Imaging flow cytometry for phytoplankton analysis
[Display omitted] •Imaging flow cytometry embraces various applications for phytoplankton analysis.•IFC combines power of flow cytometry and microscopy useful for phytoplankton study.•Available IFC instrumentation are variably exploited for different applications.•Examples of phytoplankton viability...
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| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2017-01, Vol.112, p.188-200 |
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| creator | Dashkova, Veronika Malashenkov, Dmitry Poulton, Nicole Vorobjev, Ivan Barteneva, Natasha S. |
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•Imaging flow cytometry embraces various applications for phytoplankton analysis.•IFC combines power of flow cytometry and microscopy useful for phytoplankton study.•Available IFC instrumentation are variably exploited for different applications.•Examples of phytoplankton viability and metabolic analysis using IFC are presented.
This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities’ composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments. |
| doi_str_mv | 10.1016/j.ymeth.2016.05.007 |
| format | Article |
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•Imaging flow cytometry embraces various applications for phytoplankton analysis.•IFC combines power of flow cytometry and microscopy useful for phytoplankton study.•Available IFC instrumentation are variably exploited for different applications.•Examples of phytoplankton viability and metabolic analysis using IFC are presented.
This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities’ composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments.</description><identifier>ISSN: 1046-2023</identifier><identifier>EISSN: 1095-9130</identifier><identifier>DOI: 10.1016/j.ymeth.2016.05.007</identifier><identifier>PMID: 27223402</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Chlorophyll - chemistry ; Cyanobacteria - metabolism ; Cyanobacteria - ultrastructure ; Flow Cytometry - methods ; Fluoresceins - chemistry ; Fluorescent Dyes - chemistry ; Harmful Algal Bloom - physiology ; Image Cytometry - methods ; Imaging flow cytometry ; Metabolic activity ; Microalgae ; Phytoplankton ; Phytoplankton - metabolism ; Phytoplankton - ultrastructure ; Staining and Labeling - methods ; Viability</subject><ispartof>Methods (San Diego, Calif.), 2017-01, Vol.112, p.188-200</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-e61e41158c8c4c9439a05c34e86e7d8799cdf143ccf22596f52b8a7f4bcc254d3</citedby><cites>FETCH-LOGICAL-c359t-e61e41158c8c4c9439a05c34e86e7d8799cdf143ccf22596f52b8a7f4bcc254d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S104620231630130X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27223402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dashkova, Veronika</creatorcontrib><creatorcontrib>Malashenkov, Dmitry</creatorcontrib><creatorcontrib>Poulton, Nicole</creatorcontrib><creatorcontrib>Vorobjev, Ivan</creatorcontrib><creatorcontrib>Barteneva, Natasha S.</creatorcontrib><title>Imaging flow cytometry for phytoplankton analysis</title><title>Methods (San Diego, Calif.)</title><addtitle>Methods</addtitle><description>[Display omitted]
•Imaging flow cytometry embraces various applications for phytoplankton analysis.•IFC combines power of flow cytometry and microscopy useful for phytoplankton study.•Available IFC instrumentation are variably exploited for different applications.•Examples of phytoplankton viability and metabolic analysis using IFC are presented.
This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities’ composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments.</description><subject>Chlorophyll - chemistry</subject><subject>Cyanobacteria - metabolism</subject><subject>Cyanobacteria - ultrastructure</subject><subject>Flow Cytometry - methods</subject><subject>Fluoresceins - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Harmful Algal Bloom - physiology</subject><subject>Image Cytometry - methods</subject><subject>Imaging flow cytometry</subject><subject>Metabolic activity</subject><subject>Microalgae</subject><subject>Phytoplankton</subject><subject>Phytoplankton - metabolism</subject><subject>Phytoplankton - ultrastructure</subject><subject>Staining and Labeling - methods</subject><subject>Viability</subject><issn>1046-2023</issn><issn>1095-9130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EoqXwBUgoSzYJfsdesEAVj0pIbGBtuY7TuiRxsFNQ_h6XFpasZq50Z-7MAeASwQJBxG82xdjaYV3gJArICgjLIzBFULJcIgKPdz3lOYaYTMBZjBsIIcKlOAUTXGJMKMRTgBatXrluldWN_8rMOPi0M4xZ7UPWr5PsG929D77LdKebMbp4Dk5q3UR7cagz8PZw_zp_yp9fHhfzu-fcECaH3HJkKUJMGGGokZRIDZkh1Apuy0qUUpqqRpQYU2PMJK8ZXgpd1nRpDGa0IjNwvd_bB_-xtXFQrYvGNuke67dRIYE5F1wKnKxkbzXBxxhsrfrgWh1GhaDasVIb9cNK7VgpyFRilaauDgHbZWurv5lfOMlwuzfY9Oans0FF42xnbOWCNYOqvPs34BseIHtg</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Dashkova, Veronika</creator><creator>Malashenkov, Dmitry</creator><creator>Poulton, Nicole</creator><creator>Vorobjev, Ivan</creator><creator>Barteneva, Natasha S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170101</creationdate><title>Imaging flow cytometry for phytoplankton analysis</title><author>Dashkova, Veronika ; Malashenkov, Dmitry ; Poulton, Nicole ; Vorobjev, Ivan ; Barteneva, Natasha S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-e61e41158c8c4c9439a05c34e86e7d8799cdf143ccf22596f52b8a7f4bcc254d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Chlorophyll - chemistry</topic><topic>Cyanobacteria - metabolism</topic><topic>Cyanobacteria - ultrastructure</topic><topic>Flow Cytometry - methods</topic><topic>Fluoresceins - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Harmful Algal Bloom - physiology</topic><topic>Image Cytometry - methods</topic><topic>Imaging flow cytometry</topic><topic>Metabolic activity</topic><topic>Microalgae</topic><topic>Phytoplankton</topic><topic>Phytoplankton - metabolism</topic><topic>Phytoplankton - ultrastructure</topic><topic>Staining and Labeling - methods</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dashkova, Veronika</creatorcontrib><creatorcontrib>Malashenkov, Dmitry</creatorcontrib><creatorcontrib>Poulton, Nicole</creatorcontrib><creatorcontrib>Vorobjev, Ivan</creatorcontrib><creatorcontrib>Barteneva, Natasha S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Methods (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dashkova, Veronika</au><au>Malashenkov, Dmitry</au><au>Poulton, Nicole</au><au>Vorobjev, Ivan</au><au>Barteneva, Natasha S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imaging flow cytometry for phytoplankton analysis</atitle><jtitle>Methods (San Diego, Calif.)</jtitle><addtitle>Methods</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>112</volume><spage>188</spage><epage>200</epage><pages>188-200</pages><issn>1046-2023</issn><eissn>1095-9130</eissn><abstract>[Display omitted]
•Imaging flow cytometry embraces various applications for phytoplankton analysis.•IFC combines power of flow cytometry and microscopy useful for phytoplankton study.•Available IFC instrumentation are variably exploited for different applications.•Examples of phytoplankton viability and metabolic analysis using IFC are presented.
This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities’ composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27223402</pmid><doi>10.1016/j.ymeth.2016.05.007</doi><tpages>13</tpages></addata></record> |
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| ispartof | Methods (San Diego, Calif.), 2017-01, Vol.112, p.188-200 |
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| subjects | Chlorophyll - chemistry Cyanobacteria - metabolism Cyanobacteria - ultrastructure Flow Cytometry - methods Fluoresceins - chemistry Fluorescent Dyes - chemistry Harmful Algal Bloom - physiology Image Cytometry - methods Imaging flow cytometry Metabolic activity Microalgae Phytoplankton Phytoplankton - metabolism Phytoplankton - ultrastructure Staining and Labeling - methods Viability |
| title | Imaging flow cytometry for phytoplankton analysis |
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