Metabolic differences between white and brown fat from fasting rabbits at physiological temperature

It has been suggested that activated brown adipose tissue (BAT) shows increased glucose metabolic activity. However, less is known about metabolic activity of BAT under conditions of fasting and normal temperature. The aim of this study was to compare the possible differences in energetic metabolism...

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Veröffentlicht in:Journal of molecular endocrinology 2015-04, Vol.54 (2), p.105-113
Hauptverfasser: López-Ibarra, Z, Modrego, J, Valero-Muñoz, M, Rodríguez-Sierra, P, Zamorano-León, J J, González-Cantalapiedra, A, de las Heras, N, Ballesteros, S, Lahera, V, López-Farré, A J
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container_issue 2
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container_title Journal of molecular endocrinology
container_volume 54
creator López-Ibarra, Z
Modrego, J
Valero-Muñoz, M
Rodríguez-Sierra, P
Zamorano-León, J J
González-Cantalapiedra, A
de las Heras, N
Ballesteros, S
Lahera, V
López-Farré, A J
description It has been suggested that activated brown adipose tissue (BAT) shows increased glucose metabolic activity. However, less is known about metabolic activity of BAT under conditions of fasting and normal temperature. The aim of this study was to compare the possible differences in energetic metabolism between BAT and white adipose tissue (WAT) obtained from rabbits under the conditions of physiological temperature and 24 h after fasting conditions. The study was carried out on New Zealand rabbits (n=10) maintained for a period of 8 weeks at 23±2 °C. Food was removed 24 h before BAT and WAT were obtained. Protein expression levels of the glycolytic-related protein, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate dehydrogenase were higher in WAT than that in BAT. The expression level of carnitine palmitoyltransferase 1 (CPT1) and CPT2, two fatty acid mitochondrial transporters, and the fatty acid β-oxidation-related enzyme, acyl CoA dehydrogenase, was higher in BAT than in WAT. Cytosolic malate dehydrogenase expression and malate dehydrogenase activity were higher in WAT than in BAT. However, lactate dehydrogenase expression and lactate content were significantly higher in BAT than in WAT. In , this study for the first time, to our knowledge, has described how under fasting and normal temperature conditions rabbit BAT seems to use anaerobic metabolism to provide energetic fuel, as opposed to WAT, where the malate–aspartate shuttle and, therefore, the gluconeogenic pathway seem to be potentiated.
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However, less is known about metabolic activity of BAT under conditions of fasting and normal temperature. The aim of this study was to compare the possible differences in energetic metabolism between BAT and white adipose tissue (WAT) obtained from rabbits under the conditions of physiological temperature and 24 h after fasting conditions. The study was carried out on New Zealand rabbits (n=10) maintained for a period of 8 weeks at 23±2 °C. Food was removed 24 h before BAT and WAT were obtained. Protein expression levels of the glycolytic-related protein, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate dehydrogenase were higher in WAT than that in BAT. The expression level of carnitine palmitoyltransferase 1 (CPT1) and CPT2, two fatty acid mitochondrial transporters, and the fatty acid β-oxidation-related enzyme, acyl CoA dehydrogenase, was higher in BAT than in WAT. Cytosolic malate dehydrogenase expression and malate dehydrogenase activity were higher in WAT than in BAT. However, lactate dehydrogenase expression and lactate content were significantly higher in BAT than in WAT. 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However, lactate dehydrogenase expression and lactate content were significantly higher in BAT than in WAT. In , this study for the first time, to our knowledge, has described how under fasting and normal temperature conditions rabbit BAT seems to use anaerobic metabolism to provide energetic fuel, as opposed to WAT, where the malate–aspartate shuttle and, therefore, the gluconeogenic pathway seem to be potentiated.</abstract><cop>England</cop><pub>Bioscientifica Ltd</pub><pmid>25701828</pmid><doi>10.1530/JME-14-0255</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
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subjects Aconitate Hydratase - metabolism
Adipose Tissue, Brown - enzymology
Adipose Tissue, Brown - metabolism
Adipose Tissue, White - enzymology
Adipose Tissue, White - metabolism
Animals
Blotting, Western
Fasting
Fatty Acids - metabolism
Glucose - metabolism
Glycolysis
L-Lactate Dehydrogenase - metabolism
Lactic Acid - metabolism
Lipid Metabolism
Malate Dehydrogenase - metabolism
Mitochondrial Proteins - metabolism
Rabbits
Temperature
title Metabolic differences between white and brown fat from fasting rabbits at physiological temperature
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