Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus

Fenneropenaeus penicillatus is one of the major economic shrimp species in China. In this study, 14 novel microsatellite loci were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphisms were tested in 30 individ...

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Veröffentlicht in:	Genetics and molecular research 2016-03, Vol.15 (1)
Hauptverfasser:	Li, B B, Li, Z B, Ning, Y F, Shangguan, J B, Huang, Y S, Yuan, Y, Mao, X Q
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Decapoda Genetic Loci - genetics Heterozygote Microsatellite Repeats - genetics Penaeidae - genetics Polymorphism, Genetic - genetics
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creator	Li, B B Li, Z B Ning, Y F Shangguan, J B Huang, Y S Yuan, Y Mao, X Q
description	Fenneropenaeus penicillatus is one of the major economic shrimp species in China. In this study, 14 novel microsatellite loci were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphisms were tested in 30 individuals from a single-wild population. The results showed that the number of alleles at each locus ranged from two to four, and the polymorphism information content varied from 0.314 to 0.692. The observed and expected heterozygosities ranged from 0.3343 to 0.6542 and from 0.3458 to 0.6657, respectively. Three loci deviated significantly from Hardy-Weinberg equilibrium after a Bonferroni correction was applied, while no deviations were detected in the other 11 loci. The new microsatellite loci identified in this study could be useful in future F. penicillatus population genetic, conservation research, population structure assessment, and linkage map construction studies.
doi_str_mv	10.4238/gmr.15017387
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In this study, 14 novel microsatellite loci were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphisms were tested in 30 individuals from a single-wild population. The results showed that the number of alleles at each locus ranged from two to four, and the polymorphism information content varied from 0.314 to 0.692. The observed and expected heterozygosities ranged from 0.3343 to 0.6542 and from 0.3458 to 0.6657, respectively. Three loci deviated significantly from Hardy-Weinberg equilibrium after a Bonferroni correction was applied, while no deviations were detected in the other 11 loci. The new microsatellite loci identified in this study could be useful in future F. penicillatus population genetic, conservation research, population structure assessment, and linkage map construction studies.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/gmr.15017387</identifier><identifier>PMID: 27050991</identifier><language>eng</language><publisher>Brazil</publisher><subject>Animals ; Decapoda ; Genetic Loci - genetics ; Heterozygote ; Microsatellite Repeats - genetics ; Penaeidae - genetics ; Polymorphism, Genetic - genetics</subject><ispartof>Genetics and molecular research, 2016-03, Vol.15 (1)</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27050991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, B B</creatorcontrib><creatorcontrib>Li, Z B</creatorcontrib><creatorcontrib>Ning, Y F</creatorcontrib><creatorcontrib>Shangguan, J B</creatorcontrib><creatorcontrib>Huang, Y S</creatorcontrib><creatorcontrib>Yuan, Y</creatorcontrib><creatorcontrib>Mao, X Q</creatorcontrib><title>Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>Fenneropenaeus penicillatus is one of the major economic shrimp species in China. In this study, 14 novel microsatellite loci were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphisms were tested in 30 individuals from a single-wild population. The results showed that the number of alleles at each locus ranged from two to four, and the polymorphism information content varied from 0.314 to 0.692. The observed and expected heterozygosities ranged from 0.3343 to 0.6542 and from 0.3458 to 0.6657, respectively. Three loci deviated significantly from Hardy-Weinberg equilibrium after a Bonferroni correction was applied, while no deviations were detected in the other 11 loci. 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subjects	Animals Decapoda Genetic Loci - genetics Heterozygote Microsatellite Repeats - genetics Penaeidae - genetics Polymorphism, Genetic - genetics
title	Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus
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