A dermal equivalent developed from adipose-derived stem cells and electrospun polycaprolactone matrix: an in vitro and in vivo study
Polycaprolactone (PCL) is used as a material of choice for surgical sutures, wound dressings, contraceptives, fixation devices and dentistry in paramedical sciences. In addition, adipose-derived stem cells (ASCs) have been shown to be effective in the treatment of acute and chronic wounds. This stud...
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| Veröffentlicht in: | Anatomical science international 2017-09, Vol.92 (4), p.509-520 |
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| creator | Bayati, Vahid Abbaspour, Mohammad Reza Dehbashi, Fereshteh Negad Neisi, Niloofar Hashemitabar, Mahmoud |
| description | Polycaprolactone (PCL) is used as a material of choice for surgical sutures, wound dressings, contraceptives, fixation devices and dentistry in paramedical sciences. In addition, adipose-derived stem cells (ASCs) have been shown to be effective in the treatment of acute and chronic wounds. This study aimed to evaluate the effect of electrospun PCL fibers on keratinocyte differentiation of ASCs and wound healing. PCL solution was electrospun and characterized. Isolated and characterized ASCs were differentiated into keratinocyte-like cells on a tissue culture plate (TCP) and PCL matrices and compared. PCL nano-/microfibers cultured with ASCs (test group) or alone (control) were implanted as a dermal substitute for wound healing. There were significant increases in the proliferation rate and expression level of
cytokeratin 14
,
filaggrin
and
involucrin
in cells cultured on PCL matrices compared to TCP (
p
|
| doi_str_mv | 10.1007/s12565-016-0352-z |
| format | Article |
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cytokeratin 14
,
filaggrin
and
involucrin
in cells cultured on PCL matrices compared to TCP (
p
< 0.05). After histological and immunological evaluation of the reconstituted skin, a thick epidermal layer with several skin appendages was evidently observed in the ASC/PCL group, whereas no real and mature epidermis was formed, especially in the central area of the healing wound in the pure PCL group on day 14. Pure PCL, if possessing suitable properties including good adhesiveness, high proliferative capability, inductive elasticity and stiffness for migration and differentiation, could drive the keratinocyte differentiation of ASCs and act as an efficient dermal equivalent to promote wound healing.</description><identifier>ISSN: 1447-6959</identifier><identifier>EISSN: 1447-073X</identifier><identifier>DOI: 10.1007/s12565-016-0352-z</identifier><identifier>PMID: 27329656</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Adipose Tissue - cytology ; Anatomy ; Animal Anatomy ; Animal Physiology ; Cell Biology ; Cell culture ; Cell Differentiation - drug effects ; Cells, Cultured ; Contraceptives ; Cytokeratin ; Dentistry ; Epidermis ; Epidermis - cytology ; Filaggrin ; Histology ; Human Physiology ; Humans ; Intermediate Filament Proteins - metabolism ; Keratin-14 - metabolism ; Keratinocytes - cytology ; Keratinocytes - metabolism ; Medical dressings ; Medicine ; Medicine & Public Health ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Morphology ; Neurosciences ; Original Article ; Polycaprolactone ; Polyesters - pharmacology ; Polyesters - therapeutic use ; Protein Precursors - metabolism ; Skin ; Skin Physiological Phenomena ; Solutions ; Stem cells ; Sutures ; Tissue culture ; Wound healing ; Wound Healing - drug effects ; Wound Healing - physiology ; Wounds and Injuries - therapy</subject><ispartof>Anatomical science international, 2017-09, Vol.92 (4), p.509-520</ispartof><rights>Japanese Association of Anatomists 2016</rights><rights>Copyright Springer Nature B.V. 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-def9cb5e90dd36d18087c8fe5fbb342745afae4c740412aecc81bb5e827989ce3</citedby><cites>FETCH-LOGICAL-c396t-def9cb5e90dd36d18087c8fe5fbb342745afae4c740412aecc81bb5e827989ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12565-016-0352-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12565-016-0352-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27329656$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bayati, Vahid</creatorcontrib><creatorcontrib>Abbaspour, Mohammad Reza</creatorcontrib><creatorcontrib>Dehbashi, Fereshteh Negad</creatorcontrib><creatorcontrib>Neisi, Niloofar</creatorcontrib><creatorcontrib>Hashemitabar, Mahmoud</creatorcontrib><title>A dermal equivalent developed from adipose-derived stem cells and electrospun polycaprolactone matrix: an in vitro and in vivo study</title><title>Anatomical science international</title><addtitle>Anat Sci Int</addtitle><addtitle>Anat Sci Int</addtitle><description>Polycaprolactone (PCL) is used as a material of choice for surgical sutures, wound dressings, contraceptives, fixation devices and dentistry in paramedical sciences. In addition, adipose-derived stem cells (ASCs) have been shown to be effective in the treatment of acute and chronic wounds. This study aimed to evaluate the effect of electrospun PCL fibers on keratinocyte differentiation of ASCs and wound healing. PCL solution was electrospun and characterized. Isolated and characterized ASCs were differentiated into keratinocyte-like cells on a tissue culture plate (TCP) and PCL matrices and compared. PCL nano-/microfibers cultured with ASCs (test group) or alone (control) were implanted as a dermal substitute for wound healing. There were significant increases in the proliferation rate and expression level of
cytokeratin 14
,
filaggrin
and
involucrin
in cells cultured on PCL matrices compared to TCP (
p
< 0.05). After histological and immunological evaluation of the reconstituted skin, a thick epidermal layer with several skin appendages was evidently observed in the ASC/PCL group, whereas no real and mature epidermis was formed, especially in the central area of the healing wound in the pure PCL group on day 14. Pure PCL, if possessing suitable properties including good adhesiveness, high proliferative capability, inductive elasticity and stiffness for migration and differentiation, could drive the keratinocyte differentiation of ASCs and act as an efficient dermal equivalent to promote wound healing.</description><subject>Adipose Tissue - cytology</subject><subject>Anatomy</subject><subject>Animal Anatomy</subject><subject>Animal Physiology</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Contraceptives</subject><subject>Cytokeratin</subject><subject>Dentistry</subject><subject>Epidermis</subject><subject>Epidermis - cytology</subject><subject>Filaggrin</subject><subject>Histology</subject><subject>Human Physiology</subject><subject>Humans</subject><subject>Intermediate Filament Proteins - metabolism</subject><subject>Keratin-14 - metabolism</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - metabolism</subject><subject>Medical dressings</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Morphology</subject><subject>Neurosciences</subject><subject>Original Article</subject><subject>Polycaprolactone</subject><subject>Polyesters - pharmacology</subject><subject>Polyesters - therapeutic use</subject><subject>Protein Precursors - metabolism</subject><subject>Skin</subject><subject>Skin Physiological Phenomena</subject><subject>Solutions</subject><subject>Stem cells</subject><subject>Sutures</subject><subject>Tissue culture</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><subject>Wound Healing - physiology</subject><subject>Wounds and Injuries - therapy</subject><issn>1447-6959</issn><issn>1447-073X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtr3TAQhUVJaNKkP6CbIMimGzd6WJKVXQh9QSCbFrITsjQODrLlSLbpzbo_vLq5tykUspI0-s6ZGQ5CHyj5RAlRF5kyIUVFqKwIF6x6eoOOaV2riih-d7C_Sy30EXqX8wMhVAvK36IjpjjTUshj9PsKe0iDDRgel361Aca5VFYIcQKPuxQHbH0_xQxVAfu1FPMMA3YQQsZ29BgCuDnFPC0jnmLYODulGKyb4wh4sHPqf10WEPcjXvsCPoueH2ssXovfnKLDzoYM7_fnCfr55fOP62_Vze3X79dXN5XjWs6lf6ddK0AT77n0tCGNck0HomtbXjNVC9tZqJ2qSU2ZBeca2ha-YUo32gE_QR93vmXAxwXybIY-bxexI8QlG9owqYgQNSvo-X_oQ1zSWKYzjFElhGy4LBTdUa7snxN0Zkr9YNPGUGK2EZldRKZEZLYRmaeiOds7L-0A_kXxN5MCsB2Qy9d4D-lf69dd_wDluZ-P</recordid><startdate>20170901</startdate><enddate>20170901</enddate><creator>Bayati, Vahid</creator><creator>Abbaspour, Mohammad Reza</creator><creator>Dehbashi, Fereshteh Negad</creator><creator>Neisi, Niloofar</creator><creator>Hashemitabar, Mahmoud</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20170901</creationdate><title>A dermal equivalent developed from adipose-derived stem cells and electrospun polycaprolactone matrix: an in vitro and in vivo study</title><author>Bayati, Vahid ; Abbaspour, Mohammad Reza ; Dehbashi, Fereshteh Negad ; Neisi, Niloofar ; Hashemitabar, Mahmoud</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-def9cb5e90dd36d18087c8fe5fbb342745afae4c740412aecc81bb5e827989ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adipose Tissue - cytology</topic><topic>Anatomy</topic><topic>Animal Anatomy</topic><topic>Animal Physiology</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Contraceptives</topic><topic>Cytokeratin</topic><topic>Dentistry</topic><topic>Epidermis</topic><topic>Epidermis - cytology</topic><topic>Filaggrin</topic><topic>Histology</topic><topic>Human Physiology</topic><topic>Humans</topic><topic>Intermediate Filament Proteins - metabolism</topic><topic>Keratin-14 - metabolism</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - metabolism</topic><topic>Medical dressings</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Morphology</topic><topic>Neurosciences</topic><topic>Original Article</topic><topic>Polycaprolactone</topic><topic>Polyesters - pharmacology</topic><topic>Polyesters - therapeutic use</topic><topic>Protein Precursors - metabolism</topic><topic>Skin</topic><topic>Skin Physiological Phenomena</topic><topic>Solutions</topic><topic>Stem cells</topic><topic>Sutures</topic><topic>Tissue culture</topic><topic>Wound healing</topic><topic>Wound Healing - drug effects</topic><topic>Wound Healing - physiology</topic><topic>Wounds and Injuries - therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bayati, Vahid</creatorcontrib><creatorcontrib>Abbaspour, Mohammad Reza</creatorcontrib><creatorcontrib>Dehbashi, Fereshteh Negad</creatorcontrib><creatorcontrib>Neisi, Niloofar</creatorcontrib><creatorcontrib>Hashemitabar, Mahmoud</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Anatomical science international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bayati, Vahid</au><au>Abbaspour, Mohammad Reza</au><au>Dehbashi, Fereshteh Negad</au><au>Neisi, Niloofar</au><au>Hashemitabar, Mahmoud</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A dermal equivalent developed from adipose-derived stem cells and electrospun polycaprolactone matrix: an in vitro and in vivo study</atitle><jtitle>Anatomical science international</jtitle><stitle>Anat Sci Int</stitle><addtitle>Anat Sci Int</addtitle><date>2017-09-01</date><risdate>2017</risdate><volume>92</volume><issue>4</issue><spage>509</spage><epage>520</epage><pages>509-520</pages><issn>1447-6959</issn><eissn>1447-073X</eissn><abstract>Polycaprolactone (PCL) is used as a material of choice for surgical sutures, wound dressings, contraceptives, fixation devices and dentistry in paramedical sciences. In addition, adipose-derived stem cells (ASCs) have been shown to be effective in the treatment of acute and chronic wounds. This study aimed to evaluate the effect of electrospun PCL fibers on keratinocyte differentiation of ASCs and wound healing. PCL solution was electrospun and characterized. Isolated and characterized ASCs were differentiated into keratinocyte-like cells on a tissue culture plate (TCP) and PCL matrices and compared. PCL nano-/microfibers cultured with ASCs (test group) or alone (control) were implanted as a dermal substitute for wound healing. There were significant increases in the proliferation rate and expression level of
cytokeratin 14
,
filaggrin
and
involucrin
in cells cultured on PCL matrices compared to TCP (
p
< 0.05). After histological and immunological evaluation of the reconstituted skin, a thick epidermal layer with several skin appendages was evidently observed in the ASC/PCL group, whereas no real and mature epidermis was formed, especially in the central area of the healing wound in the pure PCL group on day 14. Pure PCL, if possessing suitable properties including good adhesiveness, high proliferative capability, inductive elasticity and stiffness for migration and differentiation, could drive the keratinocyte differentiation of ASCs and act as an efficient dermal equivalent to promote wound healing.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>27329656</pmid><doi>10.1007/s12565-016-0352-z</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 1447-6959 |
| ispartof | Anatomical science international, 2017-09, Vol.92 (4), p.509-520 |
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| subjects | Adipose Tissue - cytology Anatomy Animal Anatomy Animal Physiology Cell Biology Cell culture Cell Differentiation - drug effects Cells, Cultured Contraceptives Cytokeratin Dentistry Epidermis Epidermis - cytology Filaggrin Histology Human Physiology Humans Intermediate Filament Proteins - metabolism Keratin-14 - metabolism Keratinocytes - cytology Keratinocytes - metabolism Medical dressings Medicine Medicine & Public Health Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Morphology Neurosciences Original Article Polycaprolactone Polyesters - pharmacology Polyesters - therapeutic use Protein Precursors - metabolism Skin Skin Physiological Phenomena Solutions Stem cells Sutures Tissue culture Wound healing Wound Healing - drug effects Wound Healing - physiology Wounds and Injuries - therapy |
| title | A dermal equivalent developed from adipose-derived stem cells and electrospun polycaprolactone matrix: an in vitro and in vivo study |
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