Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model
The aim of the current study was to examine the protective effects and mechanisms of Ndfipl on neurocytes in an experimental in vitro Parkinson's disease model induced by MPP+. The cell model was developed with dominant negative expression and suppressed expression of Ndfipl by means of transie...
Gespeichert in:
| Veröffentlicht in: | Genetics and molecular research 2016-04, Vol.15 (2) |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | |
| container_title | Genetics and molecular research |
| container_volume | 15 |
| creator | Xing, L F Guo, H P Wang, D T Sun, L H Pan, S Y |
| description | The aim of the current study was to examine the protective effects and mechanisms of Ndfipl on neurocytes in an experimental in vitro Parkinson's disease model induced by MPP+. The cell model was developed with dominant negative expression and suppressed expression of Ndfipl by means of transient transfection of Ndfipl-dominant negative and -inhibitory vectors. In total, four different Ndfipl cell models were established. Different methods were used to analyze the cells. The MTT method was used to detect the effect of Ndfipl on the survival rate and apoptosis of the cells induced by MPP(+). We further studied the roles of Ndfipl in inhibiting MPP(+)-induced SH-SY5Y apoptosis, protection, and ubiquitination of SH-SY5Y cells. Our results showed that Ndfipl reduced apoptosis and improved cell survival rate, indicating that Ndfipl has a neuroprotective effect. Furthermore, we found that Ndfipl binds to Nedd4-1, and that increased expression of Ndfipl significantly reduced Itch expression. We also found that increased ubiquitination played a role in Ndfipl-mediated processes, and that Ndfipl and α-synuclein interact. Additionally, the expression of Ndfipl reduced expression of α-synuclein. In conclusion, Ndfipl plays a significant role in protecting SH-SY5Y cells in in vitro Parkinson's disease models. |
| doi_str_mv | 10.4238/gmr.15026963 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1827919033</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1789038839</sourcerecordid><originalsourceid>FETCH-LOGICAL-c362t-e067937c454de46be871ea0bd50586c4146f4d683827d01868b6d6e581624de13</originalsourceid><addsrcrecordid>eNqFkTtPxDAQhC0E4t1RI3dQEPAjsZ0SIV7SCU46KK6KnHgDhiQO3txJ_Hty4kB0NLtTfDNa7RByxNl5KqS5eGnjOc-YULmSG2SXK62STBm2-UfvkD3EN8ZElhq2TXaE5loKoXdJN41hgGrwS6BQ16NCajtHW6hebeexRRpq-uBq3zc0dHR2l8zm2ZxW0DTU9qEfAnqkvhtdq7n0Qwx0auO77zB0J0idR7AItA0OmgOyVdsG4XC998nzzfXT1V0yeby9v7qcJJVUYkiAKZ1LXaVZ6iBVJRjNwbLSZSwzqkp5qurUKSON0I5xo0ypnILMcCVGB5f75PQ7t4_hYwE4FK3H1c22g7DAgo_GnOdMyv9RbUbOGJmP6Nk3WsWAGKEu-uhbGz8LzopVGcVYRvFTxogfr5MXZQvuF_75vvwCpEWD0A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1789038839</pqid></control><display><type>article</type><title>Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Xing, L F ; Guo, H P ; Wang, D T ; Sun, L H ; Pan, S Y</creator><creatorcontrib>Xing, L F ; Guo, H P ; Wang, D T ; Sun, L H ; Pan, S Y</creatorcontrib><description>The aim of the current study was to examine the protective effects and mechanisms of Ndfipl on neurocytes in an experimental in vitro Parkinson's disease model induced by MPP+. The cell model was developed with dominant negative expression and suppressed expression of Ndfipl by means of transient transfection of Ndfipl-dominant negative and -inhibitory vectors. In total, four different Ndfipl cell models were established. Different methods were used to analyze the cells. The MTT method was used to detect the effect of Ndfipl on the survival rate and apoptosis of the cells induced by MPP(+). We further studied the roles of Ndfipl in inhibiting MPP(+)-induced SH-SY5Y apoptosis, protection, and ubiquitination of SH-SY5Y cells. Our results showed that Ndfipl reduced apoptosis and improved cell survival rate, indicating that Ndfipl has a neuroprotective effect. Furthermore, we found that Ndfipl binds to Nedd4-1, and that increased expression of Ndfipl significantly reduced Itch expression. We also found that increased ubiquitination played a role in Ndfipl-mediated processes, and that Ndfipl and α-synuclein interact. Additionally, the expression of Ndfipl reduced expression of α-synuclein. In conclusion, Ndfipl plays a significant role in protecting SH-SY5Y cells in in vitro Parkinson's disease models.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/gmr.15026963</identifier><identifier>PMID: 27173227</identifier><language>eng</language><publisher>Brazil</publisher><subject>1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - adverse effects ; alpha-Synuclein - biosynthesis ; alpha-Synuclein - genetics ; Apoptosis - drug effects ; Apoptosis - genetics ; Carrier Proteins - biosynthesis ; Cell Survival - drug effects ; Endosomal Sorting Complexes Required for Transport - genetics ; Endosomal Sorting Complexes Required for Transport - metabolism ; Gene Expression Regulation - drug effects ; HEK293 Cells ; Humans ; Membrane Proteins - biosynthesis ; MPTP Poisoning ; Nedd4 Ubiquitin Protein Ligases ; Neurons - metabolism ; Neurons - pathology ; Neuroprotective Agents ; Parkinson Disease, Secondary - chemically induced ; Parkinson Disease, Secondary - genetics ; Parkinson Disease, Secondary - pathology ; Reactive Oxygen Species - metabolism ; Ubiquitin-Protein Ligases - genetics ; Ubiquitin-Protein Ligases - metabolism</subject><ispartof>Genetics and molecular research, 2016-04, Vol.15 (2)</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-e067937c454de46be871ea0bd50586c4146f4d683827d01868b6d6e581624de13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27173227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xing, L F</creatorcontrib><creatorcontrib>Guo, H P</creatorcontrib><creatorcontrib>Wang, D T</creatorcontrib><creatorcontrib>Sun, L H</creatorcontrib><creatorcontrib>Pan, S Y</creatorcontrib><title>Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>The aim of the current study was to examine the protective effects and mechanisms of Ndfipl on neurocytes in an experimental in vitro Parkinson's disease model induced by MPP+. The cell model was developed with dominant negative expression and suppressed expression of Ndfipl by means of transient transfection of Ndfipl-dominant negative and -inhibitory vectors. In total, four different Ndfipl cell models were established. Different methods were used to analyze the cells. The MTT method was used to detect the effect of Ndfipl on the survival rate and apoptosis of the cells induced by MPP(+). We further studied the roles of Ndfipl in inhibiting MPP(+)-induced SH-SY5Y apoptosis, protection, and ubiquitination of SH-SY5Y cells. Our results showed that Ndfipl reduced apoptosis and improved cell survival rate, indicating that Ndfipl has a neuroprotective effect. Furthermore, we found that Ndfipl binds to Nedd4-1, and that increased expression of Ndfipl significantly reduced Itch expression. We also found that increased ubiquitination played a role in Ndfipl-mediated processes, and that Ndfipl and α-synuclein interact. Additionally, the expression of Ndfipl reduced expression of α-synuclein. In conclusion, Ndfipl plays a significant role in protecting SH-SY5Y cells in in vitro Parkinson's disease models.</description><subject>1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - adverse effects</subject><subject>alpha-Synuclein - biosynthesis</subject><subject>alpha-Synuclein - genetics</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - genetics</subject><subject>Carrier Proteins - biosynthesis</subject><subject>Cell Survival - drug effects</subject><subject>Endosomal Sorting Complexes Required for Transport - genetics</subject><subject>Endosomal Sorting Complexes Required for Transport - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Membrane Proteins - biosynthesis</subject><subject>MPTP Poisoning</subject><subject>Nedd4 Ubiquitin Protein Ligases</subject><subject>Neurons - metabolism</subject><subject>Neurons - pathology</subject><subject>Neuroprotective Agents</subject><subject>Parkinson Disease, Secondary - chemically induced</subject><subject>Parkinson Disease, Secondary - genetics</subject><subject>Parkinson Disease, Secondary - pathology</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Ubiquitin-Protein Ligases - genetics</subject><subject>Ubiquitin-Protein Ligases - metabolism</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtPxDAQhC0E4t1RI3dQEPAjsZ0SIV7SCU46KK6KnHgDhiQO3txJ_Hty4kB0NLtTfDNa7RByxNl5KqS5eGnjOc-YULmSG2SXK62STBm2-UfvkD3EN8ZElhq2TXaE5loKoXdJN41hgGrwS6BQ16NCajtHW6hebeexRRpq-uBq3zc0dHR2l8zm2ZxW0DTU9qEfAnqkvhtdq7n0Qwx0auO77zB0J0idR7AItA0OmgOyVdsG4XC998nzzfXT1V0yeby9v7qcJJVUYkiAKZ1LXaVZ6iBVJRjNwbLSZSwzqkp5qurUKSON0I5xo0ypnILMcCVGB5f75PQ7t4_hYwE4FK3H1c22g7DAgo_GnOdMyv9RbUbOGJmP6Nk3WsWAGKEu-uhbGz8LzopVGcVYRvFTxogfr5MXZQvuF_75vvwCpEWD0A</recordid><startdate>20160425</startdate><enddate>20160425</enddate><creator>Xing, L F</creator><creator>Guo, H P</creator><creator>Wang, D T</creator><creator>Sun, L H</creator><creator>Pan, S Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20160425</creationdate><title>Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model</title><author>Xing, L F ; Guo, H P ; Wang, D T ; Sun, L H ; Pan, S Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-e067937c454de46be871ea0bd50586c4146f4d683827d01868b6d6e581624de13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - adverse effects</topic><topic>alpha-Synuclein - biosynthesis</topic><topic>alpha-Synuclein - genetics</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - genetics</topic><topic>Carrier Proteins - biosynthesis</topic><topic>Cell Survival - drug effects</topic><topic>Endosomal Sorting Complexes Required for Transport - genetics</topic><topic>Endosomal Sorting Complexes Required for Transport - metabolism</topic><topic>Gene Expression Regulation - drug effects</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Membrane Proteins - biosynthesis</topic><topic>MPTP Poisoning</topic><topic>Nedd4 Ubiquitin Protein Ligases</topic><topic>Neurons - metabolism</topic><topic>Neurons - pathology</topic><topic>Neuroprotective Agents</topic><topic>Parkinson Disease, Secondary - chemically induced</topic><topic>Parkinson Disease, Secondary - genetics</topic><topic>Parkinson Disease, Secondary - pathology</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Ubiquitin-Protein Ligases - genetics</topic><topic>Ubiquitin-Protein Ligases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xing, L F</creatorcontrib><creatorcontrib>Guo, H P</creatorcontrib><creatorcontrib>Wang, D T</creatorcontrib><creatorcontrib>Sun, L H</creatorcontrib><creatorcontrib>Pan, S Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xing, L F</au><au>Guo, H P</au><au>Wang, D T</au><au>Sun, L H</au><au>Pan, S Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2016-04-25</date><risdate>2016</risdate><volume>15</volume><issue>2</issue><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>The aim of the current study was to examine the protective effects and mechanisms of Ndfipl on neurocytes in an experimental in vitro Parkinson's disease model induced by MPP+. The cell model was developed with dominant negative expression and suppressed expression of Ndfipl by means of transient transfection of Ndfipl-dominant negative and -inhibitory vectors. In total, four different Ndfipl cell models were established. Different methods were used to analyze the cells. The MTT method was used to detect the effect of Ndfipl on the survival rate and apoptosis of the cells induced by MPP(+). We further studied the roles of Ndfipl in inhibiting MPP(+)-induced SH-SY5Y apoptosis, protection, and ubiquitination of SH-SY5Y cells. Our results showed that Ndfipl reduced apoptosis and improved cell survival rate, indicating that Ndfipl has a neuroprotective effect. Furthermore, we found that Ndfipl binds to Nedd4-1, and that increased expression of Ndfipl significantly reduced Itch expression. We also found that increased ubiquitination played a role in Ndfipl-mediated processes, and that Ndfipl and α-synuclein interact. Additionally, the expression of Ndfipl reduced expression of α-synuclein. In conclusion, Ndfipl plays a significant role in protecting SH-SY5Y cells in in vitro Parkinson's disease models.</abstract><cop>Brazil</cop><pmid>27173227</pmid><doi>10.4238/gmr.15026963</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1676-5680 |
| ispartof | Genetics and molecular research, 2016-04, Vol.15 (2) |
| issn | 1676-5680 1676-5680 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1827919033 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - adverse effects alpha-Synuclein - biosynthesis alpha-Synuclein - genetics Apoptosis - drug effects Apoptosis - genetics Carrier Proteins - biosynthesis Cell Survival - drug effects Endosomal Sorting Complexes Required for Transport - genetics Endosomal Sorting Complexes Required for Transport - metabolism Gene Expression Regulation - drug effects HEK293 Cells Humans Membrane Proteins - biosynthesis MPTP Poisoning Nedd4 Ubiquitin Protein Ligases Neurons - metabolism Neurons - pathology Neuroprotective Agents Parkinson Disease, Secondary - chemically induced Parkinson Disease, Secondary - genetics Parkinson Disease, Secondary - pathology Reactive Oxygen Species - metabolism Ubiquitin-Protein Ligases - genetics Ubiquitin-Protein Ligases - metabolism |
| title | Protective effects and mechanisms of Ndfipl on SH-SY5Y cell apoptosis in an in vitro Parkinson's disease model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T13%3A37%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protective%20effects%20and%20mechanisms%20of%20Ndfipl%20on%20SH-SY5Y%20cell%20apoptosis%20in%20an%20in%20vitro%20Parkinson's%20disease%20model&rft.jtitle=Genetics%20and%20molecular%20research&rft.au=Xing,%20L%20F&rft.date=2016-04-25&rft.volume=15&rft.issue=2&rft.issn=1676-5680&rft.eissn=1676-5680&rft_id=info:doi/10.4238/gmr.15026963&rft_dat=%3Cproquest_cross%3E1789038839%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1789038839&rft_id=info:pmid/27173227&rfr_iscdi=true |