Metabolic activity of sperm cells: correlation with sperm cell concentration, viability and motility in the rabbit
The resazurin reduction test (RRT) is a useful technique to assess the metabolic rate of sperm cells. RRT depends on the ability of metabolically active cells to reduce the non-fluorescent dye resazurin to the fluorescent resorufin. The aim of this study was to develop a vital fluorometric method to...
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| Veröffentlicht in: | Zygote (Cambridge) 2016-10, Vol.24 (5), p.707-713 |
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| creator | Sabés-Alsina, Maria Planell, Núria Gil, Sílvia Tallo-Parra, Oriol Maya-Soriano, Maria José Taberner, Ester Piles, Miriam Sabés, Manel Lopez-Bejar, Manel |
| description | The resazurin reduction test (RRT) is a useful technique to assess the metabolic rate of sperm cells. RRT depends on the ability of metabolically active cells to reduce the non-fluorescent dye resazurin to the fluorescent resorufin. The aim of this study was to develop a vital fluorometric method to evaluate metabolic activity of rabbit sperm cells. Twenty-five rabbit males were included in the study. Viability and morphology, motility and metabolic activity were evaluated using an eosin-nigrosin staining, a computer-assisted semen analysis (CASA) and the RRT, respectively. Spearman rank correlation analysis was used to determine the correlation between RRT and semen parameters. After evaluation, a concentration of 10 × 106 sperm cells/ml was selected for further experiments with RRT. No significant correlation was found between the RRT results and the motility parameters. However, after RRT a significant positive correlation between relative fluorescence units and the percentage of alive spermatozoa (r = 0.62; P = 0.001) and a negative one with the percentage of sperm cells with acrosomic abnormalities (r = −0.45; P < 0.05) were detected. The vital assessment of metabolic rate of sperm cells by RRT could provide more information about semen quality than other routine semen analysis, correlating with sperm viability and acrosome status information. |
| doi_str_mv | 10.1017/S0967199415000726 |
| format | Article |
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RRT depends on the ability of metabolically active cells to reduce the non-fluorescent dye resazurin to the fluorescent resorufin. The aim of this study was to develop a vital fluorometric method to evaluate metabolic activity of rabbit sperm cells. Twenty-five rabbit males were included in the study. Viability and morphology, motility and metabolic activity were evaluated using an eosin-nigrosin staining, a computer-assisted semen analysis (CASA) and the RRT, respectively. Spearman rank correlation analysis was used to determine the correlation between RRT and semen parameters. After evaluation, a concentration of 10 × 106 sperm cells/ml was selected for further experiments with RRT. No significant correlation was found between the RRT results and the motility parameters. However, after RRT a significant positive correlation between relative fluorescence units and the percentage of alive spermatozoa (r = 0.62; P = 0.001) and a negative one with the percentage of sperm cells with acrosomic abnormalities (r = −0.45; P < 0.05) were detected. The vital assessment of metabolic rate of sperm cells by RRT could provide more information about semen quality than other routine semen analysis, correlating with sperm viability and acrosome status information.</description><identifier>ISSN: 0967-1994</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199415000726</identifier><identifier>PMID: 26964875</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Acrosome Reaction ; Animals ; Cell Survival ; Correlation analysis ; Fluorometry - methods ; Male ; Oxazines - metabolism ; Rabbits ; Semen Analysis - methods ; Sperm Count ; Sperm Motility ; Spermatozoa - cytology ; Spermatozoa - metabolism ; Xanthenes - metabolism</subject><ispartof>Zygote (Cambridge), 2016-10, Vol.24 (5), p.707-713</ispartof><rights>Copyright © Cambridge University Press 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-3c2999e2f1d561541c388a4fd014c9e060283370cf2bb2673afd241f2bdeac5b3</citedby><cites>FETCH-LOGICAL-c406t-3c2999e2f1d561541c388a4fd014c9e060283370cf2bb2673afd241f2bdeac5b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0967199415000726/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,777,781,27905,27906,55609</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26964875$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabés-Alsina, Maria</creatorcontrib><creatorcontrib>Planell, Núria</creatorcontrib><creatorcontrib>Gil, Sílvia</creatorcontrib><creatorcontrib>Tallo-Parra, Oriol</creatorcontrib><creatorcontrib>Maya-Soriano, Maria José</creatorcontrib><creatorcontrib>Taberner, Ester</creatorcontrib><creatorcontrib>Piles, Miriam</creatorcontrib><creatorcontrib>Sabés, Manel</creatorcontrib><creatorcontrib>Lopez-Bejar, Manel</creatorcontrib><title>Metabolic activity of sperm cells: correlation with sperm cell concentration, viability and motility in the rabbit</title><title>Zygote (Cambridge)</title><addtitle>Zygote</addtitle><description>The resazurin reduction test (RRT) is a useful technique to assess the metabolic rate of sperm cells. RRT depends on the ability of metabolically active cells to reduce the non-fluorescent dye resazurin to the fluorescent resorufin. The aim of this study was to develop a vital fluorometric method to evaluate metabolic activity of rabbit sperm cells. Twenty-five rabbit males were included in the study. Viability and morphology, motility and metabolic activity were evaluated using an eosin-nigrosin staining, a computer-assisted semen analysis (CASA) and the RRT, respectively. Spearman rank correlation analysis was used to determine the correlation between RRT and semen parameters. After evaluation, a concentration of 10 × 106 sperm cells/ml was selected for further experiments with RRT. No significant correlation was found between the RRT results and the motility parameters. However, after RRT a significant positive correlation between relative fluorescence units and the percentage of alive spermatozoa (r = 0.62; P = 0.001) and a negative one with the percentage of sperm cells with acrosomic abnormalities (r = −0.45; P < 0.05) were detected. The vital assessment of metabolic rate of sperm cells by RRT could provide more information about semen quality than other routine semen analysis, correlating with sperm viability and acrosome status information.</description><subject>Acrosome Reaction</subject><subject>Animals</subject><subject>Cell Survival</subject><subject>Correlation analysis</subject><subject>Fluorometry - methods</subject><subject>Male</subject><subject>Oxazines - metabolism</subject><subject>Rabbits</subject><subject>Semen Analysis - methods</subject><subject>Sperm Count</subject><subject>Sperm Motility</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - metabolism</subject><subject>Xanthenes - metabolism</subject><issn>0967-1994</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1LHTEUhoO06K3tD3AjgW666NhzkkwycSfSL7B00XY9JJmMRmYm1yTX4r93pvdWRCl0FQ7Pc94kvIQcIZwgoPrwA7RUqLXAGgAUk3tkhULqqlEcXpDVgquFH5BXOV8vjtJinxwwqaVoVL0i6ZsvxsYhOGpcCbeh3NHY07z2aaTOD0M-pS6m5AdTQpzo71CuHtGZTc5PJf2h7-ltMDYMS4iZOjrGsh3CRMuVp8lYG8pr8rI3Q_Zvduch-fXp48_zL9XF989fz88uKidAloo7prX2rMeullgLdLxpjOg7QOG0Bwms4VyB65m1TCpu-o4JnKfOG1dbfkjebXPXKd5sfC7tGPLyaDP5uMktNkxpYJw3_6MyRAYMZvXtE_U6btI0f2SxEKSsdT1buLVcijkn37frFEaT7lqEdumufdbdvHO8S97Y0XcPG3_LmgW-CzWjTaG79I_u_mfsPY9Eo4M</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Sabés-Alsina, Maria</creator><creator>Planell, Núria</creator><creator>Gil, Sílvia</creator><creator>Tallo-Parra, Oriol</creator><creator>Maya-Soriano, Maria José</creator><creator>Taberner, Ester</creator><creator>Piles, Miriam</creator><creator>Sabés, Manel</creator><creator>Lopez-Bejar, Manel</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201610</creationdate><title>Metabolic activity of sperm cells: correlation with sperm cell concentration, viability and motility in the rabbit</title><author>Sabés-Alsina, Maria ; 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RRT depends on the ability of metabolically active cells to reduce the non-fluorescent dye resazurin to the fluorescent resorufin. The aim of this study was to develop a vital fluorometric method to evaluate metabolic activity of rabbit sperm cells. Twenty-five rabbit males were included in the study. Viability and morphology, motility and metabolic activity were evaluated using an eosin-nigrosin staining, a computer-assisted semen analysis (CASA) and the RRT, respectively. Spearman rank correlation analysis was used to determine the correlation between RRT and semen parameters. After evaluation, a concentration of 10 × 106 sperm cells/ml was selected for further experiments with RRT. No significant correlation was found between the RRT results and the motility parameters. However, after RRT a significant positive correlation between relative fluorescence units and the percentage of alive spermatozoa (r = 0.62; P = 0.001) and a negative one with the percentage of sperm cells with acrosomic abnormalities (r = −0.45; P < 0.05) were detected. The vital assessment of metabolic rate of sperm cells by RRT could provide more information about semen quality than other routine semen analysis, correlating with sperm viability and acrosome status information.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>26964875</pmid><doi>10.1017/S0967199415000726</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Zygote (Cambridge), 2016-10, Vol.24 (5), p.707-713 |
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| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Acrosome Reaction Animals Cell Survival Correlation analysis Fluorometry - methods Male Oxazines - metabolism Rabbits Semen Analysis - methods Sperm Count Sperm Motility Spermatozoa - cytology Spermatozoa - metabolism Xanthenes - metabolism |
| title | Metabolic activity of sperm cells: correlation with sperm cell concentration, viability and motility in the rabbit |
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