Metatranscriptome analysis of active microbial communities in produced water samples from the Marcellus Shale
Controlling microbial activity is a primary concern during the management of the large volumes of wastewater (produced water) generated during high-volume hydraulic fracturing. In this study we analyzed the transcriptional activity (metatranscriptomes) of three produced water samples from the Marcel...
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| Veröffentlicht in: | Microbial ecology 2016-10, Vol.72 (3), p.571-581 |
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| description | Controlling microbial activity is a primary concern during the management of the large volumes of wastewater (produced water) generated during high-volume hydraulic fracturing. In this study we analyzed the transcriptional activity (metatranscriptomes) of three produced water samples from the Marcellus Shale. The goal of this study was to describe active metabolic pathways of industrial concern for produced water management and reuse, and to improve understanding of produced water microbial activity. Metatranscriptome analysis revealed active biofilm formation, sulfide production, and stress management mechanisms of the produced water microbial communities. Biofilm-formation and sulfate-reduction pathways were identified in all samples. Genes related to a diverse array of stress response mechanisms were also identified with implications for biocide efficacy. Additionally, active expression of a methanogenesis pathway was identified in a sample of produced water collected prior to holding pond storage. The active microbial community identified by metatranscriptome analysis was markedly different than the community composition as identified by 16S rRNA sequencing, highlighting the value of evaluating the active microbial fraction during assessments of produced water biofouling potential and evaluation of biocide application strategies. These results indicate biofouling and corrosive microbial processes are active in produced water and should be taken into consideration while designing produced water reuse strategies. |
| doi_str_mv | 10.1007/s00248-016-0811-z |
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In this study we analyzed the transcriptional activity (metatranscriptomes) of three produced water samples from the Marcellus Shale. The goal of this study was to describe active metabolic pathways of industrial concern for produced water management and reuse, and to improve understanding of produced water microbial activity. Metatranscriptome analysis revealed active biofilm formation, sulfide production, and stress management mechanisms of the produced water microbial communities. Biofilm-formation and sulfate-reduction pathways were identified in all samples. Genes related to a diverse array of stress response mechanisms were also identified with implications for biocide efficacy. Additionally, active expression of a methanogenesis pathway was identified in a sample of produced water collected prior to holding pond storage. The active microbial community identified by metatranscriptome analysis was markedly different than the community composition as identified by 16S rRNA sequencing, highlighting the value of evaluating the active microbial fraction during assessments of produced water biofouling potential and evaluation of biocide application strategies. These results indicate biofouling and corrosive microbial processes are active in produced water and should be taken into consideration while designing produced water reuse strategies.</description><identifier>ISSN: 0095-3628</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s00248-016-0811-z</identifier><identifier>PMID: 27457653</identifier><language>eng</language><publisher>New York: Springer Science + Business Media</publisher><subject>Alginates - metabolism ; Bacteria - classification ; Bacteria - genetics ; Bacteria - metabolism ; Base Sequence ; Biocides ; Biofilms ; Biofilms - growth & development ; Biofouling ; Biomedical and Life Sciences ; Community composition ; Disinfectants ; DNA, Bacterial ; Ecology ; ENVIRONMENTAL MICROBIOLOGY ; Gene Expression Regulation, Bacterial ; Geoecology/Natural Processes ; Glucuronic Acid - metabolism ; Hexuronic Acids - metabolism ; Hydraulic fracturing ; Industrial Microbiology ; Life Sciences ; Metabolic Networks and Pathways - genetics ; Methane - biosynthesis ; Methane - metabolism ; Methanogenesis ; Microbial activity ; Microbial Consortia - genetics ; Microbial Ecology ; Microbiology ; Nature Conservation ; Osmotic Pressure ; Oxidative Stress ; Pennsylvania ; RNA, Ribosomal, 16S - genetics ; Shales ; Sulfate reduction ; Sulfides - metabolism ; Transcriptome - genetics ; Waste Water - microbiology ; Water - metabolism ; Water analysis ; Water management ; Water Microbiology ; Water Purification ; Water Quality/Water Pollution ; Water reuse ; Water sampling</subject><ispartof>Microbial ecology, 2016-10, Vol.72 (3), p.571-581</ispartof><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-efa6e4f7c826af7bb528ba76f14502bed4f5b96bb5e2408008aad0ee046c288a3</citedby><cites>FETCH-LOGICAL-c427t-efa6e4f7c826af7bb528ba76f14502bed4f5b96bb5e2408008aad0ee046c288a3</cites><orcidid>0000-0003-3142-6090</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48723243$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48723243$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27457653$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vikram, Amit</creatorcontrib><creatorcontrib>Lipus, Daniel</creatorcontrib><creatorcontrib>Bibby, Kyle</creatorcontrib><title>Metatranscriptome analysis of active microbial communities in produced water samples from the Marcellus Shale</title><title>Microbial ecology</title><addtitle>Microb Ecol</addtitle><addtitle>Microb Ecol</addtitle><description>Controlling microbial activity is a primary concern during the management of the large volumes of wastewater (produced water) generated during high-volume hydraulic fracturing. In this study we analyzed the transcriptional activity (metatranscriptomes) of three produced water samples from the Marcellus Shale. The goal of this study was to describe active metabolic pathways of industrial concern for produced water management and reuse, and to improve understanding of produced water microbial activity. Metatranscriptome analysis revealed active biofilm formation, sulfide production, and stress management mechanisms of the produced water microbial communities. Biofilm-formation and sulfate-reduction pathways were identified in all samples. Genes related to a diverse array of stress response mechanisms were also identified with implications for biocide efficacy. Additionally, active expression of a methanogenesis pathway was identified in a sample of produced water collected prior to holding pond storage. The active microbial community identified by metatranscriptome analysis was markedly different than the community composition as identified by 16S rRNA sequencing, highlighting the value of evaluating the active microbial fraction during assessments of produced water biofouling potential and evaluation of biocide application strategies. These results indicate biofouling and corrosive microbial processes are active in produced water and should be taken into consideration while designing produced water reuse strategies.</description><subject>Alginates - metabolism</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Base Sequence</subject><subject>Biocides</subject><subject>Biofilms</subject><subject>Biofilms - growth & development</subject><subject>Biofouling</subject><subject>Biomedical and Life Sciences</subject><subject>Community composition</subject><subject>Disinfectants</subject><subject>DNA, Bacterial</subject><subject>Ecology</subject><subject>ENVIRONMENTAL MICROBIOLOGY</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Geoecology/Natural Processes</subject><subject>Glucuronic Acid - metabolism</subject><subject>Hexuronic Acids - metabolism</subject><subject>Hydraulic fracturing</subject><subject>Industrial Microbiology</subject><subject>Life Sciences</subject><subject>Metabolic Networks and Pathways - genetics</subject><subject>Methane - biosynthesis</subject><subject>Methane - metabolism</subject><subject>Methanogenesis</subject><subject>Microbial activity</subject><subject>Microbial Consortia - genetics</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Nature Conservation</subject><subject>Osmotic Pressure</subject><subject>Oxidative Stress</subject><subject>Pennsylvania</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Shales</subject><subject>Sulfate reduction</subject><subject>Sulfides - metabolism</subject><subject>Transcriptome - genetics</subject><subject>Waste Water - microbiology</subject><subject>Water - metabolism</subject><subject>Water analysis</subject><subject>Water management</subject><subject>Water Microbiology</subject><subject>Water Purification</subject><subject>Water Quality/Water Pollution</subject><subject>Water reuse</subject><subject>Water sampling</subject><issn>0095-3628</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkUtv1TAQhS1ERS-FH8ACZIkNm5TxI7bvElXlIbViAUjsokkyob6Kk4vtgNpfX1-lVIhFxWqkme-cGc1h7IWAUwFg3yYAqV0FwlTghKhuHrGN0EpWwunvj9kGYFtXykh3zJ6mtAMQ1kj1hB1Lq2trarVh4ZIy5ohT6qLf5zkQxwnH6-QTnweOXfa_iAffxbn1OPJuDmGZfPaUuJ_4Ps790lHPf2OmyBOG_VgmQ5wDz1fELzF2NI5L4l-ucKRn7GjAMdHzu3rCvr0__3r2sbr4_OHT2buLqtPS5ooGNKQH2zlpcLBtW0vXojWD0DXIlno91O3WlD5JDQ7AIfZABNp00jlUJ-zN6lvu-7lQyk3w6XAITjQvqRFO2q2CWun_QcEAKA0Fff0PupuXWL51oMRWOGuEKpRYqfKylCINzT76gPG6EdAcYmvW2JoSW3OIrbkpmld3zksbqL9X_MmpAHIFUhlNPyj-tfoB15eraJfyHO9NtbNSSa3ULR0-rho</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Vikram, Amit</creator><creator>Lipus, Daniel</creator><creator>Bibby, Kyle</creator><general>Springer Science + Business Media</general><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3142-6090</orcidid></search><sort><creationdate>20161001</creationdate><title>Metatranscriptome analysis of active microbial communities in produced water samples from the Marcellus Shale</title><author>Vikram, Amit ; Lipus, Daniel ; Bibby, Kyle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-efa6e4f7c826af7bb528ba76f14502bed4f5b96bb5e2408008aad0ee046c288a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Alginates - metabolism</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Base Sequence</topic><topic>Biocides</topic><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Biofouling</topic><topic>Biomedical and Life Sciences</topic><topic>Community composition</topic><topic>Disinfectants</topic><topic>DNA, Bacterial</topic><topic>Ecology</topic><topic>ENVIRONMENTAL MICROBIOLOGY</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Geoecology/Natural Processes</topic><topic>Glucuronic Acid - metabolism</topic><topic>Hexuronic Acids - metabolism</topic><topic>Hydraulic fracturing</topic><topic>Industrial Microbiology</topic><topic>Life Sciences</topic><topic>Metabolic Networks and Pathways - genetics</topic><topic>Methane - biosynthesis</topic><topic>Methane - metabolism</topic><topic>Methanogenesis</topic><topic>Microbial activity</topic><topic>Microbial Consortia - genetics</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Nature Conservation</topic><topic>Osmotic Pressure</topic><topic>Oxidative Stress</topic><topic>Pennsylvania</topic><topic>RNA, Ribosomal, 16S - 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Academic</collection><jtitle>Microbial ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vikram, Amit</au><au>Lipus, Daniel</au><au>Bibby, Kyle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metatranscriptome analysis of active microbial communities in produced water samples from the Marcellus Shale</atitle><jtitle>Microbial ecology</jtitle><stitle>Microb Ecol</stitle><addtitle>Microb Ecol</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>72</volume><issue>3</issue><spage>571</spage><epage>581</epage><pages>571-581</pages><issn>0095-3628</issn><eissn>1432-184X</eissn><abstract>Controlling microbial activity is a primary concern during the management of the large volumes of wastewater (produced water) generated during high-volume hydraulic fracturing. In this study we analyzed the transcriptional activity (metatranscriptomes) of three produced water samples from the Marcellus Shale. The goal of this study was to describe active metabolic pathways of industrial concern for produced water management and reuse, and to improve understanding of produced water microbial activity. Metatranscriptome analysis revealed active biofilm formation, sulfide production, and stress management mechanisms of the produced water microbial communities. Biofilm-formation and sulfate-reduction pathways were identified in all samples. Genes related to a diverse array of stress response mechanisms were also identified with implications for biocide efficacy. Additionally, active expression of a methanogenesis pathway was identified in a sample of produced water collected prior to holding pond storage. The active microbial community identified by metatranscriptome analysis was markedly different than the community composition as identified by 16S rRNA sequencing, highlighting the value of evaluating the active microbial fraction during assessments of produced water biofouling potential and evaluation of biocide application strategies. These results indicate biofouling and corrosive microbial processes are active in produced water and should be taken into consideration while designing produced water reuse strategies.</abstract><cop>New York</cop><pub>Springer Science + Business Media</pub><pmid>27457653</pmid><doi>10.1007/s00248-016-0811-z</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3142-6090</orcidid></addata></record> |
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| subjects | Alginates - metabolism Bacteria - classification Bacteria - genetics Bacteria - metabolism Base Sequence Biocides Biofilms Biofilms - growth & development Biofouling Biomedical and Life Sciences Community composition Disinfectants DNA, Bacterial Ecology ENVIRONMENTAL MICROBIOLOGY Gene Expression Regulation, Bacterial Geoecology/Natural Processes Glucuronic Acid - metabolism Hexuronic Acids - metabolism Hydraulic fracturing Industrial Microbiology Life Sciences Metabolic Networks and Pathways - genetics Methane - biosynthesis Methane - metabolism Methanogenesis Microbial activity Microbial Consortia - genetics Microbial Ecology Microbiology Nature Conservation Osmotic Pressure Oxidative Stress Pennsylvania RNA, Ribosomal, 16S - genetics Shales Sulfate reduction Sulfides - metabolism Transcriptome - genetics Waste Water - microbiology Water - metabolism Water analysis Water management Water Microbiology Water Purification Water Quality/Water Pollution Water reuse Water sampling |
| title | Metatranscriptome analysis of active microbial communities in produced water samples from the Marcellus Shale |
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