Expression of cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) in the lymphocytes of hyperplastic tonsils: comparison between allergic and nonallergic snoring children
Background Cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) are involved in allergic processes and play a role in adenotonsillar hyperplasia (AH). Clinically, only CysLTR1 may be blocked by montelukast. Our objective was to compare the expression of CysLTR1 and CysLTR2 in the B and T cel...
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| Veröffentlicht in: | International forum of allergy & rhinology 2016-11, Vol.6 (11), p.1151-1158 |
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| creator | Paulucci, Bruno Peres Pereira, Juliana Picciarelli, Patricia Levy, Debora Di Francesco, Renata Cantisani |
| description | Background
Cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) are involved in allergic processes and play a role in adenotonsillar hyperplasia (AH). Clinically, only CysLTR1 may be blocked by montelukast. Our objective was to compare the expression of CysLTR1 and CysLTR2 in the B and T cells of hyperplasic tonsils of sensitized (SE) and control (NS) snoring children.
Methods
Sixty children, 5 to 10 years of age, referred for adenotonsillectomy, were divided into SE and NS groups, according to their responses to the skin‐prick test. Cells from the removed tissues were stained for CysLTR1, CysLTR2, CD19, and CD3 and counted via flow cytometry. messenger RNA (mRNA) expression of the CysLTRs genes was measured real‐time quantitative reverse transcription polymerase chain reaction (RT‐qPCR).
Results
The SE group showed reduced expression of the small CD3+/CysLTR1+ lymphocytes (4.6 ± 2.2 vs 6.5 ± 5.0; p = 0.04). Regarding the large lymphocytes, the SE group showed lower expression of CD3+/CysLTR1+ (40.9 ± 14.5 vs 47.6 ± 11.7; p = 0.05), CD19+/CysLTR1+ (44.6 ± 16.9 vs 54.1 ± 12.4; p = 0.01), and CD19+/CysLTR2+ (55.3 ± 11.3 vs 61.5 ± 12.6; p = 0.05) lymphocytes. Considering the total number of lymphocytes, the SE group had fewer CD3+/CysLTR1+ lymphocytes (11.1 ± 5.5 vs 13.7 ± 6.2; p = 0.04). All other cell populations exhibited reduced expression in the SE group without statistical significance. The expression of CysLTR2 was significantly higher (p < 0.05) than CysLTR1 in most studied cell populations. The mRNA expression did not show significant differences between the groups.
Conclusion
The expression of CysLTR is higher in the lymphocytes of the NS children, and CysLTR2 shows greater expression than CysLTR1 Respiratory allergies do not appear to be a stimulus for AH occurrence. Newer drugs capable of blocking both CysLTRs warrant further study. |
| doi_str_mv | 10.1002/alr.21798 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1826689168</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1826689168</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3918-c99ba5abefbd442661a5333d7510a0563c0d94f3e0978ce326f72ec6663a75a43</originalsourceid><addsrcrecordid>eNp1kc9q3DAQh01paUKaQ1-gCHpJDk70x5Lt3sKSbAtLCyFtcxOyPM4qkSVXskn8XH3BautkD4XOZWbgm0-IX5a9J_iMYEzPlQ1nlJR19So7pLiguair4vV-LsVBdhzjPU7FCeekfJsd0JJSgit6mP2-fBoCxGi8Q75Deo4jGDdbZGF68GMw4AAF0DCMPiCClGsRRSerOW5urpd1mekpMg6NW0B27oet1_MIcafczgOEwao4Go1G76Kx8RPSvh9UMDE928D4COCQshbCXYJ2Uufdfo_OB-PukN4a2wZw77I3nbIRjp_7Ufb96vJm9TnffFt_WV1scs1qUuW6rhvFVQNd0xYFFYIozhhrS06wwlwwjdu66Bjguqw0MCq6koIWQjBVclWwo-xk8Q7B_5ogjrI3UYO1yoGfoiRVklY1EVVCP_6D3vsppC_sKMZZRUjBE3W6UDr4GAN0cgimV2GWBMtdmDKFKf-GmdgPz8ap6aHdky_RJeB8AR6Nhfn_JnmxuX5R5suFSSE_7S9UeJCiZCWXP7-u5Y_b9e2VWFGJ2R_6wLmf</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1835381145</pqid></control><display><type>article</type><title>Expression of cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) in the lymphocytes of hyperplastic tonsils: comparison between allergic and nonallergic snoring children</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Paulucci, Bruno Peres ; Pereira, Juliana ; Picciarelli, Patricia ; Levy, Debora ; Di Francesco, Renata Cantisani</creator><creatorcontrib>Paulucci, Bruno Peres ; Pereira, Juliana ; Picciarelli, Patricia ; Levy, Debora ; Di Francesco, Renata Cantisani</creatorcontrib><description>Background
Cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) are involved in allergic processes and play a role in adenotonsillar hyperplasia (AH). Clinically, only CysLTR1 may be blocked by montelukast. Our objective was to compare the expression of CysLTR1 and CysLTR2 in the B and T cells of hyperplasic tonsils of sensitized (SE) and control (NS) snoring children.
Methods
Sixty children, 5 to 10 years of age, referred for adenotonsillectomy, were divided into SE and NS groups, according to their responses to the skin‐prick test. Cells from the removed tissues were stained for CysLTR1, CysLTR2, CD19, and CD3 and counted via flow cytometry. messenger RNA (mRNA) expression of the CysLTRs genes was measured real‐time quantitative reverse transcription polymerase chain reaction (RT‐qPCR).
Results
The SE group showed reduced expression of the small CD3+/CysLTR1+ lymphocytes (4.6 ± 2.2 vs 6.5 ± 5.0; p = 0.04). Regarding the large lymphocytes, the SE group showed lower expression of CD3+/CysLTR1+ (40.9 ± 14.5 vs 47.6 ± 11.7; p = 0.05), CD19+/CysLTR1+ (44.6 ± 16.9 vs 54.1 ± 12.4; p = 0.01), and CD19+/CysLTR2+ (55.3 ± 11.3 vs 61.5 ± 12.6; p = 0.05) lymphocytes. Considering the total number of lymphocytes, the SE group had fewer CD3+/CysLTR1+ lymphocytes (11.1 ± 5.5 vs 13.7 ± 6.2; p = 0.04). All other cell populations exhibited reduced expression in the SE group without statistical significance. The expression of CysLTR2 was significantly higher (p < 0.05) than CysLTR1 in most studied cell populations. The mRNA expression did not show significant differences between the groups.
Conclusion
The expression of CysLTR is higher in the lymphocytes of the NS children, and CysLTR2 shows greater expression than CysLTR1 Respiratory allergies do not appear to be a stimulus for AH occurrence. Newer drugs capable of blocking both CysLTRs warrant further study.</description><identifier>ISSN: 2042-6976</identifier><identifier>EISSN: 2042-6984</identifier><identifier>DOI: 10.1002/alr.21798</identifier><identifier>PMID: 27221082</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adenoids - metabolism ; Adenoids - pathology ; allergy ; Child ; Child, Preschool ; cysteinyl leukotriene receptor ; Female ; Humans ; hyperplasia ; Hyperplasia - genetics ; Hyperplasia - metabolism ; Hypersensitivity - genetics ; Hypersensitivity - metabolism ; Hypersensitivity - pathology ; lymphocyte ; Lymphocytes - metabolism ; Male ; Palatine Tonsil - metabolism ; Palatine Tonsil - pathology ; Receptors, Leukotriene - genetics ; RNA, Messenger - metabolism ; Snoring - genetics ; Snoring - metabolism ; Snoring - pathology ; tonsil</subject><ispartof>International forum of allergy & rhinology, 2016-11, Vol.6 (11), p.1151-1158</ispartof><rights>2016 ARS‐AAOA, LLC</rights><rights>2016 ARS-AAOA, LLC.</rights><rights>2016 ARS-AAOA, LLC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3918-c99ba5abefbd442661a5333d7510a0563c0d94f3e0978ce326f72ec6663a75a43</citedby><cites>FETCH-LOGICAL-c3918-c99ba5abefbd442661a5333d7510a0563c0d94f3e0978ce326f72ec6663a75a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Falr.21798$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Falr.21798$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27221082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paulucci, Bruno Peres</creatorcontrib><creatorcontrib>Pereira, Juliana</creatorcontrib><creatorcontrib>Picciarelli, Patricia</creatorcontrib><creatorcontrib>Levy, Debora</creatorcontrib><creatorcontrib>Di Francesco, Renata Cantisani</creatorcontrib><title>Expression of cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) in the lymphocytes of hyperplastic tonsils: comparison between allergic and nonallergic snoring children</title><title>International forum of allergy & rhinology</title><addtitle>Int Forum Allergy Rhinol</addtitle><description>Background
Cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) are involved in allergic processes and play a role in adenotonsillar hyperplasia (AH). Clinically, only CysLTR1 may be blocked by montelukast. Our objective was to compare the expression of CysLTR1 and CysLTR2 in the B and T cells of hyperplasic tonsils of sensitized (SE) and control (NS) snoring children.
Methods
Sixty children, 5 to 10 years of age, referred for adenotonsillectomy, were divided into SE and NS groups, according to their responses to the skin‐prick test. Cells from the removed tissues were stained for CysLTR1, CysLTR2, CD19, and CD3 and counted via flow cytometry. messenger RNA (mRNA) expression of the CysLTRs genes was measured real‐time quantitative reverse transcription polymerase chain reaction (RT‐qPCR).
Results
The SE group showed reduced expression of the small CD3+/CysLTR1+ lymphocytes (4.6 ± 2.2 vs 6.5 ± 5.0; p = 0.04). Regarding the large lymphocytes, the SE group showed lower expression of CD3+/CysLTR1+ (40.9 ± 14.5 vs 47.6 ± 11.7; p = 0.05), CD19+/CysLTR1+ (44.6 ± 16.9 vs 54.1 ± 12.4; p = 0.01), and CD19+/CysLTR2+ (55.3 ± 11.3 vs 61.5 ± 12.6; p = 0.05) lymphocytes. Considering the total number of lymphocytes, the SE group had fewer CD3+/CysLTR1+ lymphocytes (11.1 ± 5.5 vs 13.7 ± 6.2; p = 0.04). All other cell populations exhibited reduced expression in the SE group without statistical significance. The expression of CysLTR2 was significantly higher (p < 0.05) than CysLTR1 in most studied cell populations. The mRNA expression did not show significant differences between the groups.
Conclusion
The expression of CysLTR is higher in the lymphocytes of the NS children, and CysLTR2 shows greater expression than CysLTR1 Respiratory allergies do not appear to be a stimulus for AH occurrence. Newer drugs capable of blocking both CysLTRs warrant further study.</description><subject>Adenoids - metabolism</subject><subject>Adenoids - pathology</subject><subject>allergy</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>cysteinyl leukotriene receptor</subject><subject>Female</subject><subject>Humans</subject><subject>hyperplasia</subject><subject>Hyperplasia - genetics</subject><subject>Hyperplasia - metabolism</subject><subject>Hypersensitivity - genetics</subject><subject>Hypersensitivity - metabolism</subject><subject>Hypersensitivity - pathology</subject><subject>lymphocyte</subject><subject>Lymphocytes - metabolism</subject><subject>Male</subject><subject>Palatine Tonsil - metabolism</subject><subject>Palatine Tonsil - pathology</subject><subject>Receptors, Leukotriene - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Snoring - genetics</subject><subject>Snoring - metabolism</subject><subject>Snoring - pathology</subject><subject>tonsil</subject><issn>2042-6976</issn><issn>2042-6984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9q3DAQh01paUKaQ1-gCHpJDk70x5Lt3sKSbAtLCyFtcxOyPM4qkSVXskn8XH3BautkD4XOZWbgm0-IX5a9J_iMYEzPlQ1nlJR19So7pLiguair4vV-LsVBdhzjPU7FCeekfJsd0JJSgit6mP2-fBoCxGi8Q75Deo4jGDdbZGF68GMw4AAF0DCMPiCClGsRRSerOW5urpd1mekpMg6NW0B27oet1_MIcafczgOEwao4Go1G76Kx8RPSvh9UMDE928D4COCQshbCXYJ2Uufdfo_OB-PukN4a2wZw77I3nbIRjp_7Ufb96vJm9TnffFt_WV1scs1qUuW6rhvFVQNd0xYFFYIozhhrS06wwlwwjdu66Bjguqw0MCq6koIWQjBVclWwo-xk8Q7B_5ogjrI3UYO1yoGfoiRVklY1EVVCP_6D3vsppC_sKMZZRUjBE3W6UDr4GAN0cgimV2GWBMtdmDKFKf-GmdgPz8ap6aHdky_RJeB8AR6Nhfn_JnmxuX5R5suFSSE_7S9UeJCiZCWXP7-u5Y_b9e2VWFGJ2R_6wLmf</recordid><startdate>201611</startdate><enddate>201611</enddate><creator>Paulucci, Bruno Peres</creator><creator>Pereira, Juliana</creator><creator>Picciarelli, Patricia</creator><creator>Levy, Debora</creator><creator>Di Francesco, Renata Cantisani</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201611</creationdate><title>Expression of cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) in the lymphocytes of hyperplastic tonsils: comparison between allergic and nonallergic snoring children</title><author>Paulucci, Bruno Peres ; Pereira, Juliana ; Picciarelli, Patricia ; Levy, Debora ; Di Francesco, Renata Cantisani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3918-c99ba5abefbd442661a5333d7510a0563c0d94f3e0978ce326f72ec6663a75a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adenoids - metabolism</topic><topic>Adenoids - pathology</topic><topic>allergy</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>cysteinyl leukotriene receptor</topic><topic>Female</topic><topic>Humans</topic><topic>hyperplasia</topic><topic>Hyperplasia - genetics</topic><topic>Hyperplasia - metabolism</topic><topic>Hypersensitivity - genetics</topic><topic>Hypersensitivity - metabolism</topic><topic>Hypersensitivity - pathology</topic><topic>lymphocyte</topic><topic>Lymphocytes - metabolism</topic><topic>Male</topic><topic>Palatine Tonsil - metabolism</topic><topic>Palatine Tonsil - pathology</topic><topic>Receptors, Leukotriene - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Snoring - genetics</topic><topic>Snoring - metabolism</topic><topic>Snoring - pathology</topic><topic>tonsil</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paulucci, Bruno Peres</creatorcontrib><creatorcontrib>Pereira, Juliana</creatorcontrib><creatorcontrib>Picciarelli, Patricia</creatorcontrib><creatorcontrib>Levy, Debora</creatorcontrib><creatorcontrib>Di Francesco, Renata Cantisani</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International forum of allergy & rhinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paulucci, Bruno Peres</au><au>Pereira, Juliana</au><au>Picciarelli, Patricia</au><au>Levy, Debora</au><au>Di Francesco, Renata Cantisani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) in the lymphocytes of hyperplastic tonsils: comparison between allergic and nonallergic snoring children</atitle><jtitle>International forum of allergy & rhinology</jtitle><addtitle>Int Forum Allergy Rhinol</addtitle><date>2016-11</date><risdate>2016</risdate><volume>6</volume><issue>11</issue><spage>1151</spage><epage>1158</epage><pages>1151-1158</pages><issn>2042-6976</issn><eissn>2042-6984</eissn><abstract>Background
Cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) are involved in allergic processes and play a role in adenotonsillar hyperplasia (AH). Clinically, only CysLTR1 may be blocked by montelukast. Our objective was to compare the expression of CysLTR1 and CysLTR2 in the B and T cells of hyperplasic tonsils of sensitized (SE) and control (NS) snoring children.
Methods
Sixty children, 5 to 10 years of age, referred for adenotonsillectomy, were divided into SE and NS groups, according to their responses to the skin‐prick test. Cells from the removed tissues were stained for CysLTR1, CysLTR2, CD19, and CD3 and counted via flow cytometry. messenger RNA (mRNA) expression of the CysLTRs genes was measured real‐time quantitative reverse transcription polymerase chain reaction (RT‐qPCR).
Results
The SE group showed reduced expression of the small CD3+/CysLTR1+ lymphocytes (4.6 ± 2.2 vs 6.5 ± 5.0; p = 0.04). Regarding the large lymphocytes, the SE group showed lower expression of CD3+/CysLTR1+ (40.9 ± 14.5 vs 47.6 ± 11.7; p = 0.05), CD19+/CysLTR1+ (44.6 ± 16.9 vs 54.1 ± 12.4; p = 0.01), and CD19+/CysLTR2+ (55.3 ± 11.3 vs 61.5 ± 12.6; p = 0.05) lymphocytes. Considering the total number of lymphocytes, the SE group had fewer CD3+/CysLTR1+ lymphocytes (11.1 ± 5.5 vs 13.7 ± 6.2; p = 0.04). All other cell populations exhibited reduced expression in the SE group without statistical significance. The expression of CysLTR2 was significantly higher (p < 0.05) than CysLTR1 in most studied cell populations. The mRNA expression did not show significant differences between the groups.
Conclusion
The expression of CysLTR is higher in the lymphocytes of the NS children, and CysLTR2 shows greater expression than CysLTR1 Respiratory allergies do not appear to be a stimulus for AH occurrence. Newer drugs capable of blocking both CysLTRs warrant further study.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>27221082</pmid><doi>10.1002/alr.21798</doi><tpages>8</tpages></addata></record> |
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| subjects | Adenoids - metabolism Adenoids - pathology allergy Child Child, Preschool cysteinyl leukotriene receptor Female Humans hyperplasia Hyperplasia - genetics Hyperplasia - metabolism Hypersensitivity - genetics Hypersensitivity - metabolism Hypersensitivity - pathology lymphocyte Lymphocytes - metabolism Male Palatine Tonsil - metabolism Palatine Tonsil - pathology Receptors, Leukotriene - genetics RNA, Messenger - metabolism Snoring - genetics Snoring - metabolism Snoring - pathology tonsil |
| title | Expression of cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) in the lymphocytes of hyperplastic tonsils: comparison between allergic and nonallergic snoring children |
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