High-intensity Exercise Modifies the Effects of Stanozolol on Brain Oxidative Stress in Rats
Abstract We analyzed the effects of high-intensity exercise (HIE) and anabolic androgenic steroids (AAS) on brain redox status. 40 male Wistar rats were randomly distributed in 4 experimental groups (n=10) with or without HIE and with or without weekly Stanozolol administration. Thiobarbituric acid-...
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| Veröffentlicht in: | International journal of sports medicine 2015-11, Vol.36 (12), p.984-991 |
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| creator | Camiletti-Moirón, D. Aparicio, V. A. Nebot, E. Medina, G. Martínez, R. Kapravelou, G. Andrade, A. Porres, J. M. López-Jurado, M. Aranda, P. |
| description | Abstract
We analyzed the effects of high-intensity exercise (HIE) and anabolic androgenic steroids (AAS) on brain redox status. 40 male Wistar rats were randomly distributed in 4 experimental groups (n=10) with or without HIE and with or without weekly Stanozolol administration. Thiobarbituric acid-reactive substances (TBARs) and protein carbonyl content (PCC) were assessed. Total superoxide dismutase (tSOD), manganese superoxide dismutase (Mn-SOD), copper/zinc superoxide dismutase (CuZn-SOD) and catalase (CAT) activities were measured. Finally, protein expression levels of glutathione peroxidase (GPx), NAD(P)H dehydrogenase, Quinone 1 (NQO1), NF-E2-Related Factor 2 (Nrf2), glial fibrillary acidic protein (GFAP), nuclear factor kappa β p65 (NF-κβ) and signal transducer and activator of transcription 3 were determined. Brain PCC concentrations were lower in the HIE groups compared to the untrained controls, whereas CAT activity was higher (both, p |
| doi_str_mv | 10.1055/s-0035-1548941 |
| format | Article |
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We analyzed the effects of high-intensity exercise (HIE) and anabolic androgenic steroids (AAS) on brain redox status. 40 male Wistar rats were randomly distributed in 4 experimental groups (n=10) with or without HIE and with or without weekly Stanozolol administration. Thiobarbituric acid-reactive substances (TBARs) and protein carbonyl content (PCC) were assessed. Total superoxide dismutase (tSOD), manganese superoxide dismutase (Mn-SOD), copper/zinc superoxide dismutase (CuZn-SOD) and catalase (CAT) activities were measured. Finally, protein expression levels of glutathione peroxidase (GPx), NAD(P)H dehydrogenase, Quinone 1 (NQO1), NF-E2-Related Factor 2 (Nrf2), glial fibrillary acidic protein (GFAP), nuclear factor kappa β p65 (NF-κβ) and signal transducer and activator of transcription 3 were determined. Brain PCC concentrations were lower in the HIE groups compared to the untrained controls, whereas CAT activity was higher (both, p<0.01). Both HIE and AAS groups exhibited higher expression levels of GFAP and GPx, but lower NQO1 levels (all, p<0.05). There were increased expression levels of NF-κβ in the AAS groups (p<0.01). In addition, there was increased expression of Nrf2 in the HIE groups (p<0.001). HIE*AAS interactions were found on TBARs content and GFAP expression, with HIE downregulating and upregulating AAS-mediated increases in TBARs and GFAP, respectively (p<0.05). Overall, HIE appeared to reduce the AAS-mediated negative effect on brain redox status.</description><identifier>ISSN: 0172-4622</identifier><identifier>EISSN: 1439-3964</identifier><identifier>DOI: 10.1055/s-0035-1548941</identifier><identifier>PMID: 26252547</identifier><language>eng</language><publisher>Stuttgart · New York: Georg Thieme Verlag KG</publisher><subject>Anabolic Agents - pharmacology ; Animals ; Biomarkers - metabolism ; Body Weight ; Brain - anatomy & histology ; Brain - enzymology ; Brain - metabolism ; Eating ; Male ; Organ Size ; Oxidative Stress - drug effects ; Physical Conditioning, Animal - methods ; Physiology & Biochemistry ; Protein Carbonylation ; Random Allocation ; Rats, Wistar ; Stanozolol - pharmacology ; Thiobarbituric Acid Reactive Substances - metabolism</subject><ispartof>International journal of sports medicine, 2015-11, Vol.36 (12), p.984-991</ispartof><rights>Georg Thieme Verlag KG Stuttgart · New York.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-d81b529621109fdcb815277b51e1cf0491cf706254c17daa3c24c0691df5d0a33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0035-1548941.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-0035-1548941$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,780,784,3017,3018,27924,27925,54559,54560</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26252547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Camiletti-Moirón, D.</creatorcontrib><creatorcontrib>Aparicio, V. A.</creatorcontrib><creatorcontrib>Nebot, E.</creatorcontrib><creatorcontrib>Medina, G.</creatorcontrib><creatorcontrib>Martínez, R.</creatorcontrib><creatorcontrib>Kapravelou, G.</creatorcontrib><creatorcontrib>Andrade, A.</creatorcontrib><creatorcontrib>Porres, J. M.</creatorcontrib><creatorcontrib>López-Jurado, M.</creatorcontrib><creatorcontrib>Aranda, P.</creatorcontrib><title>High-intensity Exercise Modifies the Effects of Stanozolol on Brain Oxidative Stress in Rats</title><title>International journal of sports medicine</title><addtitle>Int J Sports Med</addtitle><description>Abstract
We analyzed the effects of high-intensity exercise (HIE) and anabolic androgenic steroids (AAS) on brain redox status. 40 male Wistar rats were randomly distributed in 4 experimental groups (n=10) with or without HIE and with or without weekly Stanozolol administration. Thiobarbituric acid-reactive substances (TBARs) and protein carbonyl content (PCC) were assessed. Total superoxide dismutase (tSOD), manganese superoxide dismutase (Mn-SOD), copper/zinc superoxide dismutase (CuZn-SOD) and catalase (CAT) activities were measured. Finally, protein expression levels of glutathione peroxidase (GPx), NAD(P)H dehydrogenase, Quinone 1 (NQO1), NF-E2-Related Factor 2 (Nrf2), glial fibrillary acidic protein (GFAP), nuclear factor kappa β p65 (NF-κβ) and signal transducer and activator of transcription 3 were determined. Brain PCC concentrations were lower in the HIE groups compared to the untrained controls, whereas CAT activity was higher (both, p<0.01). Both HIE and AAS groups exhibited higher expression levels of GFAP and GPx, but lower NQO1 levels (all, p<0.05). There were increased expression levels of NF-κβ in the AAS groups (p<0.01). In addition, there was increased expression of Nrf2 in the HIE groups (p<0.001). HIE*AAS interactions were found on TBARs content and GFAP expression, with HIE downregulating and upregulating AAS-mediated increases in TBARs and GFAP, respectively (p<0.05). Overall, HIE appeared to reduce the AAS-mediated negative effect on brain redox status.</description><subject>Anabolic Agents - pharmacology</subject><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Body Weight</subject><subject>Brain - anatomy & histology</subject><subject>Brain - enzymology</subject><subject>Brain - metabolism</subject><subject>Eating</subject><subject>Male</subject><subject>Organ Size</subject><subject>Oxidative Stress - drug effects</subject><subject>Physical Conditioning, Animal - methods</subject><subject>Physiology & Biochemistry</subject><subject>Protein Carbonylation</subject><subject>Random Allocation</subject><subject>Rats, Wistar</subject><subject>Stanozolol - pharmacology</subject><subject>Thiobarbituric Acid Reactive Substances - metabolism</subject><issn>0172-4622</issn><issn>1439-3964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vEzEQhq0KREPh2mPlIxcHjz_24whVoEhFlaC9IVmOd9y42qyLx6lafj1bJXBDnEaaeebVq4exU5BLkNa-JyGltgKs6XoDR2wBRvdC9415wRYSWiVMo9Qxe010JyWYHvQrdqwaZZU17YL9uEi3G5GmihOl-sRXj1hCIuRf85BiQuJ1g3wVI4ZKPEf-vfop_8pjHnme-Mfi08SvHtPga3rA-VqQiM-7b77SG_Yy-pHw7WGesJtPq-vzC3F59fnL-YdLEXTTVDF0sLaqbxSA7OMQ1h1Y1bZrCwghyrlziK2cK5sA7eC9DsoE2fQwRDtIr_UJe7fPvS_55w6pum2igOPoJ8w7ctBppUwL2vwfbVWnbKcaOaPLPRpKJioY3X1JW1-eHEj3LN-Re5bvDvLnh7ND9m69xeEv_sf2DIg9UDcJt-ju8q5Ms5h_Bf4G2qCMGg</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Camiletti-Moirón, D.</creator><creator>Aparicio, V. A.</creator><creator>Nebot, E.</creator><creator>Medina, G.</creator><creator>Martínez, R.</creator><creator>Kapravelou, G.</creator><creator>Andrade, A.</creator><creator>Porres, J. M.</creator><creator>López-Jurado, M.</creator><creator>Aranda, P.</creator><general>Georg Thieme Verlag KG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TS</scope></search><sort><creationdate>20151101</creationdate><title>High-intensity Exercise Modifies the Effects of Stanozolol on Brain Oxidative Stress in Rats</title><author>Camiletti-Moirón, D. ; Aparicio, V. A. ; Nebot, E. ; Medina, G. ; Martínez, R. ; Kapravelou, G. ; Andrade, A. ; Porres, J. M. ; López-Jurado, M. ; Aranda, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-d81b529621109fdcb815277b51e1cf0491cf706254c17daa3c24c0691df5d0a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anabolic Agents - pharmacology</topic><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Body Weight</topic><topic>Brain - anatomy & histology</topic><topic>Brain - enzymology</topic><topic>Brain - metabolism</topic><topic>Eating</topic><topic>Male</topic><topic>Organ Size</topic><topic>Oxidative Stress - drug effects</topic><topic>Physical Conditioning, Animal - methods</topic><topic>Physiology & Biochemistry</topic><topic>Protein Carbonylation</topic><topic>Random Allocation</topic><topic>Rats, Wistar</topic><topic>Stanozolol - pharmacology</topic><topic>Thiobarbituric Acid Reactive Substances - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Camiletti-Moirón, D.</creatorcontrib><creatorcontrib>Aparicio, V. A.</creatorcontrib><creatorcontrib>Nebot, E.</creatorcontrib><creatorcontrib>Medina, G.</creatorcontrib><creatorcontrib>Martínez, R.</creatorcontrib><creatorcontrib>Kapravelou, G.</creatorcontrib><creatorcontrib>Andrade, A.</creatorcontrib><creatorcontrib>Porres, J. M.</creatorcontrib><creatorcontrib>López-Jurado, M.</creatorcontrib><creatorcontrib>Aranda, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Physical Education Index</collection><jtitle>International journal of sports medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Camiletti-Moirón, D.</au><au>Aparicio, V. A.</au><au>Nebot, E.</au><au>Medina, G.</au><au>Martínez, R.</au><au>Kapravelou, G.</au><au>Andrade, A.</au><au>Porres, J. M.</au><au>López-Jurado, M.</au><au>Aranda, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-intensity Exercise Modifies the Effects of Stanozolol on Brain Oxidative Stress in Rats</atitle><jtitle>International journal of sports medicine</jtitle><addtitle>Int J Sports Med</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>36</volume><issue>12</issue><spage>984</spage><epage>991</epage><pages>984-991</pages><issn>0172-4622</issn><eissn>1439-3964</eissn><abstract>Abstract
We analyzed the effects of high-intensity exercise (HIE) and anabolic androgenic steroids (AAS) on brain redox status. 40 male Wistar rats were randomly distributed in 4 experimental groups (n=10) with or without HIE and with or without weekly Stanozolol administration. Thiobarbituric acid-reactive substances (TBARs) and protein carbonyl content (PCC) were assessed. Total superoxide dismutase (tSOD), manganese superoxide dismutase (Mn-SOD), copper/zinc superoxide dismutase (CuZn-SOD) and catalase (CAT) activities were measured. Finally, protein expression levels of glutathione peroxidase (GPx), NAD(P)H dehydrogenase, Quinone 1 (NQO1), NF-E2-Related Factor 2 (Nrf2), glial fibrillary acidic protein (GFAP), nuclear factor kappa β p65 (NF-κβ) and signal transducer and activator of transcription 3 were determined. Brain PCC concentrations were lower in the HIE groups compared to the untrained controls, whereas CAT activity was higher (both, p<0.01). Both HIE and AAS groups exhibited higher expression levels of GFAP and GPx, but lower NQO1 levels (all, p<0.05). There were increased expression levels of NF-κβ in the AAS groups (p<0.01). In addition, there was increased expression of Nrf2 in the HIE groups (p<0.001). HIE*AAS interactions were found on TBARs content and GFAP expression, with HIE downregulating and upregulating AAS-mediated increases in TBARs and GFAP, respectively (p<0.05). Overall, HIE appeared to reduce the AAS-mediated negative effect on brain redox status.</abstract><cop>Stuttgart · New York</cop><pub>Georg Thieme Verlag KG</pub><pmid>26252547</pmid><doi>10.1055/s-0035-1548941</doi><tpages>8</tpages></addata></record> |
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| subjects | Anabolic Agents - pharmacology Animals Biomarkers - metabolism Body Weight Brain - anatomy & histology Brain - enzymology Brain - metabolism Eating Male Organ Size Oxidative Stress - drug effects Physical Conditioning, Animal - methods Physiology & Biochemistry Protein Carbonylation Random Allocation Rats, Wistar Stanozolol - pharmacology Thiobarbituric Acid Reactive Substances - metabolism |
| title | High-intensity Exercise Modifies the Effects of Stanozolol on Brain Oxidative Stress in Rats |
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