Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland
Bivalve mollusks bioaccumulate toxins via ingestion of toxic dinoflagellates. In this study, Crassostrea gigas was used to investigate the effects related to Prorocentrum lima exposure. Oysters were fed with three diets Isochrysis galbana (2 × 106 cell mL−1) control treatment; algal mix of I. galban...
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| Veröffentlicht in: | Marine environmental research 2016-09, Vol.120, p.93-102 |
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| creator | Romero-Geraldo, Reyna de Jesús García-Lagunas, Norma Hernández-Saavedra, Norma Yolanda |
| description | Bivalve mollusks bioaccumulate toxins via ingestion of toxic dinoflagellates. In this study, Crassostrea gigas was used to investigate the effects related to Prorocentrum lima exposure. Oysters were fed with three diets Isochrysis galbana (2 × 106 cell mL−1) control treatment; algal mix of I. galbana (2 × 106) and P. lima (3 × 103 cell mL−1); and P. lima alone (3 × 103 cell mL−1). Feeding behavior changes, histopathological alterations, and expression patterns changes of genes involved in cell cycle (p21, cafp55, p53), cytoskeleton (tub, act), and inflammatory process (casp1) were evaluated. Results indicated that the presence of diarrheic shellfish poisoning by P. lima cells decreased the clearance rate (p |
| doi_str_mv | 10.1016/j.marenvres.2016.07.011 |
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•Effects of Prorocentrum lima cells were assessed in oyster Crassostrea gigas.•Prorocentrum lima affected feeding and structural loss of digestive gland.•Dinoflagellate induced digestive gland modifications in gene expression pattern.•Physiologic, histopathologic, and genomics analyses useful to estimate bivalve health.</description><identifier>ISSN: 0141-1136</identifier><identifier>EISSN: 1879-0291</identifier><identifier>DOI: 10.1016/j.marenvres.2016.07.011</identifier><identifier>PMID: 27475522</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Bivalvia ; Cell cycle ; Crassostrea - physiology ; Crassostrea gigas ; Cytoskeleton ; Digestion ; Digestive gland ; Dinoflagellida - physiology ; Environmental Monitoring ; Feeding Behavior ; Gene Expression - physiology ; Haptophyta ; Inflammation ; Isochrysis galbana ; Marine ; Marine Toxins - toxicity ; Prorocentrum lima</subject><ispartof>Marine environmental research, 2016-09, Vol.120, p.93-102</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-a76536b9c7b244ce3b962e5e008271eb786f4fd3dbec1dba0120016024283ed43</citedby><cites>FETCH-LOGICAL-c404t-a76536b9c7b244ce3b962e5e008271eb786f4fd3dbec1dba0120016024283ed43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141113616301295$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27475522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Romero-Geraldo, Reyna de Jesús</creatorcontrib><creatorcontrib>García-Lagunas, Norma</creatorcontrib><creatorcontrib>Hernández-Saavedra, Norma Yolanda</creatorcontrib><title>Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland</title><title>Marine environmental research</title><addtitle>Mar Environ Res</addtitle><description>Bivalve mollusks bioaccumulate toxins via ingestion of toxic dinoflagellates. In this study, Crassostrea gigas was used to investigate the effects related to Prorocentrum lima exposure. Oysters were fed with three diets Isochrysis galbana (2 × 106 cell mL−1) control treatment; algal mix of I. galbana (2 × 106) and P. lima (3 × 103 cell mL−1); and P. lima alone (3 × 103 cell mL−1). Feeding behavior changes, histopathological alterations, and expression patterns changes of genes involved in cell cycle (p21, cafp55, p53), cytoskeleton (tub, act), and inflammatory process (casp1) were evaluated. Results indicated that the presence of diarrheic shellfish poisoning by P. lima cells decreased the clearance rate (p < 0.05), induced structural loss, significantly decreased tubule area of the digestive gland (p < 0.05), and up-regulated in expression all gene (p < 0.05), suggesting that toxic cells might trigger inflammatory tissue process, disturb cell cycle and cytoskeleton representing a risk to oysters integrity.
•Effects of Prorocentrum lima cells were assessed in oyster Crassostrea gigas.•Prorocentrum lima affected feeding and structural loss of digestive gland.•Dinoflagellate induced digestive gland modifications in gene expression pattern.•Physiologic, histopathologic, and genomics analyses useful to estimate bivalve health.</description><subject>Animals</subject><subject>Bivalvia</subject><subject>Cell cycle</subject><subject>Crassostrea - physiology</subject><subject>Crassostrea gigas</subject><subject>Cytoskeleton</subject><subject>Digestion</subject><subject>Digestive gland</subject><subject>Dinoflagellida - physiology</subject><subject>Environmental Monitoring</subject><subject>Feeding Behavior</subject><subject>Gene Expression - physiology</subject><subject>Haptophyta</subject><subject>Inflammation</subject><subject>Isochrysis galbana</subject><subject>Marine</subject><subject>Marine Toxins - toxicity</subject><subject>Prorocentrum lima</subject><issn>0141-1136</issn><issn>1879-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EokvhL4CPXBJmHCdOuFWrQpEqwQHOlmNPgldJvNjJCs78cbza0iucxhp982b8HmNvEEoEbN4dytlEWk6RUilyowRVAuITtsNWdQWIDp-yHaDEArFqrtiLlA4AUCusn7MroaSqayF27Pc-mpRCWiMZPvrRJE4_jyFtkfga-PqduPNLGCYz0jSZlfiXGGKwtKxxm_nkZ_Oe3_m0himM3pqJm8XxkRY66-Tzkg8Lp2EguyaenxfFkdLqT8THKeMv2bPBTIlePdRr9u3D7df9XXH_-eOn_c19YSXItTCqqaum76zqhZSWqr5rBNUE0AqF1Ku2GeTgKteTRdcbQAHZGhBStBU5WV2ztxfdYww_tnyBnn2y528tFLakMet02T8p_gdF6FrZNBlVF9TGkFKkQR9jdiX-0gj6HJY-6Mew9DksDUrnsPLk64clWz-Te5z7m04Gbi4AZVdOnqJO1tNiyfmY_dQu-H8u-QP75KyG</recordid><startdate>201609</startdate><enddate>201609</enddate><creator>Romero-Geraldo, Reyna de Jesús</creator><creator>García-Lagunas, Norma</creator><creator>Hernández-Saavedra, Norma Yolanda</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7ST</scope><scope>7TN</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>SOI</scope></search><sort><creationdate>201609</creationdate><title>Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland</title><author>Romero-Geraldo, Reyna de Jesús ; García-Lagunas, Norma ; Hernández-Saavedra, Norma Yolanda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-a76536b9c7b244ce3b962e5e008271eb786f4fd3dbec1dba0120016024283ed43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Bivalvia</topic><topic>Cell cycle</topic><topic>Crassostrea - physiology</topic><topic>Crassostrea gigas</topic><topic>Cytoskeleton</topic><topic>Digestion</topic><topic>Digestive gland</topic><topic>Dinoflagellida - physiology</topic><topic>Environmental Monitoring</topic><topic>Feeding Behavior</topic><topic>Gene Expression - physiology</topic><topic>Haptophyta</topic><topic>Inflammation</topic><topic>Isochrysis galbana</topic><topic>Marine</topic><topic>Marine Toxins - toxicity</topic><topic>Prorocentrum lima</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Romero-Geraldo, Reyna de Jesús</creatorcontrib><creatorcontrib>García-Lagunas, Norma</creatorcontrib><creatorcontrib>Hernández-Saavedra, Norma Yolanda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Environment Abstracts</collection><jtitle>Marine environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Romero-Geraldo, Reyna de Jesús</au><au>García-Lagunas, Norma</au><au>Hernández-Saavedra, Norma Yolanda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland</atitle><jtitle>Marine environmental research</jtitle><addtitle>Mar Environ Res</addtitle><date>2016-09</date><risdate>2016</risdate><volume>120</volume><spage>93</spage><epage>102</epage><pages>93-102</pages><issn>0141-1136</issn><eissn>1879-0291</eissn><abstract>Bivalve mollusks bioaccumulate toxins via ingestion of toxic dinoflagellates. In this study, Crassostrea gigas was used to investigate the effects related to Prorocentrum lima exposure. Oysters were fed with three diets Isochrysis galbana (2 × 106 cell mL−1) control treatment; algal mix of I. galbana (2 × 106) and P. lima (3 × 103 cell mL−1); and P. lima alone (3 × 103 cell mL−1). Feeding behavior changes, histopathological alterations, and expression patterns changes of genes involved in cell cycle (p21, cafp55, p53), cytoskeleton (tub, act), and inflammatory process (casp1) were evaluated. Results indicated that the presence of diarrheic shellfish poisoning by P. lima cells decreased the clearance rate (p < 0.05), induced structural loss, significantly decreased tubule area of the digestive gland (p < 0.05), and up-regulated in expression all gene (p < 0.05), suggesting that toxic cells might trigger inflammatory tissue process, disturb cell cycle and cytoskeleton representing a risk to oysters integrity.
•Effects of Prorocentrum lima cells were assessed in oyster Crassostrea gigas.•Prorocentrum lima affected feeding and structural loss of digestive gland.•Dinoflagellate induced digestive gland modifications in gene expression pattern.•Physiologic, histopathologic, and genomics analyses useful to estimate bivalve health.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>27475522</pmid><doi>10.1016/j.marenvres.2016.07.011</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Bivalvia Cell cycle Crassostrea - physiology Crassostrea gigas Cytoskeleton Digestion Digestive gland Dinoflagellida - physiology Environmental Monitoring Feeding Behavior Gene Expression - physiology Haptophyta Inflammation Isochrysis galbana Marine Marine Toxins - toxicity Prorocentrum lima |
| title | Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland |
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