Effects of maternal folic acid supplementation on gene methylation and being small for gestational age
Background Being small for gestational age (SGA), a foetal growth abnormality, has a long‐lasting impact on childhood health. Its aetiology and underlying mechanisms are not well understood. Underlying epigenetic changes of imprinted genes have emerged as a potential pathological pathway because the...
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| creator | Qian, Y.-Y. Huang, X.-L. Liang, H. Zhang, Z.-F. Xu, J.-H. Chen, J.-P. Yuan, W. He, L. Wang, L. Miao, M.-H. Du, J. Li, D.-K. |
| description | Background
Being small for gestational age (SGA), a foetal growth abnormality, has a long‐lasting impact on childhood health. Its aetiology and underlying mechanisms are not well understood. Underlying epigenetic changes of imprinted genes have emerged as a potential pathological pathway because they may be associated with growth, including SGA. As a common methyl donor, folic acid (FA) is essential for DNA methylation, synthesis and repair, and FA supplementation is widely recommended for women planning pregnancy. The present study aimed to investigate the inter‐relationships among methylation levels of two imprinted genes [H19 differentially methylated regions (DMRs) and MEST DMRs], maternal FA supplementation and SGA.
Methods
We conducted a case–control study. Umbilical cord blood was taken from 39 SGA infants and 49 controls whose birth weights are appropriate for gestational age (AGA). DNA methylation levels of H19 and MEST DMRs were determined by an analysis of mass array quantitative methylation.
Results
Statistically significantly higher methylation levels were observed at sites 7.8, 9 and 17.18 of H19 (P = 0.030, 0.016 and 0.050, respectively) in the SGA infants compared to the AGA group. In addition, the association was stronger in male births where the mothers took FA around conception at six H19 sites (P = 0.004, 0.005, 0.048, 0.002, 0.021 and 0.005, respectively).
Conclusions
Methylation levels at H19 DMRs were higher in SGA infants compared to AGA controls. It appears that the association may be influenced by maternal peri‐conception FA supplementation and also be sex‐specific. |
| doi_str_mv | 10.1111/jhn.12369 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1827904673</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1817844201</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5649-208196c61cd0e6e8c8646848635b1fc72bb2abfabb40e161131169bc07749ec33</originalsourceid><addsrcrecordid>eNqNkV1rFDEUhoModq1e-Ack4I1eTJuTZJLMpSy1VUqVorh4E5LsyXbW-dhOZtD992adtheCYAgEwvM-nOQl5CWwE8jrdHvTnQAXqnpEFiBUWXCtV4_JglUlL4TRcESepbRljClg7Ck54poLpnm1IPEsRgxjon2krRtx6FxDY9_UgbpQr2madrsGW-xGN9Z9R_PeYIe0xfFm38x3rltTj3W3oal1zSE-ZCjNiaxzG3xOnkTXJHxxdx6Tr-_PviwvistP5x-W7y6LUCpZFZwZqFRQENYMFZpglFRGGiVKDzFo7j13PjrvJUNQAAJAVT4wrWWFQYhj8mb27ob-dsoz2LZOAZvGddhPyYLhumJS6f9BQRspOYOMvv4L3fbT4admiilmlMnU25kKQ5_SgNHuhrp1w94Cs4eebO7J_ukps6_ujJNvcf1A3heTgdMZ-Fk3uP-3yX68uLpXFnOiTiP-eki44YfNz9Wl_XZ1bj_L6-Xqu7m2K_EbmvOqtg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1817060868</pqid></control><display><type>article</type><title>Effects of maternal folic acid supplementation on gene methylation and being small for gestational age</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Qian, Y.-Y. ; Huang, X.-L. ; Liang, H. ; Zhang, Z.-F. ; Xu, J.-H. ; Chen, J.-P. ; Yuan, W. ; He, L. ; Wang, L. ; Miao, M.-H. ; Du, J. ; Li, D.-K.</creator><creatorcontrib>Qian, Y.-Y. ; Huang, X.-L. ; Liang, H. ; Zhang, Z.-F. ; Xu, J.-H. ; Chen, J.-P. ; Yuan, W. ; He, L. ; Wang, L. ; Miao, M.-H. ; Du, J. ; Li, D.-K.</creatorcontrib><description>Background
Being small for gestational age (SGA), a foetal growth abnormality, has a long‐lasting impact on childhood health. Its aetiology and underlying mechanisms are not well understood. Underlying epigenetic changes of imprinted genes have emerged as a potential pathological pathway because they may be associated with growth, including SGA. As a common methyl donor, folic acid (FA) is essential for DNA methylation, synthesis and repair, and FA supplementation is widely recommended for women planning pregnancy. The present study aimed to investigate the inter‐relationships among methylation levels of two imprinted genes [H19 differentially methylated regions (DMRs) and MEST DMRs], maternal FA supplementation and SGA.
Methods
We conducted a case–control study. Umbilical cord blood was taken from 39 SGA infants and 49 controls whose birth weights are appropriate for gestational age (AGA). DNA methylation levels of H19 and MEST DMRs were determined by an analysis of mass array quantitative methylation.
Results
Statistically significantly higher methylation levels were observed at sites 7.8, 9 and 17.18 of H19 (P = 0.030, 0.016 and 0.050, respectively) in the SGA infants compared to the AGA group. In addition, the association was stronger in male births where the mothers took FA around conception at six H19 sites (P = 0.004, 0.005, 0.048, 0.002, 0.021 and 0.005, respectively).
Conclusions
Methylation levels at H19 DMRs were higher in SGA infants compared to AGA controls. It appears that the association may be influenced by maternal peri‐conception FA supplementation and also be sex‐specific.</description><identifier>ISSN: 0952-3871</identifier><identifier>EISSN: 1365-277X</identifier><identifier>DOI: 10.1111/jhn.12369</identifier><identifier>PMID: 27230729</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adult ; birth weight ; Case-Control Studies ; China - epidemiology ; Cohort Studies ; Dietary Supplements ; DNA Methylation ; Epigenesis, Genetic ; epigenetics ; Female ; Fetal Blood - metabolism ; Fetal Development ; Fetal Growth Retardation - blood ; Fetal Growth Retardation - epidemiology ; Fetal Growth Retardation - metabolism ; Fetal Growth Retardation - prevention & control ; foetal growth ; folic acid ; Folic Acid - therapeutic use ; Genes ; Humans ; Infant, Newborn ; Infant, Small for Gestational Age ; Male ; Maternal Nutritional Physiological Phenomena ; Preconception Care ; Pregnancy ; Prenatal Care ; Proteins - genetics ; Proteins - metabolism ; Risk Factors ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Sex Factors</subject><ispartof>Journal of human nutrition and dietetics, 2016-10, Vol.29 (5), p.643-651</ispartof><rights>2016 The British Dietetic Association Ltd.</rights><rights>2016 The British Dietetic Association Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5649-208196c61cd0e6e8c8646848635b1fc72bb2abfabb40e161131169bc07749ec33</citedby><cites>FETCH-LOGICAL-c5649-208196c61cd0e6e8c8646848635b1fc72bb2abfabb40e161131169bc07749ec33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjhn.12369$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjhn.12369$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27230729$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qian, Y.-Y.</creatorcontrib><creatorcontrib>Huang, X.-L.</creatorcontrib><creatorcontrib>Liang, H.</creatorcontrib><creatorcontrib>Zhang, Z.-F.</creatorcontrib><creatorcontrib>Xu, J.-H.</creatorcontrib><creatorcontrib>Chen, J.-P.</creatorcontrib><creatorcontrib>Yuan, W.</creatorcontrib><creatorcontrib>He, L.</creatorcontrib><creatorcontrib>Wang, L.</creatorcontrib><creatorcontrib>Miao, M.-H.</creatorcontrib><creatorcontrib>Du, J.</creatorcontrib><creatorcontrib>Li, D.-K.</creatorcontrib><title>Effects of maternal folic acid supplementation on gene methylation and being small for gestational age</title><title>Journal of human nutrition and dietetics</title><addtitle>J Hum Nutr Diet</addtitle><description>Background
Being small for gestational age (SGA), a foetal growth abnormality, has a long‐lasting impact on childhood health. Its aetiology and underlying mechanisms are not well understood. Underlying epigenetic changes of imprinted genes have emerged as a potential pathological pathway because they may be associated with growth, including SGA. As a common methyl donor, folic acid (FA) is essential for DNA methylation, synthesis and repair, and FA supplementation is widely recommended for women planning pregnancy. The present study aimed to investigate the inter‐relationships among methylation levels of two imprinted genes [H19 differentially methylated regions (DMRs) and MEST DMRs], maternal FA supplementation and SGA.
Methods
We conducted a case–control study. Umbilical cord blood was taken from 39 SGA infants and 49 controls whose birth weights are appropriate for gestational age (AGA). DNA methylation levels of H19 and MEST DMRs were determined by an analysis of mass array quantitative methylation.
Results
Statistically significantly higher methylation levels were observed at sites 7.8, 9 and 17.18 of H19 (P = 0.030, 0.016 and 0.050, respectively) in the SGA infants compared to the AGA group. In addition, the association was stronger in male births where the mothers took FA around conception at six H19 sites (P = 0.004, 0.005, 0.048, 0.002, 0.021 and 0.005, respectively).
Conclusions
Methylation levels at H19 DMRs were higher in SGA infants compared to AGA controls. It appears that the association may be influenced by maternal peri‐conception FA supplementation and also be sex‐specific.</description><subject>Adult</subject><subject>birth weight</subject><subject>Case-Control Studies</subject><subject>China - epidemiology</subject><subject>Cohort Studies</subject><subject>Dietary Supplements</subject><subject>DNA Methylation</subject><subject>Epigenesis, Genetic</subject><subject>epigenetics</subject><subject>Female</subject><subject>Fetal Blood - metabolism</subject><subject>Fetal Development</subject><subject>Fetal Growth Retardation - blood</subject><subject>Fetal Growth Retardation - epidemiology</subject><subject>Fetal Growth Retardation - metabolism</subject><subject>Fetal Growth Retardation - prevention & control</subject><subject>foetal growth</subject><subject>folic acid</subject><subject>Folic Acid - therapeutic use</subject><subject>Genes</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Infant, Small for Gestational Age</subject><subject>Male</subject><subject>Maternal Nutritional Physiological Phenomena</subject><subject>Preconception Care</subject><subject>Pregnancy</subject><subject>Prenatal Care</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Risk Factors</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Sex Factors</subject><issn>0952-3871</issn><issn>1365-277X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1rFDEUhoModq1e-Ack4I1eTJuTZJLMpSy1VUqVorh4E5LsyXbW-dhOZtD992adtheCYAgEwvM-nOQl5CWwE8jrdHvTnQAXqnpEFiBUWXCtV4_JglUlL4TRcESepbRljClg7Ck54poLpnm1IPEsRgxjon2krRtx6FxDY9_UgbpQr2madrsGW-xGN9Z9R_PeYIe0xfFm38x3rltTj3W3oal1zSE-ZCjNiaxzG3xOnkTXJHxxdx6Tr-_PviwvistP5x-W7y6LUCpZFZwZqFRQENYMFZpglFRGGiVKDzFo7j13PjrvJUNQAAJAVT4wrWWFQYhj8mb27ob-dsoz2LZOAZvGddhPyYLhumJS6f9BQRspOYOMvv4L3fbT4admiilmlMnU25kKQ5_SgNHuhrp1w94Cs4eebO7J_ukps6_ujJNvcf1A3heTgdMZ-Fk3uP-3yX68uLpXFnOiTiP-eki44YfNz9Wl_XZ1bj_L6-Xqu7m2K_EbmvOqtg</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Qian, Y.-Y.</creator><creator>Huang, X.-L.</creator><creator>Liang, H.</creator><creator>Zhang, Z.-F.</creator><creator>Xu, J.-H.</creator><creator>Chen, J.-P.</creator><creator>Yuan, W.</creator><creator>He, L.</creator><creator>Wang, L.</creator><creator>Miao, M.-H.</creator><creator>Du, J.</creator><creator>Li, D.-K.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>201610</creationdate><title>Effects of maternal folic acid supplementation on gene methylation and being small for gestational age</title><author>Qian, Y.-Y. ; Huang, X.-L. ; Liang, H. ; Zhang, Z.-F. ; Xu, J.-H. ; Chen, J.-P. ; Yuan, W. ; He, L. ; Wang, L. ; Miao, M.-H. ; Du, J. ; Li, D.-K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5649-208196c61cd0e6e8c8646848635b1fc72bb2abfabb40e161131169bc07749ec33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>birth weight</topic><topic>Case-Control Studies</topic><topic>China - epidemiology</topic><topic>Cohort Studies</topic><topic>Dietary Supplements</topic><topic>DNA Methylation</topic><topic>Epigenesis, Genetic</topic><topic>epigenetics</topic><topic>Female</topic><topic>Fetal Blood - metabolism</topic><topic>Fetal Development</topic><topic>Fetal Growth Retardation - blood</topic><topic>Fetal Growth Retardation - epidemiology</topic><topic>Fetal Growth Retardation - metabolism</topic><topic>Fetal Growth Retardation - prevention & control</topic><topic>foetal growth</topic><topic>folic acid</topic><topic>Folic Acid - therapeutic use</topic><topic>Genes</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Infant, Small for Gestational Age</topic><topic>Male</topic><topic>Maternal Nutritional Physiological Phenomena</topic><topic>Preconception Care</topic><topic>Pregnancy</topic><topic>Prenatal Care</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Risk Factors</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Sex Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qian, Y.-Y.</creatorcontrib><creatorcontrib>Huang, X.-L.</creatorcontrib><creatorcontrib>Liang, H.</creatorcontrib><creatorcontrib>Zhang, Z.-F.</creatorcontrib><creatorcontrib>Xu, J.-H.</creatorcontrib><creatorcontrib>Chen, J.-P.</creatorcontrib><creatorcontrib>Yuan, W.</creatorcontrib><creatorcontrib>He, L.</creatorcontrib><creatorcontrib>Wang, L.</creatorcontrib><creatorcontrib>Miao, M.-H.</creatorcontrib><creatorcontrib>Du, J.</creatorcontrib><creatorcontrib>Li, D.-K.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of human nutrition and dietetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qian, Y.-Y.</au><au>Huang, X.-L.</au><au>Liang, H.</au><au>Zhang, Z.-F.</au><au>Xu, J.-H.</au><au>Chen, J.-P.</au><au>Yuan, W.</au><au>He, L.</au><au>Wang, L.</au><au>Miao, M.-H.</au><au>Du, J.</au><au>Li, D.-K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of maternal folic acid supplementation on gene methylation and being small for gestational age</atitle><jtitle>Journal of human nutrition and dietetics</jtitle><addtitle>J Hum Nutr Diet</addtitle><date>2016-10</date><risdate>2016</risdate><volume>29</volume><issue>5</issue><spage>643</spage><epage>651</epage><pages>643-651</pages><issn>0952-3871</issn><eissn>1365-277X</eissn><abstract>Background
Being small for gestational age (SGA), a foetal growth abnormality, has a long‐lasting impact on childhood health. Its aetiology and underlying mechanisms are not well understood. Underlying epigenetic changes of imprinted genes have emerged as a potential pathological pathway because they may be associated with growth, including SGA. As a common methyl donor, folic acid (FA) is essential for DNA methylation, synthesis and repair, and FA supplementation is widely recommended for women planning pregnancy. The present study aimed to investigate the inter‐relationships among methylation levels of two imprinted genes [H19 differentially methylated regions (DMRs) and MEST DMRs], maternal FA supplementation and SGA.
Methods
We conducted a case–control study. Umbilical cord blood was taken from 39 SGA infants and 49 controls whose birth weights are appropriate for gestational age (AGA). DNA methylation levels of H19 and MEST DMRs were determined by an analysis of mass array quantitative methylation.
Results
Statistically significantly higher methylation levels were observed at sites 7.8, 9 and 17.18 of H19 (P = 0.030, 0.016 and 0.050, respectively) in the SGA infants compared to the AGA group. In addition, the association was stronger in male births where the mothers took FA around conception at six H19 sites (P = 0.004, 0.005, 0.048, 0.002, 0.021 and 0.005, respectively).
Conclusions
Methylation levels at H19 DMRs were higher in SGA infants compared to AGA controls. It appears that the association may be influenced by maternal peri‐conception FA supplementation and also be sex‐specific.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>27230729</pmid><doi>10.1111/jhn.12369</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult birth weight Case-Control Studies China - epidemiology Cohort Studies Dietary Supplements DNA Methylation Epigenesis, Genetic epigenetics Female Fetal Blood - metabolism Fetal Development Fetal Growth Retardation - blood Fetal Growth Retardation - epidemiology Fetal Growth Retardation - metabolism Fetal Growth Retardation - prevention & control foetal growth folic acid Folic Acid - therapeutic use Genes Humans Infant, Newborn Infant, Small for Gestational Age Male Maternal Nutritional Physiological Phenomena Preconception Care Pregnancy Prenatal Care Proteins - genetics Proteins - metabolism Risk Factors RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Sex Factors |
| title | Effects of maternal folic acid supplementation on gene methylation and being small for gestational age |
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