Improved DNA extraction from field soils for a highly sensitive quantification of Phomopsis sclerotioides by real-time PCR

For highly sensitive quantification of Phomopsis sclerotioides DNA from naturally infested watermelon field soils by real-time PCR, we improved a DNA extraction method and evaluated the extraction efficiency and the inhibitory effect of the soil extracts on the PCR. We used a FastDNA Spin Kit for So...

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Veröffentlicht in:	Japanese Journal of Phytopathology 2015, Vol.81(3), pp.194-203
Hauptverfasser:	NAKATA, N., YOKOYAMA, T., USHIO, S., NAGASAWA, T., YOSHIDA, S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Citrullus lanatus Cucumis sativus DNA extraction internal standard DNA Phomopsis Phomopsis sclerotioides quantification real-time PCR soil
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