Bile canaliculi formation and biliary transport in 3D sandwich-cultured hepatocytes in dependence of the extracellular matrix composition

Primary human hepatocytes (PHH) are still considered as gold standard for investigation of in vitro metabolism and hepatotoxicity in pharmaceutical research. It has been shown that the three-dimensional (3D) cultivation of PHH in a sandwich configuration between two layers of extracellular matrix (E...

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Veröffentlicht in:	Archives of toxicology 2016-10, Vol.90 (10), p.2497-2511
Hauptverfasser:	Deharde, Daniela, Schneider, Christin, Hiller, Thomas, Fischer, Nicolas, Kegel, Victoria, Lübberstedt, Marc, Freyer, Nora, Hengstler, Jan G., Andersson, Tommy B., Seehofer, Daniel, Pratschke, Johann, Zeilinger, Katrin, Damm, Georg
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Aged, 80 and over Bile Bile - metabolism Bile Canaliculi - metabolism Biological Transport Biomedical and Life Sciences Biomedicine Cell Adhesion Cell culture Cell Polarity Cell Survival Cells, Cultured Collagen Collagen - chemistry Drug Combinations Environmental Health Extracellular matrix Extracellular Matrix - metabolism Female Hepatocytes - cytology Hepatocytes - metabolism Humans In vitro Systems Laminin - chemistry Liver Male Microscopy, Phase-Contrast Middle Aged Occupational Medicine/Industrial Medicine Pharmacology/Toxicology Primary Cell Culture - methods Proteoglycans - chemistry
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