Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model
Objective: The aim of this study was to evaluate the effectiveness of the application of cultured autologous bone marrow mesenchymal stem cells (BMSCs) with scaffold and low-level laser therapy (LLLT) on the repair of articular cartilage defects in rabbits. Background data: For healing of the articu...
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| Veröffentlicht in: | Photomedicine and laser surgery 2016-11, Vol.34 (11), p.543-549 |
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| creator | Fekrazad, Reza Eslaminejad, Mohamadreza Baghaban Shayan, Arman M. Kalhori, Katayoun A.M. Abbas, Fatemeh Mashhadi Taghiyar, Leila Sepehr Pedram, Mir Ghuchani, Mostafa Sadeghi |
| description | Objective:
The aim of this study was to evaluate the effectiveness of the application of cultured autologous bone marrow mesenchymal stem cells (BMSCs) with scaffold and low-level laser therapy (LLLT) on the repair of articular cartilage defects in rabbits.
Background data:
For healing of the articular cartilage defects, although positive effects of BMSCs and LLLT have been demonstrated, their combination effect is still unknown; therefore, we investigated combining these two techniques has a synergistic effect.
Materials and methods:
After bone marrow aspiration from 10 rabbits, BMSCs were isolated, cultured in monolayer, suspended on a type I collagen scaffold and then implanted onto a full-thickness osteochondral defect (4 mm in diameter), artificially made on the patellar groove of both knees in the same rabbits. Then a knee was selected randomly in each rabbit as the experimental group, and subjected to Ga-Al-As (810 nm) laser irradiation with energy density of 4 J/cm
2
every other day for 3 weeks. As the control group, the other knee did not receive LLLT. After this period, animals were euthanized and osteochondral defects were evaluated by histomorphometric methods.
Results:
No significant difference in new cartilage formation and inflammation was found between the groups (
p >
0.05). However, there was significantly more new bone formation in the experimental group (
p <
0.05).
Conclusions:
In terms of our research, although better healing in osteochondral defects was seen when combining BMSCs and LLLT compared with the use of BMSCs alone, this improvement was predominantly caused by new bone formation rather than new cartilage formation. |
| doi_str_mv | 10.1089/pho.2015.4028 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1826686262</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1826686262</sourcerecordid><originalsourceid>FETCH-LOGICAL-c337t-118a2f644bfe04c70357d2fef2cc157424fa4762a3b2702bfa6584578d7a04a93</originalsourceid><addsrcrecordid>eNqFkD1PwzAQhi0EglIYWZFHlhTbsWNnRKF8SK1AfMyR45ypURKX2B3497gqIDamO52ee3X3IHRGyYwSVV6uV37GCBUzTpjaQxMqhMyUEGT_T3-EjkN4J4SVsswP0RGTRChCywlyc2vBxIC9xY8rH33jfO_bTaej8wPWQ4uXEGAwq89ed_g5Qo8r6Lq0MOCrMTqT0BFXOrWdfgN8Dbs8l5bxk24aF_HSt9CdoAOruwCn33WKXm_mL9Vdtni4va-uFpnJcxkzSpVmtuC8sUC4kSQXsmUWLDOGCskZt5rLgum8SV-wxupCKC6kaqUmXJf5FF3sctej_9hAiHXvgkkn6wH8JtRUsaJQBStYQrMdakYfwgi2Xo-u1-NnTUm9tVsnu_XWbr21m_jz7-hN00P7S__oTEC-A7ZjPQydgwbG-E_sF8xbhqI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1826686262</pqid></control><display><type>article</type><title>Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Fekrazad, Reza ; Eslaminejad, Mohamadreza Baghaban ; Shayan, Arman M. ; Kalhori, Katayoun A.M. ; Abbas, Fatemeh Mashhadi ; Taghiyar, Leila ; Sepehr Pedram, Mir ; Ghuchani, Mostafa Sadeghi</creator><creatorcontrib>Fekrazad, Reza ; Eslaminejad, Mohamadreza Baghaban ; Shayan, Arman M. ; Kalhori, Katayoun A.M. ; Abbas, Fatemeh Mashhadi ; Taghiyar, Leila ; Sepehr Pedram, Mir ; Ghuchani, Mostafa Sadeghi</creatorcontrib><description>Objective:
The aim of this study was to evaluate the effectiveness of the application of cultured autologous bone marrow mesenchymal stem cells (BMSCs) with scaffold and low-level laser therapy (LLLT) on the repair of articular cartilage defects in rabbits.
Background data:
For healing of the articular cartilage defects, although positive effects of BMSCs and LLLT have been demonstrated, their combination effect is still unknown; therefore, we investigated combining these two techniques has a synergistic effect.
Materials and methods:
After bone marrow aspiration from 10 rabbits, BMSCs were isolated, cultured in monolayer, suspended on a type I collagen scaffold and then implanted onto a full-thickness osteochondral defect (4 mm in diameter), artificially made on the patellar groove of both knees in the same rabbits. Then a knee was selected randomly in each rabbit as the experimental group, and subjected to Ga-Al-As (810 nm) laser irradiation with energy density of 4 J/cm
2
every other day for 3 weeks. As the control group, the other knee did not receive LLLT. After this period, animals were euthanized and osteochondral defects were evaluated by histomorphometric methods.
Results:
No significant difference in new cartilage formation and inflammation was found between the groups (
p >
0.05). However, there was significantly more new bone formation in the experimental group (
p <
0.05).
Conclusions:
In terms of our research, although better healing in osteochondral defects was seen when combining BMSCs and LLLT compared with the use of BMSCs alone, this improvement was predominantly caused by new bone formation rather than new cartilage formation.</description><identifier>ISSN: 1557-8550</identifier><identifier>EISSN: 1557-8550</identifier><identifier>DOI: 10.1089/pho.2015.4028</identifier><identifier>PMID: 27058019</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Analysis of Variance ; Animals ; Biopsy, Needle ; Bone Marrow Cells - radiation effects ; Bone Marrow Transplantation - methods ; Cartilage, Articular - injuries ; Cartilage, Articular - pathology ; Cartilage, Articular - radiation effects ; Cells, Cultured ; Disease Models, Animal ; Immunohistochemistry ; Knee Joint - pathology ; Knee Joint - radiation effects ; Low-Level Light Therapy - methods ; Male ; Mesenchymal Stromal Cells - radiation effects ; Original Research ; Rabbits ; Random Allocation ; Regeneration - physiology ; Regeneration - radiation effects ; Treatment Outcome</subject><ispartof>Photomedicine and laser surgery, 2016-11, Vol.34 (11), p.543-549</ispartof><rights>2016, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-118a2f644bfe04c70357d2fef2cc157424fa4762a3b2702bfa6584578d7a04a93</citedby><cites>FETCH-LOGICAL-c337t-118a2f644bfe04c70357d2fef2cc157424fa4762a3b2702bfa6584578d7a04a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27058019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fekrazad, Reza</creatorcontrib><creatorcontrib>Eslaminejad, Mohamadreza Baghaban</creatorcontrib><creatorcontrib>Shayan, Arman M.</creatorcontrib><creatorcontrib>Kalhori, Katayoun A.M.</creatorcontrib><creatorcontrib>Abbas, Fatemeh Mashhadi</creatorcontrib><creatorcontrib>Taghiyar, Leila</creatorcontrib><creatorcontrib>Sepehr Pedram, Mir</creatorcontrib><creatorcontrib>Ghuchani, Mostafa Sadeghi</creatorcontrib><title>Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model</title><title>Photomedicine and laser surgery</title><addtitle>Photomed Laser Surg</addtitle><description>Objective:
The aim of this study was to evaluate the effectiveness of the application of cultured autologous bone marrow mesenchymal stem cells (BMSCs) with scaffold and low-level laser therapy (LLLT) on the repair of articular cartilage defects in rabbits.
Background data:
For healing of the articular cartilage defects, although positive effects of BMSCs and LLLT have been demonstrated, their combination effect is still unknown; therefore, we investigated combining these two techniques has a synergistic effect.
Materials and methods:
After bone marrow aspiration from 10 rabbits, BMSCs were isolated, cultured in monolayer, suspended on a type I collagen scaffold and then implanted onto a full-thickness osteochondral defect (4 mm in diameter), artificially made on the patellar groove of both knees in the same rabbits. Then a knee was selected randomly in each rabbit as the experimental group, and subjected to Ga-Al-As (810 nm) laser irradiation with energy density of 4 J/cm
2
every other day for 3 weeks. As the control group, the other knee did not receive LLLT. After this period, animals were euthanized and osteochondral defects were evaluated by histomorphometric methods.
Results:
No significant difference in new cartilage formation and inflammation was found between the groups (
p >
0.05). However, there was significantly more new bone formation in the experimental group (
p <
0.05).
Conclusions:
In terms of our research, although better healing in osteochondral defects was seen when combining BMSCs and LLLT compared with the use of BMSCs alone, this improvement was predominantly caused by new bone formation rather than new cartilage formation.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Biopsy, Needle</subject><subject>Bone Marrow Cells - radiation effects</subject><subject>Bone Marrow Transplantation - methods</subject><subject>Cartilage, Articular - injuries</subject><subject>Cartilage, Articular - pathology</subject><subject>Cartilage, Articular - radiation effects</subject><subject>Cells, Cultured</subject><subject>Disease Models, Animal</subject><subject>Immunohistochemistry</subject><subject>Knee Joint - pathology</subject><subject>Knee Joint - radiation effects</subject><subject>Low-Level Light Therapy - methods</subject><subject>Male</subject><subject>Mesenchymal Stromal Cells - radiation effects</subject><subject>Original Research</subject><subject>Rabbits</subject><subject>Random Allocation</subject><subject>Regeneration - physiology</subject><subject>Regeneration - radiation effects</subject><subject>Treatment Outcome</subject><issn>1557-8550</issn><issn>1557-8550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EglIYWZFHlhTbsWNnRKF8SK1AfMyR45ypURKX2B3497gqIDamO52ee3X3IHRGyYwSVV6uV37GCBUzTpjaQxMqhMyUEGT_T3-EjkN4J4SVsswP0RGTRChCywlyc2vBxIC9xY8rH33jfO_bTaej8wPWQ4uXEGAwq89ed_g5Qo8r6Lq0MOCrMTqT0BFXOrWdfgN8Dbs8l5bxk24aF_HSt9CdoAOruwCn33WKXm_mL9Vdtni4va-uFpnJcxkzSpVmtuC8sUC4kSQXsmUWLDOGCskZt5rLgum8SV-wxupCKC6kaqUmXJf5FF3sctej_9hAiHXvgkkn6wH8JtRUsaJQBStYQrMdakYfwgi2Xo-u1-NnTUm9tVsnu_XWbr21m_jz7-hN00P7S__oTEC-A7ZjPQydgwbG-E_sF8xbhqI</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Fekrazad, Reza</creator><creator>Eslaminejad, Mohamadreza Baghaban</creator><creator>Shayan, Arman M.</creator><creator>Kalhori, Katayoun A.M.</creator><creator>Abbas, Fatemeh Mashhadi</creator><creator>Taghiyar, Leila</creator><creator>Sepehr Pedram, Mir</creator><creator>Ghuchani, Mostafa Sadeghi</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20161101</creationdate><title>Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model</title><author>Fekrazad, Reza ; Eslaminejad, Mohamadreza Baghaban ; Shayan, Arman M. ; Kalhori, Katayoun A.M. ; Abbas, Fatemeh Mashhadi ; Taghiyar, Leila ; Sepehr Pedram, Mir ; Ghuchani, Mostafa Sadeghi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-118a2f644bfe04c70357d2fef2cc157424fa4762a3b2702bfa6584578d7a04a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Biopsy, Needle</topic><topic>Bone Marrow Cells - radiation effects</topic><topic>Bone Marrow Transplantation - methods</topic><topic>Cartilage, Articular - injuries</topic><topic>Cartilage, Articular - pathology</topic><topic>Cartilage, Articular - radiation effects</topic><topic>Cells, Cultured</topic><topic>Disease Models, Animal</topic><topic>Immunohistochemistry</topic><topic>Knee Joint - pathology</topic><topic>Knee Joint - radiation effects</topic><topic>Low-Level Light Therapy - methods</topic><topic>Male</topic><topic>Mesenchymal Stromal Cells - radiation effects</topic><topic>Original Research</topic><topic>Rabbits</topic><topic>Random Allocation</topic><topic>Regeneration - physiology</topic><topic>Regeneration - radiation effects</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fekrazad, Reza</creatorcontrib><creatorcontrib>Eslaminejad, Mohamadreza Baghaban</creatorcontrib><creatorcontrib>Shayan, Arman M.</creatorcontrib><creatorcontrib>Kalhori, Katayoun A.M.</creatorcontrib><creatorcontrib>Abbas, Fatemeh Mashhadi</creatorcontrib><creatorcontrib>Taghiyar, Leila</creatorcontrib><creatorcontrib>Sepehr Pedram, Mir</creatorcontrib><creatorcontrib>Ghuchani, Mostafa Sadeghi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photomedicine and laser surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fekrazad, Reza</au><au>Eslaminejad, Mohamadreza Baghaban</au><au>Shayan, Arman M.</au><au>Kalhori, Katayoun A.M.</au><au>Abbas, Fatemeh Mashhadi</au><au>Taghiyar, Leila</au><au>Sepehr Pedram, Mir</au><au>Ghuchani, Mostafa Sadeghi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model</atitle><jtitle>Photomedicine and laser surgery</jtitle><addtitle>Photomed Laser Surg</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>34</volume><issue>11</issue><spage>543</spage><epage>549</epage><pages>543-549</pages><issn>1557-8550</issn><eissn>1557-8550</eissn><abstract>Objective:
The aim of this study was to evaluate the effectiveness of the application of cultured autologous bone marrow mesenchymal stem cells (BMSCs) with scaffold and low-level laser therapy (LLLT) on the repair of articular cartilage defects in rabbits.
Background data:
For healing of the articular cartilage defects, although positive effects of BMSCs and LLLT have been demonstrated, their combination effect is still unknown; therefore, we investigated combining these two techniques has a synergistic effect.
Materials and methods:
After bone marrow aspiration from 10 rabbits, BMSCs were isolated, cultured in monolayer, suspended on a type I collagen scaffold and then implanted onto a full-thickness osteochondral defect (4 mm in diameter), artificially made on the patellar groove of both knees in the same rabbits. Then a knee was selected randomly in each rabbit as the experimental group, and subjected to Ga-Al-As (810 nm) laser irradiation with energy density of 4 J/cm
2
every other day for 3 weeks. As the control group, the other knee did not receive LLLT. After this period, animals were euthanized and osteochondral defects were evaluated by histomorphometric methods.
Results:
No significant difference in new cartilage formation and inflammation was found between the groups (
p >
0.05). However, there was significantly more new bone formation in the experimental group (
p <
0.05).
Conclusions:
In terms of our research, although better healing in osteochondral defects was seen when combining BMSCs and LLLT compared with the use of BMSCs alone, this improvement was predominantly caused by new bone formation rather than new cartilage formation.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>27058019</pmid><doi>10.1089/pho.2015.4028</doi><tpages>7</tpages></addata></record> |
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| ispartof | Photomedicine and laser surgery, 2016-11, Vol.34 (11), p.543-549 |
| issn | 1557-8550 1557-8550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1826686262 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Analysis of Variance Animals Biopsy, Needle Bone Marrow Cells - radiation effects Bone Marrow Transplantation - methods Cartilage, Articular - injuries Cartilage, Articular - pathology Cartilage, Articular - radiation effects Cells, Cultured Disease Models, Animal Immunohistochemistry Knee Joint - pathology Knee Joint - radiation effects Low-Level Light Therapy - methods Male Mesenchymal Stromal Cells - radiation effects Original Research Rabbits Random Allocation Regeneration - physiology Regeneration - radiation effects Treatment Outcome |
| title | Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model |
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