Expression Profiling of Developing Zebrafish Retinal Cells
During retinal development, a variety of different types of neurons are produced. Understanding how each of these types of retinal nerve cells is generated is important from a developmental biology perspective. It is equally important if one is interested in how to regenerate cells after an injury o...
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| Veröffentlicht in: | Zebrafish 2016-08, Vol.13 (4), p.272-280 |
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| creator | Mullally, Madelyn Albrecht, Caitlin Horton, Mary Laboissonniere, Lauren A. Goetz, Jillian J. Chowdhury, Rebecca Manning, Alicia Wester, Andrea K. Bose, Quinton Trimarchi, Jeffrey M. |
| description | During retinal development, a variety of different types of neurons are produced. Understanding how each of these types of retinal nerve cells is generated is important from a developmental biology perspective. It is equally important if one is interested in how to regenerate cells after an injury or a disease. To gain more insight into how retinal neurons develop in the zebrafish, we performed single-cell mRNA profiling and
in situ
hybridizations (ISHs) on retinal sections and whole-mount zebrafish. Through the series of ISHs, designed and performed solely by undergraduate students in the laboratory, we were able to retrospectively identify our single-cell mRNA profiles as most likely coming from developing amacrine cells. Further analysis of these profiles will reveal genes that can be mutated using genome editing techniques. Together these studies increase our knowledge of the genes driving development of different cell types in the zebrafish retina. |
| doi_str_mv | 10.1089/zeb.2015.1184 |
| format | Article |
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in situ
hybridizations (ISHs) on retinal sections and whole-mount zebrafish. Through the series of ISHs, designed and performed solely by undergraduate students in the laboratory, we were able to retrospectively identify our single-cell mRNA profiles as most likely coming from developing amacrine cells. Further analysis of these profiles will reveal genes that can be mutated using genome editing techniques. Together these studies increase our knowledge of the genes driving development of different cell types in the zebrafish retina.</description><identifier>ISSN: 1545-8547</identifier><identifier>EISSN: 1557-8542</identifier><identifier>DOI: 10.1089/zeb.2015.1184</identifier><identifier>PMID: 26982811</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Amacrine cells ; Amacrine Cells - cytology ; Amacrine Cells - metabolism ; Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - growth & development ; Animals, Genetically Modified - metabolism ; Biology ; Danio rerio ; Developmental biology ; EduFish ; Freshwater ; Freshwater fishes ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Genes ; Genomes ; mRNA ; Nerves ; Neurons ; Profiles ; Profiling ; Retina ; Retina - growth & development ; Retina - metabolism ; Retinal cells ; Retinal Ganglion Cells - cytology ; Retinal Ganglion Cells - metabolism ; Special Section: Zebrafish in Education ; Zebrafish ; Zebrafish - genetics ; Zebrafish - growth & development ; Zebrafish - metabolism</subject><ispartof>Zebrafish, 2016-08, Vol.13 (4), p.272-280</ispartof><rights>2016, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2016, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-98a84fc3c6882e6bbf52071ccf2242fcfd224e0ef6bd3d0145e3a7d1cd738d353</citedby><cites>FETCH-LOGICAL-c398t-98a84fc3c6882e6bbf52071ccf2242fcfd224e0ef6bd3d0145e3a7d1cd738d353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27906,27907</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26982811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mullally, Madelyn</creatorcontrib><creatorcontrib>Albrecht, Caitlin</creatorcontrib><creatorcontrib>Horton, Mary</creatorcontrib><creatorcontrib>Laboissonniere, Lauren A.</creatorcontrib><creatorcontrib>Goetz, Jillian J.</creatorcontrib><creatorcontrib>Chowdhury, Rebecca</creatorcontrib><creatorcontrib>Manning, Alicia</creatorcontrib><creatorcontrib>Wester, Andrea K.</creatorcontrib><creatorcontrib>Bose, Quinton</creatorcontrib><creatorcontrib>Trimarchi, Jeffrey M.</creatorcontrib><title>Expression Profiling of Developing Zebrafish Retinal Cells</title><title>Zebrafish</title><addtitle>Zebrafish</addtitle><description>During retinal development, a variety of different types of neurons are produced. Understanding how each of these types of retinal nerve cells is generated is important from a developmental biology perspective. It is equally important if one is interested in how to regenerate cells after an injury or a disease. To gain more insight into how retinal neurons develop in the zebrafish, we performed single-cell mRNA profiling and
in situ
hybridizations (ISHs) on retinal sections and whole-mount zebrafish. Through the series of ISHs, designed and performed solely by undergraduate students in the laboratory, we were able to retrospectively identify our single-cell mRNA profiles as most likely coming from developing amacrine cells. Further analysis of these profiles will reveal genes that can be mutated using genome editing techniques. Together these studies increase our knowledge of the genes driving development of different cell types in the zebrafish retina.</description><subject>Amacrine cells</subject><subject>Amacrine Cells - cytology</subject><subject>Amacrine Cells - metabolism</subject><subject>Animals</subject><subject>Animals, Genetically Modified - genetics</subject><subject>Animals, Genetically Modified - growth & development</subject><subject>Animals, Genetically Modified - metabolism</subject><subject>Biology</subject><subject>Danio rerio</subject><subject>Developmental biology</subject><subject>EduFish</subject><subject>Freshwater</subject><subject>Freshwater fishes</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>Genomes</subject><subject>mRNA</subject><subject>Nerves</subject><subject>Neurons</subject><subject>Profiles</subject><subject>Profiling</subject><subject>Retina</subject><subject>Retina - growth & development</subject><subject>Retina - metabolism</subject><subject>Retinal cells</subject><subject>Retinal Ganglion Cells - cytology</subject><subject>Retinal Ganglion Cells - metabolism</subject><subject>Special Section: Zebrafish in Education</subject><subject>Zebrafish</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish - growth & development</subject><subject>Zebrafish - metabolism</subject><issn>1545-8547</issn><issn>1557-8542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAURi0EoqUwsqJILCwpfiYOGyrlIVUCIVhYLMe5hlRpEuwEAb-eWC0MLJ3uQ0ef7j0IHRM8JVhm59-QTykmYkqI5DtoTIRIYyk43Q09F6FPR-jA-yXGjGWM76MRTTJJJSFjdDH_bB14XzZ19OAaW1Zl_Ro1NrqCD6iaNkwvkDttS_8WPUJX1rqKZlBV_hDtWV15ONrUCXq-nj_NbuPF_c3d7HIRG5bJLs6kltwaZhIpKSR5bgXFKTHGUsqpNbYYKmCwSV6wAhMugOm0IKZImSyYYBN0ts5tXfPeg-_UqvRmuEDX0PReEUlTKRlP8HY0zTIhBgch9fQfumx6NzznFSWY4YRJwgcqXlPGNd47sKp15Uq7L0WwCv7V4F8F_yr4H_iTTWqfr6D4o3-FDwBbA2Gt67oqIQfXbYn9ASsUkFo</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Mullally, Madelyn</creator><creator>Albrecht, Caitlin</creator><creator>Horton, Mary</creator><creator>Laboissonniere, Lauren A.</creator><creator>Goetz, Jillian J.</creator><creator>Chowdhury, Rebecca</creator><creator>Manning, Alicia</creator><creator>Wester, Andrea K.</creator><creator>Bose, Quinton</creator><creator>Trimarchi, Jeffrey M.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160801</creationdate><title>Expression Profiling of Developing Zebrafish Retinal Cells</title><author>Mullally, Madelyn ; 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in situ
hybridizations (ISHs) on retinal sections and whole-mount zebrafish. Through the series of ISHs, designed and performed solely by undergraduate students in the laboratory, we were able to retrospectively identify our single-cell mRNA profiles as most likely coming from developing amacrine cells. Further analysis of these profiles will reveal genes that can be mutated using genome editing techniques. Together these studies increase our knowledge of the genes driving development of different cell types in the zebrafish retina.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>26982811</pmid><doi>10.1089/zeb.2015.1184</doi><tpages>9</tpages></addata></record> |
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| subjects | Amacrine cells Amacrine Cells - cytology Amacrine Cells - metabolism Animals Animals, Genetically Modified - genetics Animals, Genetically Modified - growth & development Animals, Genetically Modified - metabolism Biology Danio rerio Developmental biology EduFish Freshwater Freshwater fishes Gene Expression Profiling Gene Expression Regulation, Developmental Genes Genomes mRNA Nerves Neurons Profiles Profiling Retina Retina - growth & development Retina - metabolism Retinal cells Retinal Ganglion Cells - cytology Retinal Ganglion Cells - metabolism Special Section: Zebrafish in Education Zebrafish Zebrafish - genetics Zebrafish - growth & development Zebrafish - metabolism |
| title | Expression Profiling of Developing Zebrafish Retinal Cells |
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