Nile Red binding to HepG2 cells: An improved assay for in vitro studies of hepatosteatosis

Nile Red is a fluorescent dye used extensively to study fat accumulation in many types of cells; unfortunately protocols that work well for most cells are not effective for studying drug-induced lipid accumulation in cultured liver cells and hepatocyte-derived cell lines. Using human hepatoma (HepG2...

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Veröffentlicht in:	In vitro & molecular toxicology 2001, Vol.14 (3), p.177-190
Hauptverfasser:	MCMILLIAN, Michael K, GRANT, Elfrida R, ZHONG, Zhong, PARKER, J. Brandon, LI LI, ZIVIN, Robert A, BURCZYNSKI, Michael E, JOHNSON, Mark D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Survival Dogs ethionine Fatty Liver - chemically induced Fatty Liver - metabolism Fatty Liver - pathology Fluorescent Dyes Gastroenterology. Liver. Pancreas. Abdomen Hepatocytes - drug effects Hepatocytes - metabolism Hepatocytes - pathology Hepatosteatosis Humans In Vitro Techniques Indicators and Reagents - pharmacokinetics Lipids - analysis Liver. Biliary tract. Portal circulation. Exocrine pancreas Medical sciences Microscopy, Fluorescence Nile red Other diseases. Semiology Oxazines - pharmacokinetics Oxidation-Reduction Rats steatosis Steroids - toxicity Tumor Cells, Cultured Xanthenes
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