Novel technique for high-precision stereotactic irradiation of mouse brains
Background and purpose Small animal irradiation systems were developed for preclinical evaluation of tumor therapy closely resembling the clinical situation. Mostly only clinical LINACs are available, so protocols for small animal partial body irradiation using a conventional clinical system are ess...
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| Veröffentlicht in: | Strahlentherapie und Onkologie 2016-11, Vol.192 (11), p.806-814 |
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| creator | Hartmann, J. Wölfelschneider, J. Stache, C. Buslei, R. Derer, A. Schwarz, M. Bäuerle, T. Fietkau, R. Gaipl, U. S. Bert, C. Hölsken, A. Frey, B. |
| description | Background and purpose
Small animal irradiation systems were developed for preclinical evaluation of tumor therapy closely resembling the clinical situation. Mostly only clinical LINACs are available, so protocols for small animal partial body irradiation using a conventional clinical system are essential. This study defines a protocol for conformal brain tumor irradiations in mice.
Materials and methods
CT and MRI images were used to demarcate the target volume and organs at risk. Three 6 MV photon beams were planned for a total dose of 10 fractions of 1.8 Gy. The mouse position in a dedicated applicator was verified by an X‑ray patient positioning system before each irradiation. Dosimetric verifications (using ionization chambers and films) were performed. Irradiation-induced DNA damage was analyzed to verify the treatment effects on the cellular level.
Results
The defined treatment protocol and the applied fractionation scheme were feasible. The in-house developed applicator was suitable for individual positioning at submillimeter accuracy of anesthetized mice during irradiation, altogether performed in less than 10 min. All mice tolerated the treatment well. Measured dose values perfectly matched the nominal values from treatment planning. Cellular response was restricted to the target volume.
Conclusion
Clinical LINAC-based irradiations of mice offer the potential to treat orthotopic tumors conformably. Especially with respect to lateral penumbra, dedicated small animal irradiation systems exceed the clinical LINAC solution. |
| doi_str_mv | 10.1007/s00066-016-1014-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1826717603</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1826717603</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-834e32b7412edf6aa9f9b00224faae9209ade8dc174467749312cab4f1c65213</originalsourceid><addsrcrecordid>eNp1kMFqGzEQhkVpqB23D5BLWOilFyUzkrxaHYNpm5DQXHLITWi1s7aMvXKldSBvXxk7JRR6msN888_Px9gFwhUC6OsMAHXNAWuOgIo3H9gUlTQcjHn-yKaA2nCN82bCznNeQwGVUZ_YRGgFQjZmyu5_xRfaVCP51RB-76nqY6pWYbniu0Q-5BCHKo-UKI7Oj8FXISXXBTceFrGvtnGfqWqTC0P-zM56t8n05TRn7OnH96fFLX94_Hm3uHngXmox8kYqkqLVCgV1fe2c6U0LIITqnSMjwLiOms6jVqrWWhmJwrtW9ejruUA5Y9-OsbsUS-M82m3InjYbN1BpY7ERtUZdgyzo13_QddynoZQrlCxZjSrYjOGR8inmnKi3uxS2Lr1aBHsQbY-ibfFnD6JtU24uT8n7dkvd34s3swUQRyCX1bCk9O71f1P_AOjZh_s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1832138460</pqid></control><display><type>article</type><title>Novel technique for high-precision stereotactic irradiation of mouse brains</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Hartmann, J. ; Wölfelschneider, J. ; Stache, C. ; Buslei, R. ; Derer, A. ; Schwarz, M. ; Bäuerle, T. ; Fietkau, R. ; Gaipl, U. S. ; Bert, C. ; Hölsken, A. ; Frey, B.</creator><creatorcontrib>Hartmann, J. ; Wölfelschneider, J. ; Stache, C. ; Buslei, R. ; Derer, A. ; Schwarz, M. ; Bäuerle, T. ; Fietkau, R. ; Gaipl, U. S. ; Bert, C. ; Hölsken, A. ; Frey, B.</creatorcontrib><description>Background and purpose
Small animal irradiation systems were developed for preclinical evaluation of tumor therapy closely resembling the clinical situation. Mostly only clinical LINACs are available, so protocols for small animal partial body irradiation using a conventional clinical system are essential. This study defines a protocol for conformal brain tumor irradiations in mice.
Materials and methods
CT and MRI images were used to demarcate the target volume and organs at risk. Three 6 MV photon beams were planned for a total dose of 10 fractions of 1.8 Gy. The mouse position in a dedicated applicator was verified by an X‑ray patient positioning system before each irradiation. Dosimetric verifications (using ionization chambers and films) were performed. Irradiation-induced DNA damage was analyzed to verify the treatment effects on the cellular level.
Results
The defined treatment protocol and the applied fractionation scheme were feasible. The in-house developed applicator was suitable for individual positioning at submillimeter accuracy of anesthetized mice during irradiation, altogether performed in less than 10 min. All mice tolerated the treatment well. Measured dose values perfectly matched the nominal values from treatment planning. Cellular response was restricted to the target volume.
Conclusion
Clinical LINAC-based irradiations of mice offer the potential to treat orthotopic tumors conformably. Especially with respect to lateral penumbra, dedicated small animal irradiation systems exceed the clinical LINAC solution.</description><identifier>ISSN: 0179-7158</identifier><identifier>EISSN: 1439-099X</identifier><identifier>DOI: 10.1007/s00066-016-1014-8</identifier><identifier>PMID: 27402389</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Brain Neoplasms - diagnostic imaging ; Brain Neoplasms - radiotherapy ; Brain Neoplasms - veterinary ; Cell Line, Tumor ; Medicine ; Medicine & Public Health ; Mice ; Oncology ; Original Article ; Patient Positioning - veterinary ; Radiosurgery - veterinary ; Radiotherapy ; Radiotherapy Dosage ; Radiotherapy Planning, Computer-Assisted - veterinary ; Radiotherapy, Image-Guided - veterinary ; Reproducibility of Results ; Sensitivity and Specificity ; Treatment Outcome</subject><ispartof>Strahlentherapie und Onkologie, 2016-11, Vol.192 (11), p.806-814</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-834e32b7412edf6aa9f9b00224faae9209ade8dc174467749312cab4f1c65213</citedby><cites>FETCH-LOGICAL-c372t-834e32b7412edf6aa9f9b00224faae9209ade8dc174467749312cab4f1c65213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00066-016-1014-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00066-016-1014-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27402389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hartmann, J.</creatorcontrib><creatorcontrib>Wölfelschneider, J.</creatorcontrib><creatorcontrib>Stache, C.</creatorcontrib><creatorcontrib>Buslei, R.</creatorcontrib><creatorcontrib>Derer, A.</creatorcontrib><creatorcontrib>Schwarz, M.</creatorcontrib><creatorcontrib>Bäuerle, T.</creatorcontrib><creatorcontrib>Fietkau, R.</creatorcontrib><creatorcontrib>Gaipl, U. S.</creatorcontrib><creatorcontrib>Bert, C.</creatorcontrib><creatorcontrib>Hölsken, A.</creatorcontrib><creatorcontrib>Frey, B.</creatorcontrib><title>Novel technique for high-precision stereotactic irradiation of mouse brains</title><title>Strahlentherapie und Onkologie</title><addtitle>Strahlenther Onkol</addtitle><addtitle>Strahlenther Onkol</addtitle><description>Background and purpose
Small animal irradiation systems were developed for preclinical evaluation of tumor therapy closely resembling the clinical situation. Mostly only clinical LINACs are available, so protocols for small animal partial body irradiation using a conventional clinical system are essential. This study defines a protocol for conformal brain tumor irradiations in mice.
Materials and methods
CT and MRI images were used to demarcate the target volume and organs at risk. Three 6 MV photon beams were planned for a total dose of 10 fractions of 1.8 Gy. The mouse position in a dedicated applicator was verified by an X‑ray patient positioning system before each irradiation. Dosimetric verifications (using ionization chambers and films) were performed. Irradiation-induced DNA damage was analyzed to verify the treatment effects on the cellular level.
Results
The defined treatment protocol and the applied fractionation scheme were feasible. The in-house developed applicator was suitable for individual positioning at submillimeter accuracy of anesthetized mice during irradiation, altogether performed in less than 10 min. All mice tolerated the treatment well. Measured dose values perfectly matched the nominal values from treatment planning. Cellular response was restricted to the target volume.
Conclusion
Clinical LINAC-based irradiations of mice offer the potential to treat orthotopic tumors conformably. Especially with respect to lateral penumbra, dedicated small animal irradiation systems exceed the clinical LINAC solution.</description><subject>Animals</subject><subject>Brain Neoplasms - diagnostic imaging</subject><subject>Brain Neoplasms - radiotherapy</subject><subject>Brain Neoplasms - veterinary</subject><subject>Cell Line, Tumor</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Patient Positioning - veterinary</subject><subject>Radiosurgery - veterinary</subject><subject>Radiotherapy</subject><subject>Radiotherapy Dosage</subject><subject>Radiotherapy Planning, Computer-Assisted - veterinary</subject><subject>Radiotherapy, Image-Guided - veterinary</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Treatment Outcome</subject><issn>0179-7158</issn><issn>1439-099X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kMFqGzEQhkVpqB23D5BLWOilFyUzkrxaHYNpm5DQXHLITWi1s7aMvXKldSBvXxk7JRR6msN888_Px9gFwhUC6OsMAHXNAWuOgIo3H9gUlTQcjHn-yKaA2nCN82bCznNeQwGVUZ_YRGgFQjZmyu5_xRfaVCP51RB-76nqY6pWYbniu0Q-5BCHKo-UKI7Oj8FXISXXBTceFrGvtnGfqWqTC0P-zM56t8n05TRn7OnH96fFLX94_Hm3uHngXmox8kYqkqLVCgV1fe2c6U0LIITqnSMjwLiOms6jVqrWWhmJwrtW9ejruUA5Y9-OsbsUS-M82m3InjYbN1BpY7ERtUZdgyzo13_QddynoZQrlCxZjSrYjOGR8inmnKi3uxS2Lr1aBHsQbY-ibfFnD6JtU24uT8n7dkvd34s3swUQRyCX1bCk9O71f1P_AOjZh_s</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Hartmann, J.</creator><creator>Wölfelschneider, J.</creator><creator>Stache, C.</creator><creator>Buslei, R.</creator><creator>Derer, A.</creator><creator>Schwarz, M.</creator><creator>Bäuerle, T.</creator><creator>Fietkau, R.</creator><creator>Gaipl, U. S.</creator><creator>Bert, C.</creator><creator>Hölsken, A.</creator><creator>Frey, B.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20161101</creationdate><title>Novel technique for high-precision stereotactic irradiation of mouse brains</title><author>Hartmann, J. ; Wölfelschneider, J. ; Stache, C. ; Buslei, R. ; Derer, A. ; Schwarz, M. ; Bäuerle, T. ; Fietkau, R. ; Gaipl, U. S. ; Bert, C. ; Hölsken, A. ; Frey, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-834e32b7412edf6aa9f9b00224faae9209ade8dc174467749312cab4f1c65213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Brain Neoplasms - diagnostic imaging</topic><topic>Brain Neoplasms - radiotherapy</topic><topic>Brain Neoplasms - veterinary</topic><topic>Cell Line, Tumor</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Patient Positioning - veterinary</topic><topic>Radiosurgery - veterinary</topic><topic>Radiotherapy</topic><topic>Radiotherapy Dosage</topic><topic>Radiotherapy Planning, Computer-Assisted - veterinary</topic><topic>Radiotherapy, Image-Guided - veterinary</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hartmann, J.</creatorcontrib><creatorcontrib>Wölfelschneider, J.</creatorcontrib><creatorcontrib>Stache, C.</creatorcontrib><creatorcontrib>Buslei, R.</creatorcontrib><creatorcontrib>Derer, A.</creatorcontrib><creatorcontrib>Schwarz, M.</creatorcontrib><creatorcontrib>Bäuerle, T.</creatorcontrib><creatorcontrib>Fietkau, R.</creatorcontrib><creatorcontrib>Gaipl, U. S.</creatorcontrib><creatorcontrib>Bert, C.</creatorcontrib><creatorcontrib>Hölsken, A.</creatorcontrib><creatorcontrib>Frey, B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Strahlentherapie und Onkologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hartmann, J.</au><au>Wölfelschneider, J.</au><au>Stache, C.</au><au>Buslei, R.</au><au>Derer, A.</au><au>Schwarz, M.</au><au>Bäuerle, T.</au><au>Fietkau, R.</au><au>Gaipl, U. S.</au><au>Bert, C.</au><au>Hölsken, A.</au><au>Frey, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel technique for high-precision stereotactic irradiation of mouse brains</atitle><jtitle>Strahlentherapie und Onkologie</jtitle><stitle>Strahlenther Onkol</stitle><addtitle>Strahlenther Onkol</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>192</volume><issue>11</issue><spage>806</spage><epage>814</epage><pages>806-814</pages><issn>0179-7158</issn><eissn>1439-099X</eissn><abstract>Background and purpose
Small animal irradiation systems were developed for preclinical evaluation of tumor therapy closely resembling the clinical situation. Mostly only clinical LINACs are available, so protocols for small animal partial body irradiation using a conventional clinical system are essential. This study defines a protocol for conformal brain tumor irradiations in mice.
Materials and methods
CT and MRI images were used to demarcate the target volume and organs at risk. Three 6 MV photon beams were planned for a total dose of 10 fractions of 1.8 Gy. The mouse position in a dedicated applicator was verified by an X‑ray patient positioning system before each irradiation. Dosimetric verifications (using ionization chambers and films) were performed. Irradiation-induced DNA damage was analyzed to verify the treatment effects on the cellular level.
Results
The defined treatment protocol and the applied fractionation scheme were feasible. The in-house developed applicator was suitable for individual positioning at submillimeter accuracy of anesthetized mice during irradiation, altogether performed in less than 10 min. All mice tolerated the treatment well. Measured dose values perfectly matched the nominal values from treatment planning. Cellular response was restricted to the target volume.
Conclusion
Clinical LINAC-based irradiations of mice offer the potential to treat orthotopic tumors conformably. Especially with respect to lateral penumbra, dedicated small animal irradiation systems exceed the clinical LINAC solution.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27402389</pmid><doi>10.1007/s00066-016-1014-8</doi><tpages>9</tpages></addata></record> |
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| ispartof | Strahlentherapie und Onkologie, 2016-11, Vol.192 (11), p.806-814 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Brain Neoplasms - diagnostic imaging Brain Neoplasms - radiotherapy Brain Neoplasms - veterinary Cell Line, Tumor Medicine Medicine & Public Health Mice Oncology Original Article Patient Positioning - veterinary Radiosurgery - veterinary Radiotherapy Radiotherapy Dosage Radiotherapy Planning, Computer-Assisted - veterinary Radiotherapy, Image-Guided - veterinary Reproducibility of Results Sensitivity and Specificity Treatment Outcome |
| title | Novel technique for high-precision stereotactic irradiation of mouse brains |
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