Phenotypic and genotypic characterization of enteroaggregative Escherichia coli isolates from pediatric population in Pakistan
Enteroaggregative Escherichia coli (EAEC) are a leading cause of diarrhea among children. The objective of this study was to define the frequency of EAEC among diarrheal children from flood‐affected areas as well as sporadic cases, determine multidrug resistance, and evaluation of virulence using an...
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| Veröffentlicht in: | APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2016-10, Vol.124 (10), p.872-880 |
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| creator | Khalil, Uzma Younus, Mahwish Asghar, Naeem Siddiqui, Fariha Gómez-Duarte, Oscar G. Wren, Brendan W. Bokhari, Habib |
| description | Enteroaggregative Escherichia coli (EAEC) are a leading cause of diarrhea among children. The objective of this study was to define the frequency of EAEC among diarrheal children from flood‐affected areas as well as sporadic cases, determine multidrug resistance, and evaluation of virulence using an in vivo model of pathogenesis. Stool samples were collected from 225 diarrheal children from 2010 to 2011 from flood‐affected areas as well as from sporadic cases in Pakistan. Identified EAEC isolates were characterized by phylogrouping, antibiotic resistance patterns including the extended‐spectrum beta lactamase spectrum, single nucleotide polymorphism detection in gyrA and parC, and virulence potential using wax worm, G. mellonella. A total of 35 (12.5%) confirmed EAEC isolates were identified among 225 E. coli isolates. EAEC isolates displayed high resistance to tetracycline, ampicillin, and cefaclor. A total of 34.28% were ESBL positive. Single nucleotide polymorphism detection revealed 37.14% and 68.57% isolates were positive for SNPs in gyrA (A660‐T660) and parC (C330‐T330), respectively. Phylogrouping revealed that B2 phylogroup was more prevalent among all EAEC isolates tested followed by D, A, B1, and non‐typeable (NT). Infection of G. mellonella with EAEC showed that killing infective dose was 100% higher than E. coli DH5 alpha control. EAEC are prevalent among Pakistani children with diarrhea, they are highly resistant to antibiotics, and predominantly fall into B2 phylogroup. Epidemiologic surveillance of EAEC and other E. coli pathotypes is critical to assess not only the role of these pathogens in diarrheal disease but also to determine the extent of multidrug resistance among the population. |
| doi_str_mv | 10.1111/apm.12577 |
| format | Article |
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The objective of this study was to define the frequency of EAEC among diarrheal children from flood‐affected areas as well as sporadic cases, determine multidrug resistance, and evaluation of virulence using an in vivo model of pathogenesis. Stool samples were collected from 225 diarrheal children from 2010 to 2011 from flood‐affected areas as well as from sporadic cases in Pakistan. Identified EAEC isolates were characterized by phylogrouping, antibiotic resistance patterns including the extended‐spectrum beta lactamase spectrum, single nucleotide polymorphism detection in gyrA and parC, and virulence potential using wax worm, G. mellonella. A total of 35 (12.5%) confirmed EAEC isolates were identified among 225 E. coli isolates. EAEC isolates displayed high resistance to tetracycline, ampicillin, and cefaclor. A total of 34.28% were ESBL positive. Single nucleotide polymorphism detection revealed 37.14% and 68.57% isolates were positive for SNPs in gyrA (A660‐T660) and parC (C330‐T330), respectively. Phylogrouping revealed that B2 phylogroup was more prevalent among all EAEC isolates tested followed by D, A, B1, and non‐typeable (NT). Infection of G. mellonella with EAEC showed that killing infective dose was 100% higher than E. coli DH5 alpha control. EAEC are prevalent among Pakistani children with diarrhea, they are highly resistant to antibiotics, and predominantly fall into B2 phylogroup. Epidemiologic surveillance of EAEC and other E. coli pathotypes is critical to assess not only the role of these pathogens in diarrheal disease but also to determine the extent of multidrug resistance among the population.</description><identifier>ISSN: 0903-4641</identifier><identifier>EISSN: 1600-0463</identifier><identifier>DOI: 10.1111/apm.12577</identifier><identifier>PMID: 27485156</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Antibiotics ; beta-Lactamases - genetics ; Biological Assay ; Child, Preschool ; Diarrhea - epidemiology ; Diarrhea - microbiology ; Disease Models, Animal ; DNA Gyrase - genetics ; DNA Topoisomerase IV - genetics ; Drug Resistance, Bacterial ; E coli ; Enteroaggregative Escherichia coli ; Escherichia coli ; Escherichia coli - classification ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli - physiology ; Escherichia coli Infections - epidemiology ; Escherichia coli Infections - microbiology ; extended-spectrum beta lactamases ; Feces - microbiology ; Female ; Genes ; Genotype ; Humans ; Infant ; Infant, Newborn ; Lepidoptera ; Male ; Pakistan - epidemiology ; Phenotype ; phylogroups ; Polymorphism, Single Nucleotide ; single nucleotide polymorphism ; Survival Analysis ; Virulence ; Virulence Factors - analysis ; Virulence Factors - genetics ; virulence potential</subject><ispartof>APMIS : acta pathologica, microbiologica et immunologica Scandinavica, 2016-10, Vol.124 (10), p.872-880</ispartof><rights>2016 APMIS. 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The objective of this study was to define the frequency of EAEC among diarrheal children from flood‐affected areas as well as sporadic cases, determine multidrug resistance, and evaluation of virulence using an in vivo model of pathogenesis. Stool samples were collected from 225 diarrheal children from 2010 to 2011 from flood‐affected areas as well as from sporadic cases in Pakistan. Identified EAEC isolates were characterized by phylogrouping, antibiotic resistance patterns including the extended‐spectrum beta lactamase spectrum, single nucleotide polymorphism detection in gyrA and parC, and virulence potential using wax worm, G. mellonella. A total of 35 (12.5%) confirmed EAEC isolates were identified among 225 E. coli isolates. EAEC isolates displayed high resistance to tetracycline, ampicillin, and cefaclor. A total of 34.28% were ESBL positive. Single nucleotide polymorphism detection revealed 37.14% and 68.57% isolates were positive for SNPs in gyrA (A660‐T660) and parC (C330‐T330), respectively. Phylogrouping revealed that B2 phylogroup was more prevalent among all EAEC isolates tested followed by D, A, B1, and non‐typeable (NT). Infection of G. mellonella with EAEC showed that killing infective dose was 100% higher than E. coli DH5 alpha control. EAEC are prevalent among Pakistani children with diarrhea, they are highly resistant to antibiotics, and predominantly fall into B2 phylogroup. Epidemiologic surveillance of EAEC and other E. coli pathotypes is critical to assess not only the role of these pathogens in diarrheal disease but also to determine the extent of multidrug resistance among the population.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>beta-Lactamases - genetics</subject><subject>Biological Assay</subject><subject>Child, Preschool</subject><subject>Diarrhea - epidemiology</subject><subject>Diarrhea - microbiology</subject><subject>Disease Models, Animal</subject><subject>DNA Gyrase - genetics</subject><subject>DNA Topoisomerase IV - genetics</subject><subject>Drug Resistance, Bacterial</subject><subject>E coli</subject><subject>Enteroaggregative Escherichia coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - physiology</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>extended-spectrum beta lactamases</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Genes</subject><subject>Genotype</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Lepidoptera</subject><subject>Male</subject><subject>Pakistan - epidemiology</subject><subject>Phenotype</subject><subject>phylogroups</subject><subject>Polymorphism, Single Nucleotide</subject><subject>single nucleotide polymorphism</subject><subject>Survival Analysis</subject><subject>Virulence</subject><subject>Virulence Factors - analysis</subject><subject>Virulence Factors - genetics</subject><subject>virulence potential</subject><issn>0903-4641</issn><issn>1600-0463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAURiMEotPCghdAltjQRVr_O1lWVSlIQ5lKBZbWjePMuE3i1E6AYcGz4yHtLJCQ8Ma69rnnyv6y7BXBJyStUxi6E0KFUk-yBZEY55hL9jRb4BKznEtODrLDGG8xJrSQ6nl2QBUvBBFykf1abWzvx-3gDIK-Rut9ZTYQwIw2uJ8wOt8j3yDbp9rDeh3sOh1-s-gimk1CzMYBMr51yEXfwmgjaoLv0GBrB2O6R4MfpnYWuR6t4M7FEfoX2bMG2mhfPuxH2ed3Fzfn7_Plp8sP52fL3PCSq7wsKbMK24pWNSclr1QlmxqXEhgtixoEcKhoDXVtJClKAFpwZljNmspUSpTsKHs7e4fg7ycbR925aGzbQm_9FDUpqCp2U_4LFZQIUrCEvvkLvfVT6NNDdhQnGHMhE3U8Uyb4GINt9BBcB2GrCda7_HTKT__JL7GvH4xT1dl6Tz4GloDTGfjuWrv9t0mfrT4-KvO5I324_bHvgHCnpWJK6K9Xl_p6eXVz_YURLdhvxX-17w</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Khalil, Uzma</creator><creator>Younus, Mahwish</creator><creator>Asghar, Naeem</creator><creator>Siddiqui, Fariha</creator><creator>Gómez-Duarte, Oscar G.</creator><creator>Wren, Brendan W.</creator><creator>Bokhari, Habib</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201610</creationdate><title>Phenotypic and genotypic characterization of enteroaggregative Escherichia coli isolates from pediatric population in Pakistan</title><author>Khalil, Uzma ; Younus, Mahwish ; Asghar, Naeem ; Siddiqui, Fariha ; Gómez-Duarte, Oscar G. ; Wren, Brendan W. ; Bokhari, Habib</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4947-9923e70eb2bd4194b7b6fd096a3298da5a4ab2daddc6189aa2843c3d3fbcb7593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>beta-Lactamases - genetics</topic><topic>Biological Assay</topic><topic>Child, Preschool</topic><topic>Diarrhea - epidemiology</topic><topic>Diarrhea - microbiology</topic><topic>Disease Models, Animal</topic><topic>DNA Gyrase - genetics</topic><topic>DNA Topoisomerase IV - genetics</topic><topic>Drug Resistance, Bacterial</topic><topic>E coli</topic><topic>Enteroaggregative Escherichia coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli - physiology</topic><topic>Escherichia coli Infections - epidemiology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>extended-spectrum beta lactamases</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Genes</topic><topic>Genotype</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Lepidoptera</topic><topic>Male</topic><topic>Pakistan - epidemiology</topic><topic>Phenotype</topic><topic>phylogroups</topic><topic>Polymorphism, Single Nucleotide</topic><topic>single nucleotide polymorphism</topic><topic>Survival Analysis</topic><topic>Virulence</topic><topic>Virulence Factors - analysis</topic><topic>Virulence Factors - genetics</topic><topic>virulence potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khalil, Uzma</creatorcontrib><creatorcontrib>Younus, Mahwish</creatorcontrib><creatorcontrib>Asghar, Naeem</creatorcontrib><creatorcontrib>Siddiqui, Fariha</creatorcontrib><creatorcontrib>Gómez-Duarte, Oscar G.</creatorcontrib><creatorcontrib>Wren, Brendan W.</creatorcontrib><creatorcontrib>Bokhari, Habib</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khalil, Uzma</au><au>Younus, Mahwish</au><au>Asghar, Naeem</au><au>Siddiqui, Fariha</au><au>Gómez-Duarte, Oscar G.</au><au>Wren, Brendan W.</au><au>Bokhari, Habib</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic and genotypic characterization of enteroaggregative Escherichia coli isolates from pediatric population in Pakistan</atitle><jtitle>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</jtitle><addtitle>APMIS</addtitle><date>2016-10</date><risdate>2016</risdate><volume>124</volume><issue>10</issue><spage>872</spage><epage>880</epage><pages>872-880</pages><issn>0903-4641</issn><eissn>1600-0463</eissn><abstract>Enteroaggregative Escherichia coli (EAEC) are a leading cause of diarrhea among children. The objective of this study was to define the frequency of EAEC among diarrheal children from flood‐affected areas as well as sporadic cases, determine multidrug resistance, and evaluation of virulence using an in vivo model of pathogenesis. Stool samples were collected from 225 diarrheal children from 2010 to 2011 from flood‐affected areas as well as from sporadic cases in Pakistan. Identified EAEC isolates were characterized by phylogrouping, antibiotic resistance patterns including the extended‐spectrum beta lactamase spectrum, single nucleotide polymorphism detection in gyrA and parC, and virulence potential using wax worm, G. mellonella. A total of 35 (12.5%) confirmed EAEC isolates were identified among 225 E. coli isolates. EAEC isolates displayed high resistance to tetracycline, ampicillin, and cefaclor. A total of 34.28% were ESBL positive. Single nucleotide polymorphism detection revealed 37.14% and 68.57% isolates were positive for SNPs in gyrA (A660‐T660) and parC (C330‐T330), respectively. Phylogrouping revealed that B2 phylogroup was more prevalent among all EAEC isolates tested followed by D, A, B1, and non‐typeable (NT). Infection of G. mellonella with EAEC showed that killing infective dose was 100% higher than E. coli DH5 alpha control. EAEC are prevalent among Pakistani children with diarrhea, they are highly resistant to antibiotics, and predominantly fall into B2 phylogroup. Epidemiologic surveillance of EAEC and other E. coli pathotypes is critical to assess not only the role of these pathogens in diarrheal disease but also to determine the extent of multidrug resistance among the population.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>27485156</pmid><doi>10.1111/apm.12577</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics beta-Lactamases - genetics Biological Assay Child, Preschool Diarrhea - epidemiology Diarrhea - microbiology Disease Models, Animal DNA Gyrase - genetics DNA Topoisomerase IV - genetics Drug Resistance, Bacterial E coli Enteroaggregative Escherichia coli Escherichia coli Escherichia coli - classification Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli - physiology Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology extended-spectrum beta lactamases Feces - microbiology Female Genes Genotype Humans Infant Infant, Newborn Lepidoptera Male Pakistan - epidemiology Phenotype phylogroups Polymorphism, Single Nucleotide single nucleotide polymorphism Survival Analysis Virulence Virulence Factors - analysis Virulence Factors - genetics virulence potential |
| title | Phenotypic and genotypic characterization of enteroaggregative Escherichia coli isolates from pediatric population in Pakistan |
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