CRISPR-Cas-Assisted Multiplexing (CAM): Simple Same-Day Multi-Locus Engineering in Yeast

Demands on the industrial and academic yeast strain engineer have increased significantly in the era of synthetic biology. Installing complex biosynthetic pathways and combining point mutations are tedious and time‐consuming using traditional methods. With multiplex engineering tools, these tasks ca...

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Veröffentlicht in:	Journal of cellular physiology 2016-12, Vol.231 (12), p.2563-2569
Hauptverfasser:	Walter, Jessica M., Chandran, Sunil S., Horwitz, Andrew A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosynthetic Pathways - genetics Cloning CRISPR-Cas Systems - genetics Genetic Engineering Genetic Loci Reagents RNA, Guide, CRISPR-Cas Systems - genetics Saccharomyces cerevisiae - genetics Yeasts
Online-Zugang:	Volltext
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