The amino acid requirements of Corynebacterium diphtheriae PW 8 substrain CN 2000
Aims: To determine the amino acid requirement and utilization pattern of Corynebacterium diphtheriae during growth and toxin production. Methods and Results: Comparing across different batches of beef‐based media, the growth and toxin yield were correlated significantly with nine of the amino acids....
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| Veröffentlicht in: | Journal of applied microbiology 2002-02, Vol.92 (2), p.215-220 |
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| creator | Nagarkar, P.P. Ravetkar, S.D. Watve, M.G. |
| description | Aims: To determine the amino acid requirement and utilization pattern of Corynebacterium diphtheriae during growth and toxin production.
Methods and Results: Comparing across different batches of beef‐based media, the growth and toxin yield were correlated significantly with nine of the amino acids. The amino acid utilization pattern during growth of C. diphtheriae further showed that only four of the nine amino acids, namely cystine, histidine, aspartate and methionine, were critical for growth of the vaccine strain. Further investigations using synthetic media with combinations of amino acid supplements demonstrated that among the four, cystine was the most growth limiting.
Conclusions: Only certain amino acids are critical for growth and toxin production by C. diphtheriae, cystine being the single most important.
Significance and Impact of the Study: Owing to the potential threat from Bovine Spongiform Encephalopathy (BSE), a need is recognized by vaccine manufacturers to substitute beef‐based production media. An understanding of the specific amino acid requirements would help to develop and optimize alternative production media. |
| doi_str_mv | 10.1046/j.1365-2672.2002.01521.x |
| format | Article |
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Methods and Results: Comparing across different batches of beef‐based media, the growth and toxin yield were correlated significantly with nine of the amino acids. The amino acid utilization pattern during growth of C. diphtheriae further showed that only four of the nine amino acids, namely cystine, histidine, aspartate and methionine, were critical for growth of the vaccine strain. Further investigations using synthetic media with combinations of amino acid supplements demonstrated that among the four, cystine was the most growth limiting.
Conclusions: Only certain amino acids are critical for growth and toxin production by C. diphtheriae, cystine being the single most important.
Significance and Impact of the Study: Owing to the potential threat from Bovine Spongiform Encephalopathy (BSE), a need is recognized by vaccine manufacturers to substitute beef‐based production media. An understanding of the specific amino acid requirements would help to develop and optimize alternative production media.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1046/j.1365-2672.2002.01521.x</identifier><identifier>PMID: 11849348</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Amino Acids - analysis ; Bacteriology ; Biological and medical sciences ; Biotechnology ; Corynebacterium diphtheriae ; Corynebacterium diphtheriae - growth & development ; Culture Media - chemistry ; Cystine - analysis ; Diphtheria Toxin - biosynthesis ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Growth, nutrition, cell differenciation ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Microbiology</subject><ispartof>Journal of applied microbiology, 2002-02, Vol.92 (2), p.215-220</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. Feb 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4531-2a6849e028a50577c78d2c7deccde254c786dc899c0a02a6fd94a2dfa15dbc813</citedby><cites>FETCH-LOGICAL-c4531-2a6849e028a50577c78d2c7deccde254c786dc899c0a02a6fd94a2dfa15dbc813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2672.2002.01521.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2672.2002.01521.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13949762$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11849348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagarkar, P.P.</creatorcontrib><creatorcontrib>Ravetkar, S.D.</creatorcontrib><creatorcontrib>Watve, M.G.</creatorcontrib><title>The amino acid requirements of Corynebacterium diphtheriae PW 8 substrain CN 2000</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: To determine the amino acid requirement and utilization pattern of Corynebacterium diphtheriae during growth and toxin production.
Methods and Results: Comparing across different batches of beef‐based media, the growth and toxin yield were correlated significantly with nine of the amino acids. The amino acid utilization pattern during growth of C. diphtheriae further showed that only four of the nine amino acids, namely cystine, histidine, aspartate and methionine, were critical for growth of the vaccine strain. Further investigations using synthetic media with combinations of amino acid supplements demonstrated that among the four, cystine was the most growth limiting.
Conclusions: Only certain amino acids are critical for growth and toxin production by C. diphtheriae, cystine being the single most important.
Significance and Impact of the Study: Owing to the potential threat from Bovine Spongiform Encephalopathy (BSE), a need is recognized by vaccine manufacturers to substitute beef‐based production media. An understanding of the specific amino acid requirements would help to develop and optimize alternative production media.</description><subject>Amino Acids - analysis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Corynebacterium diphtheriae</subject><subject>Corynebacterium diphtheriae - growth & development</subject><subject>Culture Media - chemistry</subject><subject>Cystine - analysis</subject><subject>Diphtheria Toxin - biosynthesis</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth, nutrition, cell differenciation</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Microbiology</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtKxDAUhoMojrdXkCDorjVJmzZduJDBK15hxGXIJKdMhl7GpMWZtzd1BgVXbpI_5DuHnw8hTElMSZqdz2OaZDxiWc5iRgiLCeWMxssttPfzsf2d04iTnI3QvvdzQmhCeLaLRpSKtEhSsYdeJzPAqrZNi5W2Bjv46K2DGprO47bE49atGpgq3YGzfY2NXcy6WcgK8Ms7Ftj3U985ZRs8fsKhCzlEO6WqPBxt7gP0dn01Gd9GD883d-PLh0inPKERU1noAIQJxQnPc50Lw3RuQGsDjKfhnRktikITRQJcmiJVzJSKcjPVgiYH6Gy9d-Hajx58J2vrNVSVaqDtvaSCCZoXIoAnf8B527smdJMsYUVGwxEgsYa0a713UMqFs7VyK0mJHJzLuRzUykGtHJzLb-dyGUaPN_v7aQ3md3AjOQCnG0B5rarSqUZb_8slRVrkGQvcxZr7tBWs_l1A3l8-Din5AnrJmts</recordid><startdate>200202</startdate><enddate>200202</enddate><creator>Nagarkar, P.P.</creator><creator>Ravetkar, S.D.</creator><creator>Watve, M.G.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200202</creationdate><title>The amino acid requirements of Corynebacterium diphtheriae PW 8 substrain CN 2000</title><author>Nagarkar, P.P. ; Ravetkar, S.D. ; Watve, M.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4531-2a6849e028a50577c78d2c7deccde254c786dc899c0a02a6fd94a2dfa15dbc813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acids - analysis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Corynebacterium diphtheriae</topic><topic>Corynebacterium diphtheriae - growth & development</topic><topic>Culture Media - chemistry</topic><topic>Cystine - analysis</topic><topic>Diphtheria Toxin - biosynthesis</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth, nutrition, cell differenciation</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagarkar, P.P.</creatorcontrib><creatorcontrib>Ravetkar, S.D.</creatorcontrib><creatorcontrib>Watve, M.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagarkar, P.P.</au><au>Ravetkar, S.D.</au><au>Watve, M.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The amino acid requirements of Corynebacterium diphtheriae PW 8 substrain CN 2000</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2002-02</date><risdate>2002</risdate><volume>92</volume><issue>2</issue><spage>215</spage><epage>220</epage><pages>215-220</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims: To determine the amino acid requirement and utilization pattern of Corynebacterium diphtheriae during growth and toxin production.
Methods and Results: Comparing across different batches of beef‐based media, the growth and toxin yield were correlated significantly with nine of the amino acids. The amino acid utilization pattern during growth of C. diphtheriae further showed that only four of the nine amino acids, namely cystine, histidine, aspartate and methionine, were critical for growth of the vaccine strain. Further investigations using synthetic media with combinations of amino acid supplements demonstrated that among the four, cystine was the most growth limiting.
Conclusions: Only certain amino acids are critical for growth and toxin production by C. diphtheriae, cystine being the single most important.
Significance and Impact of the Study: Owing to the potential threat from Bovine Spongiform Encephalopathy (BSE), a need is recognized by vaccine manufacturers to substitute beef‐based production media. An understanding of the specific amino acid requirements would help to develop and optimize alternative production media.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>11849348</pmid><doi>10.1046/j.1365-2672.2002.01521.x</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals |
| subjects | Amino Acids - analysis Bacteriology Biological and medical sciences Biotechnology Corynebacterium diphtheriae Corynebacterium diphtheriae - growth & development Culture Media - chemistry Cystine - analysis Diphtheria Toxin - biosynthesis Fermentation Fundamental and applied biological sciences. Psychology Growth, nutrition, cell differenciation Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Microbiology |
| title | The amino acid requirements of Corynebacterium diphtheriae PW 8 substrain CN 2000 |
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