Protein Synthesis in Coupled and Uncoupled Cell-Free Prokaryotic Gene Expression Systems

Secondary structure formation of mRNA, caused by desynchronization of transcription and translation, is known to impact gene expression in vivo. Yet, inactivation of mRNA by secondary structures in cell-free protein expression is frequently overlooked. Transcription and translation rates are often n...

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Veröffentlicht in:	ACS synthetic biology 2016-12, Vol.5 (12), p.1433-1440
Hauptverfasser:	Hansen, Maike M. K, Ventosa Rosquelles, Marta, Yelleswarapu, Maaruthy, Maas, Roel J. M, van Vugt-Jonker, Aafke J, Heus, Hans A, Huck, Wilhelm T. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Regulation, Bacterial Nucleic Acid Conformation Protein Biosynthesis RNA, Messenger - genetics Transcription, Genetic
Online-Zugang:	Volltext
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