Fitness and competitive growth comparison of methicillin resistant and methicillin susceptible Staphylococcus aureus colonies
Exponential developments of both Methicillin resistant Staphylococcus aureus (MRSA) 3R ve 36R and methicillin susceptible Staphylococcus aureus (MSSA) 27S were evaluated in the presence and absence of oxacillin. The strains were isolated from the specimens collected in microbiology department. It wa...
Gespeichert in:
| Veröffentlicht in: | Microbial pathogenesis 2017-05, Vol.106, p.69-75 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 75 |
|---|---|
| container_issue | |
| container_start_page | 69 |
| container_title | Microbial pathogenesis |
| container_volume | 106 |
| creator | Durhan, Emine Korcan, Safiye Elif Altindis, Mustafa Konuk, Muhsin |
| description | Exponential developments of both Methicillin resistant Staphylococcus aureus (MRSA) 3R ve 36R and methicillin susceptible Staphylococcus aureus (MSSA) 27S were evaluated in the presence and absence of oxacillin. The strains were isolated from the specimens collected in microbiology department. It was also determined the transfer of mecA gene from 3R to 27S strain by using the replica plate technique. It was observed that the presence of antibiotics in the preliminary culture had a positive impact on the growth of the secondary culture of MRSA isolates. Comparison results of Rt bacteria in three different mixed cultures, assessed with Tukey’s HSD test, showed a significant statistical difference among the groups. The values were as following; on the first day; Df: 2, F: 60.90, P: 0.0001, second day; Df:2, F:90.56, P: 0.0000, and third day; Df:2, F:4.86, P:0.0557. As a result of the study, we can suggest that the gene expression levels of the transferred antibiotic resistance genes could help us in both controlling hospital originated sickness and developing new strategies to prevent the spread of resistant bacteria. |
| doi_str_mv | 10.1016/j.micpath.2016.06.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1826698578</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0882401016302650</els_id><sourcerecordid>1826698578</sourcerecordid><originalsourceid>FETCH-LOGICAL-c365t-fc5e59f7b26b0b54446a9509330c0cf8dc9b0a9bd55038367188441cdfb127ce3</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVpaTab_IQWH3vxZmRbtnQKITQfEMihyVnI43FXi225kpySQ_97ley25BYYGEbzzLyjl7EvHDYceH2224wWZxO3myKVG0gB6gNbcVB1zguQH9kKpCzyCjgcseMQdpCIqlSf2VHRFLKplFixP1c2ThRCZqYuQzfOFG20T5T99O533L4-GW-DmzLXZyPFrUU7DHbKPAUbopni6-jbTlgC0hxtO1D2I5p5-zw4dIhLUlk8pYRucJOlcMI-9WYIdHrIa_Z49f3h8ia_u7--vby4y7GsRcx7FCRU37RF3UIrqqqqjRKgyhIQsJcdqhaMajshoJRl3XApq4pj17e8aJDKNfu23zt792uhEPVo043DYCZyS9BcFnWtpGhkQsUeRe9C8NTr2dvR-GfNQb84r3f64Lx-cV5DinTKmn09SCztSN3_qX9WJ-B8D1D66JMlrwNampA66wmj7px9R-IvReubPQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1826698578</pqid></control><display><type>article</type><title>Fitness and competitive growth comparison of methicillin resistant and methicillin susceptible Staphylococcus aureus colonies</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Durhan, Emine ; Korcan, Safiye Elif ; Altindis, Mustafa ; Konuk, Muhsin</creator><creatorcontrib>Durhan, Emine ; Korcan, Safiye Elif ; Altindis, Mustafa ; Konuk, Muhsin</creatorcontrib><description>Exponential developments of both Methicillin resistant Staphylococcus aureus (MRSA) 3R ve 36R and methicillin susceptible Staphylococcus aureus (MSSA) 27S were evaluated in the presence and absence of oxacillin. The strains were isolated from the specimens collected in microbiology department. It was also determined the transfer of mecA gene from 3R to 27S strain by using the replica plate technique. It was observed that the presence of antibiotics in the preliminary culture had a positive impact on the growth of the secondary culture of MRSA isolates. Comparison results of Rt bacteria in three different mixed cultures, assessed with Tukey’s HSD test, showed a significant statistical difference among the groups. The values were as following; on the first day; Df: 2, F: 60.90, P: 0.0001, second day; Df:2, F:90.56, P: 0.0000, and third day; Df:2, F:4.86, P:0.0557. As a result of the study, we can suggest that the gene expression levels of the transferred antibiotic resistance genes could help us in both controlling hospital originated sickness and developing new strategies to prevent the spread of resistant bacteria.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2016.06.009</identifier><identifier>PMID: 27287495</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Bacterial Proteins - genetics ; Coculture Techniques ; Colony Count, Microbial ; Fitness cost ; Growth rate ; Humans ; mecA ; Methicillin - pharmacology ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - growth & development ; Microbial Sensitivity Tests - methods ; MRSA ; Oxacillin - pharmacology ; Penicillin-Binding Proteins - genetics ; Polymerase Chain Reaction ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - growth & development</subject><ispartof>Microbial pathogenesis, 2017-05, Vol.106, p.69-75</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-fc5e59f7b26b0b54446a9509330c0cf8dc9b0a9bd55038367188441cdfb127ce3</citedby><cites>FETCH-LOGICAL-c365t-fc5e59f7b26b0b54446a9509330c0cf8dc9b0a9bd55038367188441cdfb127ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2016.06.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27287495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Durhan, Emine</creatorcontrib><creatorcontrib>Korcan, Safiye Elif</creatorcontrib><creatorcontrib>Altindis, Mustafa</creatorcontrib><creatorcontrib>Konuk, Muhsin</creatorcontrib><title>Fitness and competitive growth comparison of methicillin resistant and methicillin susceptible Staphylococcus aureus colonies</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Exponential developments of both Methicillin resistant Staphylococcus aureus (MRSA) 3R ve 36R and methicillin susceptible Staphylococcus aureus (MSSA) 27S were evaluated in the presence and absence of oxacillin. The strains were isolated from the specimens collected in microbiology department. It was also determined the transfer of mecA gene from 3R to 27S strain by using the replica plate technique. It was observed that the presence of antibiotics in the preliminary culture had a positive impact on the growth of the secondary culture of MRSA isolates. Comparison results of Rt bacteria in three different mixed cultures, assessed with Tukey’s HSD test, showed a significant statistical difference among the groups. The values were as following; on the first day; Df: 2, F: 60.90, P: 0.0001, second day; Df:2, F:90.56, P: 0.0000, and third day; Df:2, F:4.86, P:0.0557. As a result of the study, we can suggest that the gene expression levels of the transferred antibiotic resistance genes could help us in both controlling hospital originated sickness and developing new strategies to prevent the spread of resistant bacteria.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Bacterial Proteins - genetics</subject><subject>Coculture Techniques</subject><subject>Colony Count, Microbial</subject><subject>Fitness cost</subject><subject>Growth rate</subject><subject>Humans</subject><subject>mecA</subject><subject>Methicillin - pharmacology</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - growth & development</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>MRSA</subject><subject>Oxacillin - pharmacology</subject><subject>Penicillin-Binding Proteins - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - growth & development</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpaTab_IQWH3vxZmRbtnQKITQfEMihyVnI43FXi225kpySQ_97ley25BYYGEbzzLyjl7EvHDYceH2224wWZxO3myKVG0gB6gNbcVB1zguQH9kKpCzyCjgcseMQdpCIqlSf2VHRFLKplFixP1c2ThRCZqYuQzfOFG20T5T99O533L4-GW-DmzLXZyPFrUU7DHbKPAUbopni6-jbTlgC0hxtO1D2I5p5-zw4dIhLUlk8pYRucJOlcMI-9WYIdHrIa_Z49f3h8ia_u7--vby4y7GsRcx7FCRU37RF3UIrqqqqjRKgyhIQsJcdqhaMajshoJRl3XApq4pj17e8aJDKNfu23zt792uhEPVo043DYCZyS9BcFnWtpGhkQsUeRe9C8NTr2dvR-GfNQb84r3f64Lx-cV5DinTKmn09SCztSN3_qX9WJ-B8D1D66JMlrwNampA66wmj7px9R-IvReubPQ</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Durhan, Emine</creator><creator>Korcan, Safiye Elif</creator><creator>Altindis, Mustafa</creator><creator>Konuk, Muhsin</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201705</creationdate><title>Fitness and competitive growth comparison of methicillin resistant and methicillin susceptible Staphylococcus aureus colonies</title><author>Durhan, Emine ; Korcan, Safiye Elif ; Altindis, Mustafa ; Konuk, Muhsin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-fc5e59f7b26b0b54446a9509330c0cf8dc9b0a9bd55038367188441cdfb127ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance</topic><topic>Bacterial Proteins - genetics</topic><topic>Coculture Techniques</topic><topic>Colony Count, Microbial</topic><topic>Fitness cost</topic><topic>Growth rate</topic><topic>Humans</topic><topic>mecA</topic><topic>Methicillin - pharmacology</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - growth & development</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>MRSA</topic><topic>Oxacillin - pharmacology</topic><topic>Penicillin-Binding Proteins - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Durhan, Emine</creatorcontrib><creatorcontrib>Korcan, Safiye Elif</creatorcontrib><creatorcontrib>Altindis, Mustafa</creatorcontrib><creatorcontrib>Konuk, Muhsin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Durhan, Emine</au><au>Korcan, Safiye Elif</au><au>Altindis, Mustafa</au><au>Konuk, Muhsin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fitness and competitive growth comparison of methicillin resistant and methicillin susceptible Staphylococcus aureus colonies</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2017-05</date><risdate>2017</risdate><volume>106</volume><spage>69</spage><epage>75</epage><pages>69-75</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>Exponential developments of both Methicillin resistant Staphylococcus aureus (MRSA) 3R ve 36R and methicillin susceptible Staphylococcus aureus (MSSA) 27S were evaluated in the presence and absence of oxacillin. The strains were isolated from the specimens collected in microbiology department. It was also determined the transfer of mecA gene from 3R to 27S strain by using the replica plate technique. It was observed that the presence of antibiotics in the preliminary culture had a positive impact on the growth of the secondary culture of MRSA isolates. Comparison results of Rt bacteria in three different mixed cultures, assessed with Tukey’s HSD test, showed a significant statistical difference among the groups. The values were as following; on the first day; Df: 2, F: 60.90, P: 0.0001, second day; Df:2, F:90.56, P: 0.0000, and third day; Df:2, F:4.86, P:0.0557. As a result of the study, we can suggest that the gene expression levels of the transferred antibiotic resistance genes could help us in both controlling hospital originated sickness and developing new strategies to prevent the spread of resistant bacteria.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>27287495</pmid><doi>10.1016/j.micpath.2016.06.009</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0882-4010 |
| ispartof | Microbial pathogenesis, 2017-05, Vol.106, p.69-75 |
| issn | 0882-4010 1096-1208 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1826698578 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Anti-Bacterial Agents - pharmacology Antibiotic resistance Bacterial Proteins - genetics Coculture Techniques Colony Count, Microbial Fitness cost Growth rate Humans mecA Methicillin - pharmacology Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - growth & development Microbial Sensitivity Tests - methods MRSA Oxacillin - pharmacology Penicillin-Binding Proteins - genetics Polymerase Chain Reaction Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - growth & development |
| title | Fitness and competitive growth comparison of methicillin resistant and methicillin susceptible Staphylococcus aureus colonies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T20%3A25%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fitness%20and%20competitive%20growth%20comparison%20of%20methicillin%20resistant%20and%20methicillin%20susceptible%20Staphylococcus%20aureus%20colonies&rft.jtitle=Microbial%20pathogenesis&rft.au=Durhan,%20Emine&rft.date=2017-05&rft.volume=106&rft.spage=69&rft.epage=75&rft.pages=69-75&rft.issn=0882-4010&rft.eissn=1096-1208&rft_id=info:doi/10.1016/j.micpath.2016.06.009&rft_dat=%3Cproquest_cross%3E1826698578%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1826698578&rft_id=info:pmid/27287495&rft_els_id=S0882401016302650&rfr_iscdi=true |