AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.)
Rye (Secale cereale L.) is considered to be the most aluminum (Al)-tolerant species among the Triticeae. It has been suggested that aluminum tolerance in rye is controlled by three major genes (Alt genes) located on rye chromosome arms 3RL, 4RL, and 6RS, respectively. Screening of an [F.sub.6] rye r...
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| Veröffentlicht in: | Theoretical and applied genetics 2002-03, Vol.104 (4), p.626-631 |
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| description | Rye (Secale cereale L.) is considered to be the most aluminum (Al)-tolerant species among the Triticeae. It has been suggested that aluminum tolerance in rye is controlled by three major genes (Alt genes) located on rye chromosome arms 3RL, 4RL, and 6RS, respectively. Screening of an [F.sub.6] rye recombinant inbred line (RIL) population derived from the cross between an Al-tolerant rye (M39A-1-6) and an Al-sensitive rye (M77A-1) showed that a single gene controls aluminum tolerance in the population analyzed. In order to identify molecular markers tightly linked to the gene, we used a combination of amplified fragment length polymorphism (AFLP) and bulked segregant analysis techniques to evaluate the [F.sub.6] rye RIL population. We analyzed approximately 22,500 selectively amplified DNA fragments using 204 primer combinations and identified three AFLP markers tightly linked to the Alt gene. Two of these markers flanked the Alt locus at distance of 0.4 and 0.7 cM. Chromosomal localization using cloned AFLP and a restriction fragment length polymorphism (RFLP) marker indicated that the gene was on the long arm of rye chromosome 4R. The RFLP marker (BCD1230) co-segregated with the Alt gene. Since the gene is on chromosome 4R, the gene was designated as Alt3. These markers are being used as a starting point in the construction of a high resolution map of the Alt3 region in rye. |
| doi_str_mv | 10.1007/s00122-001-0782-3 |
| format | Article |
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J ; GUSTAFSON, J. P</creator><creatorcontrib>MIFTAHUDIN ; SCOLES, G. J ; GUSTAFSON, J. P</creatorcontrib><description>Rye (Secale cereale L.) is considered to be the most aluminum (Al)-tolerant species among the Triticeae. It has been suggested that aluminum tolerance in rye is controlled by three major genes (Alt genes) located on rye chromosome arms 3RL, 4RL, and 6RS, respectively. Screening of an [F.sub.6] rye recombinant inbred line (RIL) population derived from the cross between an Al-tolerant rye (M39A-1-6) and an Al-sensitive rye (M77A-1) showed that a single gene controls aluminum tolerance in the population analyzed. In order to identify molecular markers tightly linked to the gene, we used a combination of amplified fragment length polymorphism (AFLP) and bulked segregant analysis techniques to evaluate the [F.sub.6] rye RIL population. We analyzed approximately 22,500 selectively amplified DNA fragments using 204 primer combinations and identified three AFLP markers tightly linked to the Alt gene. Two of these markers flanked the Alt locus at distance of 0.4 and 0.7 cM. Chromosomal localization using cloned AFLP and a restriction fragment length polymorphism (RFLP) marker indicated that the gene was on the long arm of rye chromosome 4R. The RFLP marker (BCD1230) co-segregated with the Alt gene. Since the gene is on chromosome 4R, the gene was designated as Alt3. These markers are being used as a starting point in the construction of a high resolution map of the Alt3 region in rye.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-001-0782-3</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Adaptation to environment and cultivation conditions ; Agronomy. Soil science and plant productions ; Alt3 gene ; Aluminum ; Annealing ; Biological and medical sciences ; chromosome 3RL ; chromosome 4R ; chromosome 4RL ; chromosome 6RS ; Chromosomes ; Classical genetics, quantitative genetics, hybrids ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic aspects ; Genetics and breeding of economic plants ; Genetics of eukaryotes. Biological and molecular evolution ; Health aspects ; Physiological aspects ; Plant defenses ; Plant genetics ; Polymorphism ; Pteridophyta, spermatophyta ; Rye ; Secale cereale ; Seeds ; Varietal selection. Specialized plant breeding, plant breeding aims ; Vegetals</subject><ispartof>Theoretical and applied genetics, 2002-03, Vol.104 (4), p.626-631</ispartof><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2002 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-a22317c6543f5b09db5ccfe5bc48af8d9f6c6fb8af5c58b2d614ffc50ece22693</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13544261$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>MIFTAHUDIN</creatorcontrib><creatorcontrib>SCOLES, G. J</creatorcontrib><creatorcontrib>GUSTAFSON, J. P</creatorcontrib><title>AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.)</title><title>Theoretical and applied genetics</title><description>Rye (Secale cereale L.) is considered to be the most aluminum (Al)-tolerant species among the Triticeae. It has been suggested that aluminum tolerance in rye is controlled by three major genes (Alt genes) located on rye chromosome arms 3RL, 4RL, and 6RS, respectively. Screening of an [F.sub.6] rye recombinant inbred line (RIL) population derived from the cross between an Al-tolerant rye (M39A-1-6) and an Al-sensitive rye (M77A-1) showed that a single gene controls aluminum tolerance in the population analyzed. In order to identify molecular markers tightly linked to the gene, we used a combination of amplified fragment length polymorphism (AFLP) and bulked segregant analysis techniques to evaluate the [F.sub.6] rye RIL population. We analyzed approximately 22,500 selectively amplified DNA fragments using 204 primer combinations and identified three AFLP markers tightly linked to the Alt gene. Two of these markers flanked the Alt locus at distance of 0.4 and 0.7 cM. Chromosomal localization using cloned AFLP and a restriction fragment length polymorphism (RFLP) marker indicated that the gene was on the long arm of rye chromosome 4R. The RFLP marker (BCD1230) co-segregated with the Alt gene. Since the gene is on chromosome 4R, the gene was designated as Alt3. These markers are being used as a starting point in the construction of a high resolution map of the Alt3 region in rye.</description><subject>Adaptation to environment and cultivation conditions</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Alt3 gene</subject><subject>Aluminum</subject><subject>Annealing</subject><subject>Biological and medical sciences</subject><subject>chromosome 3RL</subject><subject>chromosome 4R</subject><subject>chromosome 4RL</subject><subject>chromosome 6RS</subject><subject>Chromosomes</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetics and breeding of economic plants</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Health aspects</subject><subject>Physiological aspects</subject><subject>Plant defenses</subject><subject>Plant genetics</subject><subject>Polymorphism</subject><subject>Pteridophyta, spermatophyta</subject><subject>Rye</subject><subject>Secale cereale</subject><subject>Seeds</subject><subject>Varietal selection. 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J ; GUSTAFSON, J. P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-a22317c6543f5b09db5ccfe5bc48af8d9f6c6fb8af5c58b2d614ffc50ece22693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adaptation to environment and cultivation conditions</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Alt3 gene</topic><topic>Aluminum</topic><topic>Annealing</topic><topic>Biological and medical sciences</topic><topic>chromosome 3RL</topic><topic>chromosome 4R</topic><topic>chromosome 4RL</topic><topic>chromosome 6RS</topic><topic>Chromosomes</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetics and breeding of economic plants</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Health aspects</topic><topic>Physiological aspects</topic><topic>Plant defenses</topic><topic>Plant genetics</topic><topic>Polymorphism</topic><topic>Pteridophyta, spermatophyta</topic><topic>Rye</topic><topic>Secale cereale</topic><topic>Seeds</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MIFTAHUDIN</creatorcontrib><creatorcontrib>SCOLES, G. J</creatorcontrib><creatorcontrib>GUSTAFSON, J. 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J</au><au>GUSTAFSON, J. P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.)</atitle><jtitle>Theoretical and applied genetics</jtitle><date>2002-03-01</date><risdate>2002</risdate><volume>104</volume><issue>4</issue><spage>626</spage><epage>631</epage><pages>626-631</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Rye (Secale cereale L.) is considered to be the most aluminum (Al)-tolerant species among the Triticeae. It has been suggested that aluminum tolerance in rye is controlled by three major genes (Alt genes) located on rye chromosome arms 3RL, 4RL, and 6RS, respectively. Screening of an [F.sub.6] rye recombinant inbred line (RIL) population derived from the cross between an Al-tolerant rye (M39A-1-6) and an Al-sensitive rye (M77A-1) showed that a single gene controls aluminum tolerance in the population analyzed. In order to identify molecular markers tightly linked to the gene, we used a combination of amplified fragment length polymorphism (AFLP) and bulked segregant analysis techniques to evaluate the [F.sub.6] rye RIL population. We analyzed approximately 22,500 selectively amplified DNA fragments using 204 primer combinations and identified three AFLP markers tightly linked to the Alt gene. Two of these markers flanked the Alt locus at distance of 0.4 and 0.7 cM. Chromosomal localization using cloned AFLP and a restriction fragment length polymorphism (RFLP) marker indicated that the gene was on the long arm of rye chromosome 4R. The RFLP marker (BCD1230) co-segregated with the Alt gene. Since the gene is on chromosome 4R, the gene was designated as Alt3. These markers are being used as a starting point in the construction of a high resolution map of the Alt3 region in rye.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s00122-001-0782-3</doi><tpages>6</tpages></addata></record> |
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| subjects | Adaptation to environment and cultivation conditions Agronomy. Soil science and plant productions Alt3 gene Aluminum Annealing Biological and medical sciences chromosome 3RL chromosome 4R chromosome 4RL chromosome 6RS Chromosomes Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology Genes Genetic aspects Genetics and breeding of economic plants Genetics of eukaryotes. Biological and molecular evolution Health aspects Physiological aspects Plant defenses Plant genetics Polymorphism Pteridophyta, spermatophyta Rye Secale cereale Seeds Varietal selection. Specialized plant breeding, plant breeding aims Vegetals |
| title | AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.) |
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