Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media
[Display omitted] •A novel fluorescent probe for singlet oxygen has been developed based on silica nanoparticles.•The nanoprobe is not affected by photosensitiser electrical charge or solvent polarity.•The nanoparticle prevents direct interactions with proteins and photosensitisers.•NanoADPA allows...
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| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2016-10, Vol.109, p.64-72 |
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| container_title | Methods (San Diego, Calif.) |
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| creator | Bresolí-Obach, Roger Nos, Jaume Mora, Margarita Sagristà, Maria Lluïsa Ruiz-González, Rubén Nonell, Santi |
| description | [Display omitted]
•A novel fluorescent probe for singlet oxygen has been developed based on silica nanoparticles.•The nanoprobe is not affected by photosensitiser electrical charge or solvent polarity.•The nanoparticle prevents direct interactions with proteins and photosensitisers.•NanoADPA allows detection of intracellular singlet oxygen.
We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen. |
| doi_str_mv | 10.1016/j.ymeth.2016.06.007 |
| format | Article |
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•A novel fluorescent probe for singlet oxygen has been developed based on silica nanoparticles.•The nanoprobe is not affected by photosensitiser electrical charge or solvent polarity.•The nanoparticle prevents direct interactions with proteins and photosensitisers.•NanoADPA allows detection of intracellular singlet oxygen.
We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.</description><identifier>ISSN: 1046-2023</identifier><identifier>EISSN: 1095-9130</identifier><identifier>DOI: 10.1016/j.ymeth.2016.06.007</identifier><identifier>PMID: 27302662</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anthracene dipropionic acid ; Anthracenes - chemistry ; Confocal microscopy ; Cytoplasm - chemistry ; Cytoplasm - metabolism ; Fluorescent Dyes - chemistry ; Fluorescent probe ; HeLa Cells ; Humans ; Nanoparticles - chemistry ; Silica nanoparticles ; Silicon Dioxide - chemistry ; Singlet oxygen ; Singlet Oxygen - chemistry ; Singlet Oxygen - isolation & purification</subject><ispartof>Methods (San Diego, Calif.), 2016-10, Vol.109, p.64-72</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-fb463cf2d68b540e2c6325ada7b91296566018adc415056acb918649c170654c3</citedby><cites>FETCH-LOGICAL-c359t-fb463cf2d68b540e2c6325ada7b91296566018adc415056acb918649c170654c3</cites><orcidid>0000-0002-8900-5291</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1046202316301712$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27302662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bresolí-Obach, Roger</creatorcontrib><creatorcontrib>Nos, Jaume</creatorcontrib><creatorcontrib>Mora, Margarita</creatorcontrib><creatorcontrib>Sagristà, Maria Lluïsa</creatorcontrib><creatorcontrib>Ruiz-González, Rubén</creatorcontrib><creatorcontrib>Nonell, Santi</creatorcontrib><title>Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media</title><title>Methods (San Diego, Calif.)</title><addtitle>Methods</addtitle><description>[Display omitted]
•A novel fluorescent probe for singlet oxygen has been developed based on silica nanoparticles.•The nanoprobe is not affected by photosensitiser electrical charge or solvent polarity.•The nanoparticle prevents direct interactions with proteins and photosensitisers.•NanoADPA allows detection of intracellular singlet oxygen.
We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.</description><subject>Anthracene dipropionic acid</subject><subject>Anthracenes - chemistry</subject><subject>Confocal microscopy</subject><subject>Cytoplasm - chemistry</subject><subject>Cytoplasm - metabolism</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent probe</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Nanoparticles - chemistry</subject><subject>Silica nanoparticles</subject><subject>Silicon Dioxide - chemistry</subject><subject>Singlet oxygen</subject><subject>Singlet Oxygen - chemistry</subject><subject>Singlet Oxygen - isolation & purification</subject><issn>1046-2023</issn><issn>1095-9130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEQx4MoVqufQJAcvWydJLvZ3YMHEV9Q8KLgLWSzs23KNqnJVuy3N7XVozAwD_7z-hFywWDCgMnrxWSzxGE-4SmZQDIoD8gJg7rIaibgcBvnMuPAxYicxrgAAMbL6piMeCmAS8lPyPutG-ZBG3SYNTpiS7t-7QPGVBmo086vgm8w0s4HGq2b9ThQ_7WZoaMtDmgG6x21jjbW935mje7pElurz8hRp_uI53s_Jm8P9693T9n05fH57naaGVHUQ9Y1uRSm462smiIH5EYKXuhWl03NeC0LKYFVujU5K6CQ2qRyJfPasBJkkRsxJle7uenOjzXGQS1tOr7vtUO_jopVXJbA6lIkqdhJTfAxBuzUKtilDhvFQG2RqoX6Qaq2SBUkgzJ1Xe4XrJv02V_PL8MkuNkJML35aTGoaCw6kyiExEe13v674BvAM4kJ</recordid><startdate>20161015</startdate><enddate>20161015</enddate><creator>Bresolí-Obach, Roger</creator><creator>Nos, Jaume</creator><creator>Mora, Margarita</creator><creator>Sagristà, Maria Lluïsa</creator><creator>Ruiz-González, Rubén</creator><creator>Nonell, Santi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8900-5291</orcidid></search><sort><creationdate>20161015</creationdate><title>Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media</title><author>Bresolí-Obach, Roger ; Nos, Jaume ; Mora, Margarita ; Sagristà, Maria Lluïsa ; Ruiz-González, Rubén ; Nonell, Santi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-fb463cf2d68b540e2c6325ada7b91296566018adc415056acb918649c170654c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anthracene dipropionic acid</topic><topic>Anthracenes - chemistry</topic><topic>Confocal microscopy</topic><topic>Cytoplasm - chemistry</topic><topic>Cytoplasm - metabolism</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent probe</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Nanoparticles - chemistry</topic><topic>Silica nanoparticles</topic><topic>Silicon Dioxide - chemistry</topic><topic>Singlet oxygen</topic><topic>Singlet Oxygen - chemistry</topic><topic>Singlet Oxygen - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bresolí-Obach, Roger</creatorcontrib><creatorcontrib>Nos, Jaume</creatorcontrib><creatorcontrib>Mora, Margarita</creatorcontrib><creatorcontrib>Sagristà, Maria Lluïsa</creatorcontrib><creatorcontrib>Ruiz-González, Rubén</creatorcontrib><creatorcontrib>Nonell, Santi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Methods (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bresolí-Obach, Roger</au><au>Nos, Jaume</au><au>Mora, Margarita</au><au>Sagristà, Maria Lluïsa</au><au>Ruiz-González, Rubén</au><au>Nonell, Santi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media</atitle><jtitle>Methods (San Diego, Calif.)</jtitle><addtitle>Methods</addtitle><date>2016-10-15</date><risdate>2016</risdate><volume>109</volume><spage>64</spage><epage>72</epage><pages>64-72</pages><issn>1046-2023</issn><eissn>1095-9130</eissn><abstract>[Display omitted]
•A novel fluorescent probe for singlet oxygen has been developed based on silica nanoparticles.•The nanoprobe is not affected by photosensitiser electrical charge or solvent polarity.•The nanoparticle prevents direct interactions with proteins and photosensitisers.•NanoADPA allows detection of intracellular singlet oxygen.
We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27302662</pmid><doi>10.1016/j.ymeth.2016.06.007</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-8900-5291</orcidid></addata></record> |
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| ispartof | Methods (San Diego, Calif.), 2016-10, Vol.109, p.64-72 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anthracene dipropionic acid Anthracenes - chemistry Confocal microscopy Cytoplasm - chemistry Cytoplasm - metabolism Fluorescent Dyes - chemistry Fluorescent probe HeLa Cells Humans Nanoparticles - chemistry Silica nanoparticles Silicon Dioxide - chemistry Singlet oxygen Singlet Oxygen - chemistry Singlet Oxygen - isolation & purification |
| title | Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media |
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