Development of reverse transcription loop-mediated isothermal amplification assay as a simple detection method of Chrysanthemum stem necrosis virus in chrysanthemum and tomato

•We have developed a simple detection method of Chrysanthemum stem necrosis virus (CSNV).•A primer set designed from the genome sequences of CSNV worked most efficiently at 63°C.•The RT-LAMP assay using crude RNA could detect CSNV from chrysanthemum and tomato samples successfully. For a simple and...

Ausführliche Beschreibung