Polymorphism and Expression Profile of Cholecystokinin Type A Receptor in Relation to Gallstone Disease Susceptibility
In the present study, we investigated expression pattern of Cholecystokinin type A receptor (CCKAR) in relation to its commonly studied polymorphism (rs1800857, T/C) in gallstone disease (GSD) patients and controls. A total of 502 subjects (272 GSD and 230 controls) were enrolled, and genotyping was...
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| Veröffentlicht in: | Biochemical genetics 2016-10, Vol.54 (5), p.665-675 |
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| creator | Kazmi, Hasan Raza Chandra, Abhijit Nigam, Jaya Baghel, Kavita Srivastava, Meenu Maurya, Shailendra S. Parmar, Devendra |
| description | In the present study, we investigated expression pattern of Cholecystokinin type A receptor (CCKAR) in relation to its commonly studied polymorphism (rs1800857, T/C) in gallstone disease (GSD) patients and controls. A total of 502 subjects (272 GSD and 230 controls) were enrolled, and genotyping was performed by evaluating restriction fragments of
PstI
digested DNA. For analyzing expression pattern of CCKAR in relation to polymorphism, gallbladder tissue samples from 80 subjects (GSD—55; control—25) were studied. Expression of CCKAR mRNA was evaluated by reverse transcriptase-PCR and confirmed using real-time PCR. Protein expression was evaluated by enzyme-linked immunosorbent assay. We observed significantly (
p
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| doi_str_mv | 10.1007/s10528-016-9745-x |
| format | Article |
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PstI
digested DNA. For analyzing expression pattern of CCKAR in relation to polymorphism, gallbladder tissue samples from 80 subjects (GSD—55; control—25) were studied. Expression of CCKAR mRNA was evaluated by reverse transcriptase-PCR and confirmed using real-time PCR. Protein expression was evaluated by enzyme-linked immunosorbent assay. We observed significantly (
p
< 0.0001) lower expression of CCKAR mRNA and protein in GSD tissues as compared with control. Significantly higher frequency of A1/A1 genotype (C/T transition) (
p
= 0.0005) was observed for GSD as compared with control. Expression of CCKAR protein was found to be significantly lower (
p
< 0.0001) in A1/A1 genotype as compared with other genotypes for GSD patients. Perhaps, this is the first report providing evidence of alteration in CCKAR expression in relation to its polymorphism elucidating the molecular pathway of the disease. Additional investigations with lager sample size are needed to confirm these findings.</description><identifier>ISSN: 0006-2928</identifier><identifier>EISSN: 1573-4927</identifier><identifier>DOI: 10.1007/s10528-016-9745-x</identifier><identifier>PMID: 27287528</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adult ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Disease Susceptibility ; Down-Regulation ; Female ; Gallstones - genetics ; Gallstones - metabolism ; Genotypes ; Genotyping Techniques ; Human Genetics ; Humans ; India ; Male ; Medical Microbiology ; Middle Aged ; Original Article ; Polymorphism, Single Nucleotide ; Receptor, Cholecystokinin A - genetics ; Receptor, Cholecystokinin A - metabolism ; Tissue Distribution ; Young Adult ; Zoology</subject><ispartof>Biochemical genetics, 2016-10, Vol.54 (5), p.665-675</ispartof><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-e5ba3ad5772562ea19b5440fdd81fa148edec7b3963da13f6cf016c23d5d37af3</citedby><cites>FETCH-LOGICAL-c405t-e5ba3ad5772562ea19b5440fdd81fa148edec7b3963da13f6cf016c23d5d37af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10528-016-9745-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10528-016-9745-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27287528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kazmi, Hasan Raza</creatorcontrib><creatorcontrib>Chandra, Abhijit</creatorcontrib><creatorcontrib>Nigam, Jaya</creatorcontrib><creatorcontrib>Baghel, Kavita</creatorcontrib><creatorcontrib>Srivastava, Meenu</creatorcontrib><creatorcontrib>Maurya, Shailendra S.</creatorcontrib><creatorcontrib>Parmar, Devendra</creatorcontrib><title>Polymorphism and Expression Profile of Cholecystokinin Type A Receptor in Relation to Gallstone Disease Susceptibility</title><title>Biochemical genetics</title><addtitle>Biochem Genet</addtitle><addtitle>Biochem Genet</addtitle><description>In the present study, we investigated expression pattern of Cholecystokinin type A receptor (CCKAR) in relation to its commonly studied polymorphism (rs1800857, T/C) in gallstone disease (GSD) patients and controls. A total of 502 subjects (272 GSD and 230 controls) were enrolled, and genotyping was performed by evaluating restriction fragments of
PstI
digested DNA. For analyzing expression pattern of CCKAR in relation to polymorphism, gallbladder tissue samples from 80 subjects (GSD—55; control—25) were studied. Expression of CCKAR mRNA was evaluated by reverse transcriptase-PCR and confirmed using real-time PCR. Protein expression was evaluated by enzyme-linked immunosorbent assay. We observed significantly (
p
< 0.0001) lower expression of CCKAR mRNA and protein in GSD tissues as compared with control. Significantly higher frequency of A1/A1 genotype (C/T transition) (
p
= 0.0005) was observed for GSD as compared with control. Expression of CCKAR protein was found to be significantly lower (
p
< 0.0001) in A1/A1 genotype as compared with other genotypes for GSD patients. Perhaps, this is the first report providing evidence of alteration in CCKAR expression in relation to its polymorphism elucidating the molecular pathway of the disease. Additional investigations with lager sample size are needed to confirm these findings.</description><subject>Adult</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Disease Susceptibility</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Gallstones - genetics</subject><subject>Gallstones - metabolism</subject><subject>Genotypes</subject><subject>Genotyping Techniques</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>India</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Middle Aged</subject><subject>Original Article</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Receptor, Cholecystokinin A - genetics</subject><subject>Receptor, Cholecystokinin A - metabolism</subject><subject>Tissue Distribution</subject><subject>Young Adult</subject><subject>Zoology</subject><issn>0006-2928</issn><issn>1573-4927</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kc1u1DAUhS0EokPLA7BBltiwCfg3dpbVUNpKlVq1ZW058Q11ceJgJ2jy9ng0BSGkriz7fufcIx-E3lHyiRKiPmdKJNMVoXXVKCGr3Qu0oVLxSjRMvUQbQkhdsYbpI_Qm58dybYgQr9ERU0yrIt2gXzcxrENM04PPA7ajw2e7KUHOPo74JsXeB8Cxx9uHGKBb8xx_-NGP-H6dAJ_iW-hgmmPC5ekWgp33sjnicxtCYUfAX3wGmwHfLXmP-tYHP68n6FVvQ4a3T-cx-vb17H57UV1dn19uT6-qThA5VyBby62TSjFZM7C0aaUQpHdO095SocFBp1re1NxZyvu668tndIw76biyPT9GHw--U4o_F8izGXzJEYIdIS7ZUM2U1lwrUtAP_6GPcUljSVcoqokozqJQ9EB1KeacoDdT8oNNq6HE7Esxh1JMoc2-FLMrmvdPzks7gPur-NNCAdgByGU0fof0z-pnXX8DK2qZ2g</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Kazmi, Hasan Raza</creator><creator>Chandra, Abhijit</creator><creator>Nigam, Jaya</creator><creator>Baghel, Kavita</creator><creator>Srivastava, Meenu</creator><creator>Maurya, Shailendra S.</creator><creator>Parmar, Devendra</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20161001</creationdate><title>Polymorphism and Expression Profile of Cholecystokinin Type A Receptor in Relation to Gallstone Disease Susceptibility</title><author>Kazmi, Hasan Raza ; Chandra, Abhijit ; Nigam, Jaya ; Baghel, Kavita ; Srivastava, Meenu ; Maurya, Shailendra S. ; Parmar, Devendra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-e5ba3ad5772562ea19b5440fdd81fa148edec7b3963da13f6cf016c23d5d37af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Disease Susceptibility</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Gallstones - genetics</topic><topic>Gallstones - metabolism</topic><topic>Genotypes</topic><topic>Genotyping Techniques</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>India</topic><topic>Male</topic><topic>Medical Microbiology</topic><topic>Middle Aged</topic><topic>Original Article</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Receptor, Cholecystokinin A - genetics</topic><topic>Receptor, Cholecystokinin A - metabolism</topic><topic>Tissue Distribution</topic><topic>Young Adult</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kazmi, Hasan Raza</creatorcontrib><creatorcontrib>Chandra, Abhijit</creatorcontrib><creatorcontrib>Nigam, Jaya</creatorcontrib><creatorcontrib>Baghel, Kavita</creatorcontrib><creatorcontrib>Srivastava, Meenu</creatorcontrib><creatorcontrib>Maurya, Shailendra S.</creatorcontrib><creatorcontrib>Parmar, Devendra</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Biochemical genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kazmi, Hasan Raza</au><au>Chandra, Abhijit</au><au>Nigam, Jaya</au><au>Baghel, Kavita</au><au>Srivastava, Meenu</au><au>Maurya, Shailendra S.</au><au>Parmar, Devendra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymorphism and Expression Profile of Cholecystokinin Type A Receptor in Relation to Gallstone Disease Susceptibility</atitle><jtitle>Biochemical genetics</jtitle><stitle>Biochem Genet</stitle><addtitle>Biochem Genet</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>54</volume><issue>5</issue><spage>665</spage><epage>675</epage><pages>665-675</pages><issn>0006-2928</issn><eissn>1573-4927</eissn><abstract>In the present study, we investigated expression pattern of Cholecystokinin type A receptor (CCKAR) in relation to its commonly studied polymorphism (rs1800857, T/C) in gallstone disease (GSD) patients and controls. A total of 502 subjects (272 GSD and 230 controls) were enrolled, and genotyping was performed by evaluating restriction fragments of
PstI
digested DNA. For analyzing expression pattern of CCKAR in relation to polymorphism, gallbladder tissue samples from 80 subjects (GSD—55; control—25) were studied. Expression of CCKAR mRNA was evaluated by reverse transcriptase-PCR and confirmed using real-time PCR. Protein expression was evaluated by enzyme-linked immunosorbent assay. We observed significantly (
p
< 0.0001) lower expression of CCKAR mRNA and protein in GSD tissues as compared with control. Significantly higher frequency of A1/A1 genotype (C/T transition) (
p
= 0.0005) was observed for GSD as compared with control. Expression of CCKAR protein was found to be significantly lower (
p
< 0.0001) in A1/A1 genotype as compared with other genotypes for GSD patients. Perhaps, this is the first report providing evidence of alteration in CCKAR expression in relation to its polymorphism elucidating the molecular pathway of the disease. Additional investigations with lager sample size are needed to confirm these findings.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>27287528</pmid><doi>10.1007/s10528-016-9745-x</doi><tpages>11</tpages></addata></record> |
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| ispartof | Biochemical genetics, 2016-10, Vol.54 (5), p.665-675 |
| issn | 0006-2928 1573-4927 |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Adult Biochemistry Biomedical and Life Sciences Biomedicine Disease Susceptibility Down-Regulation Female Gallstones - genetics Gallstones - metabolism Genotypes Genotyping Techniques Human Genetics Humans India Male Medical Microbiology Middle Aged Original Article Polymorphism, Single Nucleotide Receptor, Cholecystokinin A - genetics Receptor, Cholecystokinin A - metabolism Tissue Distribution Young Adult Zoology |
| title | Polymorphism and Expression Profile of Cholecystokinin Type A Receptor in Relation to Gallstone Disease Susceptibility |
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