In Vitro High-Throughput Screening Assay for Modulators of Transcription

We developed a 96-well microtiter-plate high-throughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur...

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Veröffentlicht in:	Analytical biochemistry 2002-02, Vol.301 (1), p.103-110
Hauptverfasser:	Garcı́a-Martı́nez, León F., Bilter, Graham K., Wu, Jun, O'Neill, Joe, Barbosa, Miguel S., Kovelman, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Amanitins - pharmacology Animals Down-Regulation Drug Evaluation, Preclinical - methods high-throughput screening HTS Mammals Reproducibility of Results Ribonuclease, Pancreatic - metabolism RNA - analysis RNA - metabolism RNA Polymerase II - antagonists & inhibitors transcription Transcription, Genetic
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