65k2NSCs-8kwe32ra93cCf3naN2sF719uPJ3aug_jv9cVAt3vRYI1a93u79cOeaf8XWF6VAg</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Kim, Hyung-Min</creator><creator>Yim, Hee-Gyun</creator><creator>Yoon, Hyang-Suk</creator><creator>Park, Seung-Taeck</creator><creator>Jeung, Jae-Yeal</creator><creator>Lee, Ki-Nam</creator><creator>Baek, Seung-Hwa</creator><creator>Song, Yung-Sun</creator><creator>Oh, Gyung-Jae</creator><creator>Kim, Nam-Song</creator><creator>An, Nyeon-Hyoung</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Dekker</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20010101</creationdate><title>PHOSPHATIDYLINOSITOL 3-KINASE REGULATES PROLIFERATION OF RAW 264.7 MACROPHAGES</title><author>Kim, Hyung-Min ; Yim, Hee-Gyun ; Yoon, Hyang-Suk ; Park, Seung-Taeck ; Jeung, Jae-Yeal ; Lee, Ki-Nam ; Baek, Seung-Hwa ; Song, Yung-Sun ; Oh, Gyung-Jae ; Kim, Nam-Song ; An, Nyeon-Hyoung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-c7ac290adab7f5f9ac5df7c94046342cb26ec12a55e15c9b5ae585d5c5246a423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Androstadienes - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>c-fos</topic><topic>c-jun</topic><topic>Cell Cycle - drug effects</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - physiology</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Chromones - pharmacology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - drug effects</topic><topic>Genes, fos - drug effects</topic><topic>Genes, jun - drug effects</topic><topic>Macrophages - cytology</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - enzymology</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Morpholines - pharmacology</topic><topic>phorbol 12-myristate 13-acetate</topic><topic>Phosphatidylinositol 3-kinase</topic><topic>Phosphatidylinositol 3-Kinases - antagonists & inhibitors</topic><topic>Phosphatidylinositol 3-Kinases - physiology</topic><topic>Protein kinase C</topic><topic>RAW 264.7 macrophages</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>wortmannin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hyung-Min</creatorcontrib><creatorcontrib>Yim, Hee-Gyun</creatorcontrib><creatorcontrib>Yoon, Hyang-Suk</creatorcontrib><creatorcontrib>Park, Seung-Taeck</creatorcontrib><creatorcontrib>Jeung, Jae-Yeal</creatorcontrib><creatorcontrib>Lee, Ki-Nam</creatorcontrib><creatorcontrib>Baek, Seung-Hwa</creatorcontrib><creatorcontrib>Song, Yung-Sun</creatorcontrib><creatorcontrib>Oh, Gyung-Jae</creatorcontrib><creatorcontrib>Kim, Nam-Song</creatorcontrib><creatorcontrib>An, Nyeon-Hyoung</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Immunopharmacology and immunotoxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hyung-Min</au><au>Yim, Hee-Gyun</au><au>Yoon, Hyang-Suk</au><au>Park, Seung-Taeck</au><au>Jeung, Jae-Yeal</au><au>Lee, Ki-Nam</au><au>Baek, Seung-Hwa</au><au>Song, Yung-Sun</au><au>Oh, Gyung-Jae</au><au>Kim, Nam-Song</au><au>An, Nyeon-Hyoung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PHOSPHATIDYLINOSITOL 3-KINASE REGULATES PROLIFERATION OF RAW 264.7 MACROPHAGES</atitle><jtitle>Immunopharmacology and immunotoxicology</jtitle><addtitle>Immunopharmacol Immunotoxicol</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>23</volume><issue>3</issue><spage>355</spage><epage>365</epage><pages>355-365</pages><issn>0892-3973</issn><eissn>1532-2513</eissn><coden>IITOEF</coden><abstract>Phosphatidylinositol 3-kinase (PI3-kinase) is an enzyme that acts as a direct biochemical link between a novel phosphatidylinositol pathway and a number of proteins containing intrinsic or associated kinase activities. Here we demonstrate that wortmannin, PI3-kinase inhibitor, decreases the proliferation of RAW 264.7 macrophages and that another structurally unrelated inhibitor of PI3-kinase, LY294002, also inhibits the proliferation. These results indicate a possible involvement of PI3-kinase in RAW 264.7 macrophages growth regulation. Wortmannin stimulation of RAW 264.7 macrophages is followed by sustained expression of the mRNA of c-fos and a transient expression of the mRNA of c-jun. We also show that the wortmannin and LY294002 induce a cell cycle arrest in asynchronously growing cells leading to an inhibition of cell proliferation after 12 h of treatment. In addition, wortmannin or LY294002 inhibited the phorbol 12-myristate 13-acetate-induced macrophages proliferation potently. These results suggest that PI3-kinase plays an important role in growth regulation of RAW 264.7 macrophages and that protein kinase C is a down stream effector of PI3-kinase.</abstract><cop>Monticello, NY</cop><pub>Informa UK Ltd</pub><pmid>11694027</pmid><doi>10.1081/IPH-100107336</doi><tpages>11</tpages></addata></record> |
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| ispartof | Immunopharmacology and immunotoxicology, 2001-01, Vol.23 (3), p.355-365 |
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| subjects | Androstadienes - pharmacology Animals Biological and medical sciences c-fos c-jun Cell Cycle - drug effects Cell cycle, cell proliferation Cell Division - drug effects Cell Division - physiology Cell Line Cell physiology Chromones - pharmacology Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression - drug effects Genes, fos - drug effects Genes, jun - drug effects Macrophages - cytology Macrophages - drug effects Macrophages - enzymology Mice Molecular and cellular biology Morpholines - pharmacology phorbol 12-myristate 13-acetate Phosphatidylinositol 3-kinase Phosphatidylinositol 3-Kinases - antagonists & inhibitors Phosphatidylinositol 3-Kinases - physiology Protein kinase C RAW 264.7 macrophages RNA, Messenger - genetics RNA, Messenger - metabolism Tetradecanoylphorbol Acetate - pharmacology wortmannin |
| title | PHOSPHATIDYLINOSITOL 3-KINASE REGULATES PROLIFERATION OF RAW 264.7 MACROPHAGES |
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