Augmenting the bioactivity of polyetheretherketone using a novel accelerated neutral atom beam technique
Polyetheretherketone (PEEK) is an alternative to metallic implants in orthopedic applications; however, PEEK is bioinert and does not osteointegrate. In this study, an accelerated neutral atom beam technique (ANAB) was employed to improve the bioactivity of PEEK. The aim was to investigate the growt...
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| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2017-08, Vol.105 (6), p.1438-1446 |
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| creator | Ajami, S Coathup, M J Khoury, J Blunn, G W |
| description | Polyetheretherketone (PEEK) is an alternative to metallic implants in orthopedic applications; however, PEEK is bioinert and does not osteointegrate. In this study, an accelerated neutral atom beam technique (ANAB) was employed to improve the bioactivity of PEEK. The aim was to investigate the growth of human mesenchymal stem cells (hMSCs), human osteoblasts (hOB), and skin fibroblasts (BR3G) on PEEK and ANAB PEEK.
The surface roughness and contact angle of PEEK and ANAB PEEK was measured. Cell metabolic activity, proliferation and alkaline phosphatase (ALP) was measured and cell attachment was determined by quantifying adhesion plaques with cells.
ANAB treatment increased the surface hydrophilicity [91.74 ± 4.80° (PEEK) vs. 74.82 ± 2.70° (ANAB PEEK), p |
| doi_str_mv | 10.1002/jbm.b.33681 |
| format | Article |
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The surface roughness and contact angle of PEEK and ANAB PEEK was measured. Cell metabolic activity, proliferation and alkaline phosphatase (ALP) was measured and cell attachment was determined by quantifying adhesion plaques with cells.
ANAB treatment increased the surface hydrophilicity [91.74 ± 4.80° (PEEK) vs. 74.82 ± 2.70° (ANAB PEEK), p < 0.001] but did not alter the surface roughness. Metabolic activity and proliferation for all cell types significantly increased on ANAB PEEK compared to PEEK (p < 0.05). Significantly increased cell attachment was measured on ANAB PEEK surfaces. MSCs seeded on ANAB PEEK in the presence of osteogenic media, expressed increased levels of ALP compared to untreated PEEK (p < 0.05) CONCLUSION: Our results demonstrated that ANAB treatment increased the cell attachment, metabolic activity, and proliferation on PEEK. ANAB treatment may improve the osteointegration of PEEK implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1438-1446, 2017.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.33681</identifier><identifier>PMID: 27086858</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adhesion tests ; Alkaline phosphatase ; Attachment ; Biochemistry ; Biocompatibility ; Biological activity ; Biomedical materials ; Bone implants ; Bone Substitutes - chemistry ; Cell Adhesion ; Cell Line, Transformed ; Contact angle ; Fibroblasts ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Humans ; Ketones - chemistry ; Materials research ; Materials science ; Materials Testing ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Mesenchyme ; Metabolism ; Osseointegration ; Osteoblasts ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Plaques ; Polyether ether ketones ; Polyethylene Glycols - chemistry ; Skin ; Stem cells ; Surface chemistry ; Surface roughness ; Surgical implants</subject><ispartof>Journal of biomedical materials research. Part B, Applied biomaterials, 2017-08, Vol.105 (6), p.1438-1446</ispartof><rights>2016 Wiley Periodicals, Inc.</rights><rights>2017 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-6b909f62e34657502d65a65743ee24f7f08859bdb9cd9379f090972d5931f3</citedby><cites>FETCH-LOGICAL-c354t-6b909f62e34657502d65a65743ee24f7f08859bdb9cd9379f090972d5931f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27086858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ajami, S</creatorcontrib><creatorcontrib>Coathup, M J</creatorcontrib><creatorcontrib>Khoury, J</creatorcontrib><creatorcontrib>Blunn, G W</creatorcontrib><title>Augmenting the bioactivity of polyetheretherketone using a novel accelerated neutral atom beam technique</title><title>Journal of biomedical materials research. Part B, Applied biomaterials</title><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><description>Polyetheretherketone (PEEK) is an alternative to metallic implants in orthopedic applications; however, PEEK is bioinert and does not osteointegrate. In this study, an accelerated neutral atom beam technique (ANAB) was employed to improve the bioactivity of PEEK. The aim was to investigate the growth of human mesenchymal stem cells (hMSCs), human osteoblasts (hOB), and skin fibroblasts (BR3G) on PEEK and ANAB PEEK.
The surface roughness and contact angle of PEEK and ANAB PEEK was measured. Cell metabolic activity, proliferation and alkaline phosphatase (ALP) was measured and cell attachment was determined by quantifying adhesion plaques with cells.
ANAB treatment increased the surface hydrophilicity [91.74 ± 4.80° (PEEK) vs. 74.82 ± 2.70° (ANAB PEEK), p < 0.001] but did not alter the surface roughness. Metabolic activity and proliferation for all cell types significantly increased on ANAB PEEK compared to PEEK (p < 0.05). Significantly increased cell attachment was measured on ANAB PEEK surfaces. MSCs seeded on ANAB PEEK in the presence of osteogenic media, expressed increased levels of ALP compared to untreated PEEK (p < 0.05) CONCLUSION: Our results demonstrated that ANAB treatment increased the cell attachment, metabolic activity, and proliferation on PEEK. ANAB treatment may improve the osteointegration of PEEK implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1438-1446, 2017.</description><subject>Adhesion tests</subject><subject>Alkaline phosphatase</subject><subject>Attachment</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biological activity</subject><subject>Biomedical materials</subject><subject>Bone implants</subject><subject>Bone Substitutes - chemistry</subject><subject>Cell Adhesion</subject><subject>Cell Line, Transformed</subject><subject>Contact angle</subject><subject>Fibroblasts</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Ketones - chemistry</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Materials Testing</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchyme</subject><subject>Metabolism</subject><subject>Osseointegration</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Plaques</subject><subject>Polyether ether ketones</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Skin</subject><subject>Stem cells</subject><subject>Surface chemistry</subject><subject>Surface roughness</subject><subject>Surgical implants</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0EtLAzEQB_Agiu-Tdwl4EaQ1j02yOUrxBYIg3kOyO9tu3d3UJFvotzfV6sFLZhh-TIY_QheUTCkh7Hbp-qmbci5LuoeOqRBsUuiS7v_1ih-hkxiXGUsi-CE6YoqUshTlMVrcjfMehtQOc5wWgF3rbZXadZs22Dd45bsN5Hn4fj4g-QHwGLfa4sGvocO2qqCDYBPUeIAxBZtnyffYge1xgmoxtJ8jnKGDxnYRznf1FL093L_PniYvr4_Ps7uXScVFkSbSaaIbyYAXUihBWC2FzV3BAVjRqIaUpdCudrqqNVe6IdkrVgvNacNP0fXP0lXw-c-YTN_GfF5nB_BjNLRkUipChMr06h9d-jEM-TRDNVWaM8pJVjc_qgo-xgCNWYW2t2FjKDHb9E1O3zjznX7Wl7udo-uh_rO_cfMvzgGAuw</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Ajami, S</creator><creator>Coathup, M J</creator><creator>Khoury, J</creator><creator>Blunn, G W</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20170801</creationdate><title>Augmenting the bioactivity of polyetheretherketone using a novel accelerated neutral atom beam technique</title><author>Ajami, S ; Coathup, M J ; Khoury, J ; Blunn, G W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-6b909f62e34657502d65a65743ee24f7f08859bdb9cd9379f090972d5931f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adhesion tests</topic><topic>Alkaline phosphatase</topic><topic>Attachment</topic><topic>Biochemistry</topic><topic>Biocompatibility</topic><topic>Biological activity</topic><topic>Biomedical materials</topic><topic>Bone implants</topic><topic>Bone Substitutes - chemistry</topic><topic>Cell Adhesion</topic><topic>Cell Line, Transformed</topic><topic>Contact angle</topic><topic>Fibroblasts</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Ketones - chemistry</topic><topic>Materials research</topic><topic>Materials science</topic><topic>Materials Testing</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchyme</topic><topic>Metabolism</topic><topic>Osseointegration</topic><topic>Osteoblasts</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Plaques</topic><topic>Polyether ether ketones</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Skin</topic><topic>Stem cells</topic><topic>Surface chemistry</topic><topic>Surface roughness</topic><topic>Surgical implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ajami, S</creatorcontrib><creatorcontrib>Coathup, M J</creatorcontrib><creatorcontrib>Khoury, J</creatorcontrib><creatorcontrib>Blunn, G W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ajami, S</au><au>Coathup, M J</au><au>Khoury, J</au><au>Blunn, G W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Augmenting the bioactivity of polyetheretherketone using a novel accelerated neutral atom beam technique</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>105</volume><issue>6</issue><spage>1438</spage><epage>1446</epage><pages>1438-1446</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>Polyetheretherketone (PEEK) is an alternative to metallic implants in orthopedic applications; however, PEEK is bioinert and does not osteointegrate. In this study, an accelerated neutral atom beam technique (ANAB) was employed to improve the bioactivity of PEEK. The aim was to investigate the growth of human mesenchymal stem cells (hMSCs), human osteoblasts (hOB), and skin fibroblasts (BR3G) on PEEK and ANAB PEEK.
The surface roughness and contact angle of PEEK and ANAB PEEK was measured. Cell metabolic activity, proliferation and alkaline phosphatase (ALP) was measured and cell attachment was determined by quantifying adhesion plaques with cells.
ANAB treatment increased the surface hydrophilicity [91.74 ± 4.80° (PEEK) vs. 74.82 ± 2.70° (ANAB PEEK), p < 0.001] but did not alter the surface roughness. Metabolic activity and proliferation for all cell types significantly increased on ANAB PEEK compared to PEEK (p < 0.05). Significantly increased cell attachment was measured on ANAB PEEK surfaces. MSCs seeded on ANAB PEEK in the presence of osteogenic media, expressed increased levels of ALP compared to untreated PEEK (p < 0.05) CONCLUSION: Our results demonstrated that ANAB treatment increased the cell attachment, metabolic activity, and proliferation on PEEK. ANAB treatment may improve the osteointegration of PEEK implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1438-1446, 2017.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27086858</pmid><doi>10.1002/jbm.b.33681</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of biomedical materials research. Part B, Applied biomaterials, 2017-08, Vol.105 (6), p.1438-1446 |
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| subjects | Adhesion tests Alkaline phosphatase Attachment Biochemistry Biocompatibility Biological activity Biomedical materials Bone implants Bone Substitutes - chemistry Cell Adhesion Cell Line, Transformed Contact angle Fibroblasts Fibroblasts - cytology Fibroblasts - metabolism Humans Ketones - chemistry Materials research Materials science Materials Testing Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Mesenchyme Metabolism Osseointegration Osteoblasts Osteoblasts - cytology Osteoblasts - metabolism Plaques Polyether ether ketones Polyethylene Glycols - chemistry Skin Stem cells Surface chemistry Surface roughness Surgical implants |
| title | Augmenting the bioactivity of polyetheretherketone using a novel accelerated neutral atom beam technique |
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