Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T-seq

G&T-seq enables sequencing of DNA and mRNA from the same single cell, allowing the effects of genomic variation on transcription to be studied. It is compatible with any WGA method and so can be tailored to specific applications. Parallel sequencing of a single cell's genome and transcripto...

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Veröffentlicht in:	Nature protocols 2016-11, Vol.11 (11), p.2081-2103
Hauptverfasser:	Macaulay, Iain C, Teng, Mabel J, Haerty, Wilfried, Kumar, Parveen, Ponting, Chris P, Voet, Thierry
Format:	Artikel
Sprache:	eng
Schlagworte:	38 38/23 38/39 38/71 631/1647/514/2254 631/208/212 631/208/514/1948 631/337/2019 Analytical Chemistry Aneuploidy Animals Biological Techniques Cell Line Computational biology Computational Biology/Bioinformatics DNA sequencing Gene Expression Profiling - methods Genomics - methods Humans Identification and classification Innovations Life Sciences Messenger RNA Mice Microarrays Organic Chemistry Polymorphism, Single Nucleotide protocol Sequence Analysis, DNA - methods Sequence Analysis, RNA - methods Single-Cell Analysis - methods
Online-Zugang:	Volltext
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