'Forrest' resistance to the soybean cyst nematode is bigenic : saturation mapping of the Rhg1 and Rhg4 loci
Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv 'Forrest' is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (...
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| Veröffentlicht in: | Theoretical and applied genetics 2001-10, Vol.103 (5), p.710-717 |
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| creator | MEKSEM, K PANTAZOPOULOS, P NJITI, V. N HYTEN, L. D ARELLI, P. R LIGHTFOOT, D. A |
| description | Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv 'Forrest' is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of ForrestEssex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg1 and Rhg4. The two QTLs involved in resistance to SCN were strongly associated with the AFLP marker EATGMCGA87 (P=0.0001, R super(2)=24.5%) on linkage group G, and the AFLP marker ECCGMAAC405 (P=0.0001, R super(2) =26.2%) on linkage group A2. Two- way analysis of variance showed epistasic interaction (P=0.0001, R super(2) =16%) between the two loci controlling SCN resistance in EssexForrest recombinant inbred lines. Considering the two loci as qualitative genes and the resistance as female index FI 5%, jointly the two loci explained over 98% of the resistance. The locations of the two QTLs were confirmed in the NILs populations. Therefore SCN resistance in ForrestEssex is bigenic. High-efficiency marker-assisted selection can be performed using the markers to develop cultivars with stable resistance to SCN. |
| doi_str_mv | 10.1007/s001220100597 |
| format | Article |
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N ; HYTEN, L. D ; ARELLI, P. R ; LIGHTFOOT, D. A</creator><creatorcontrib>MEKSEM, K ; PANTAZOPOULOS, P ; NJITI, V. N ; HYTEN, L. D ; ARELLI, P. R ; LIGHTFOOT, D. A</creatorcontrib><description>Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv 'Forrest' is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of ForrestEssex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg1 and Rhg4. The two QTLs involved in resistance to SCN were strongly associated with the AFLP marker EATGMCGA87 (P=0.0001, R super(2)=24.5%) on linkage group G, and the AFLP marker ECCGMAAC405 (P=0.0001, R super(2) =26.2%) on linkage group A2. Two- way analysis of variance showed epistasic interaction (P=0.0001, R super(2) =16%) between the two loci controlling SCN resistance in EssexForrest recombinant inbred lines. Considering the two loci as qualitative genes and the resistance as female index FI 5%, jointly the two loci explained over 98% of the resistance. The locations of the two QTLs were confirmed in the NILs populations. Therefore SCN resistance in ForrestEssex is bigenic. High-efficiency marker-assisted selection can be performed using the markers to develop cultivars with stable resistance to SCN.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220100597</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; Classical genetics, quantitative genetics, hybrids ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. 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R</creatorcontrib><creatorcontrib>LIGHTFOOT, D. A</creatorcontrib><title>'Forrest' resistance to the soybean cyst nematode is bigenic : saturation mapping of the Rhg1 and Rhg4 loci</title><title>Theoretical and applied genetics</title><description>Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv 'Forrest' is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of ForrestEssex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg1 and Rhg4. The two QTLs involved in resistance to SCN were strongly associated with the AFLP marker EATGMCGA87 (P=0.0001, R super(2)=24.5%) on linkage group G, and the AFLP marker ECCGMAAC405 (P=0.0001, R super(2) =26.2%) on linkage group A2. Two- way analysis of variance showed epistasic interaction (P=0.0001, R super(2) =16%) between the two loci controlling SCN resistance in EssexForrest recombinant inbred lines. Considering the two loci as qualitative genes and the resistance as female index FI 5%, jointly the two loci explained over 98% of the resistance. The locations of the two QTLs were confirmed in the NILs populations. Therefore SCN resistance in ForrestEssex is bigenic. High-efficiency marker-assisted selection can be performed using the markers to develop cultivars with stable resistance to SCN.</description><subject>Biological and medical sciences</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Glycine max</subject><subject>Heterodera glycines</subject><subject>Pteridophyta, spermatophyta</subject><subject>Rhg1 gene</subject><subject>Rhg4 gene</subject><subject>Vegetals</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNotT7FOwzAUtBBIlMLI7gU6BZ5f7DhhQ4gCUiUkBHPkOk5rSOxgu0P_HgNd7m64O90RcsnghgHI2wjAECFr0cgjMmO8xAKR4zGZAXAohBR4Ss5i_AQAFFDOyNdi6UMwMS1oRhuTctrQ5GnaGhr9fm2Uo3ofE3VmVMl3htpI13ZjnNX0jkaVdkEl6x0d1TRZt6G-_wu_bTeMKtf9Ck4Hr-05OenVEM3FgefkY_n4_vBcrF6fXh7uV8WEjKfCdLJEUUmohKkbgLVGLAEro0CLHjlrRB5fS91J0Xe1xK7jja4NMAXQN3U5J9f_vVPw37v8rR1t1GYYlDN-F1tWo-S8KrPx6mBUUauhD_m8je0U7KjCvmUcZJ37yh82kGZv</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>MEKSEM, K</creator><creator>PANTAZOPOULOS, P</creator><creator>NJITI, V. 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Biological and molecular evolution</topic><topic>Glycine max</topic><topic>Heterodera glycines</topic><topic>Pteridophyta, spermatophyta</topic><topic>Rhg1 gene</topic><topic>Rhg4 gene</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MEKSEM, K</creatorcontrib><creatorcontrib>PANTAZOPOULOS, P</creatorcontrib><creatorcontrib>NJITI, V. N</creatorcontrib><creatorcontrib>HYTEN, L. D</creatorcontrib><creatorcontrib>ARELLI, P. R</creatorcontrib><creatorcontrib>LIGHTFOOT, D. A</creatorcontrib><collection>Pascal-Francis</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MEKSEM, K</au><au>PANTAZOPOULOS, P</au><au>NJITI, V. N</au><au>HYTEN, L. D</au><au>ARELLI, P. R</au><au>LIGHTFOOT, D. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>'Forrest' resistance to the soybean cyst nematode is bigenic : saturation mapping of the Rhg1 and Rhg4 loci</atitle><jtitle>Theoretical and applied genetics</jtitle><date>2001-10-01</date><risdate>2001</risdate><volume>103</volume><issue>5</issue><spage>710</spage><epage>717</epage><pages>710-717</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv 'Forrest' is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of ForrestEssex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg1 and Rhg4. The two QTLs involved in resistance to SCN were strongly associated with the AFLP marker EATGMCGA87 (P=0.0001, R super(2)=24.5%) on linkage group G, and the AFLP marker ECCGMAAC405 (P=0.0001, R super(2) =26.2%) on linkage group A2. Two- way analysis of variance showed epistasic interaction (P=0.0001, R super(2) =16%) between the two loci controlling SCN resistance in EssexForrest recombinant inbred lines. Considering the two loci as qualitative genes and the resistance as female index FI 5%, jointly the two loci explained over 98% of the resistance. The locations of the two QTLs were confirmed in the NILs populations. Therefore SCN resistance in ForrestEssex is bigenic. High-efficiency marker-assisted selection can be performed using the markers to develop cultivars with stable resistance to SCN.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220100597</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Glycine max Heterodera glycines Pteridophyta, spermatophyta Rhg1 gene Rhg4 gene Vegetals |
| title | 'Forrest' resistance to the soybean cyst nematode is bigenic : saturation mapping of the Rhg1 and Rhg4 loci |
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