Design and validation of small interfering RNA on respiratory syncytial virus M2-2 gene: A potential approach in RNA interference on viral replication

•Design and validation of siRNAs targeting RSV M2-2 gene.•SiRNAs efficiently silenced RSV M2-2 gene at both protein and gene level.•Designed siRNAs targeting RSV M2-2 gene produced inhibitory effects (>80%) at 50nM. Human respiratory syncytial virus (RSV) is the leading cause of severe lower resp...

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Veröffentlicht in:	Journal of virological methods 2016-10, Vol.236, p.117-125
Hauptverfasser:	Chin, V.k., Atika Aziz, Nur A., Hudu, Shuaibu A., Harmal, Nabil S., Syahrilnizam, A., Jalilian, Farid A., Zamberi, S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antiviral Antiviral Agents - metabolism Cercopithecus aethiops Flow Cytometry Fluorometry Gene silencing Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics Real-Time Polymerase Chain Reaction Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - genetics Respiratory syncytial virus Respiratory Syncytial Virus, Human - drug effects Respiratory Syncytial Virus, Human - physiology Reverse Transcriptase Polymerase Chain Reaction RNA Interference RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Small interfering RNA Vero Cells Viral Proteins - antagonists & inhibitors Viral Proteins - genetics Virus Replication - drug effects
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