Constitutive expression of recombinant human hyaluronidase PH20 by Pichia pastoris

PH20 is known as sperm adhesion molecule 1 (SPAM1) and also has hyaluronidase function to preferentially hydrolyze the glycosidic linkage of hyaluronic acid (HA). A DNA fragment containing core domain of human PH20 gene was cloned into a constitutive expression plasmid (pGAPZαC) of Pichia pastoris t...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2016-12, Vol.122 (6), p.673-678
Hauptverfasser:	Chen, Kuan-Jung, Sabrina, Sabrina, El-Safory, Nermeen S., Lee, Guan-Chiun, Lee, Cheng-Kang
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Adhesion Molecules - genetics Cell Adhesion Molecules - metabolism Cloning, Molecular Functional expression Gene Expression Regulation, Fungal Glycoprotein Glycosylation Human hyaluronidase Humans Hyaluronic Acid - metabolism Hyaluronoglucosaminidase - genetics Hyaluronoglucosaminidase - metabolism Metabolic Engineering Molecular Weight Organisms, Genetically Modified PH20 Pichia - genetics Pichia - metabolism Pichia pastoris Recombinant Proteins - genetics Recombinant Proteins - metabolism Transfection
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