Frog skin cultures secrete anti-yellow fever compounds
There is an urgent need to develop novel antimicrobial substances. Antimicrobial peptides (AMPs) are considered as promising candidates for future therapeutic use. Because of the re-emergence of the Flavivirus infection, and particularly the yellow fever virus (YFV), we have compared the antiviral a...
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| Veröffentlicht in: | Journal of antibiotics 2016-11, Vol.69 (11), p.783-790 |
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| creator | Muñoz-Camargo, Carolina Méndez, Margarita Correa Salazar, Vivian Moscoso, Johanna Narváez, Diana Torres, Maria Mercedes Florez, Franz Kaston Groot, Helena Mitrani, Eduardo |
| description | There is an urgent need to develop novel antimicrobial substances. Antimicrobial peptides (AMPs) are considered as promising candidates for future therapeutic use. Because of the re-emergence of the Flavivirus infection, and particularly the yellow fever virus (YFV), we have compared the antiviral activities from skin secretions of seven different frog species against YFV (strain 17D). Secretions from
Sphaenorhynchus lacteus
,
Cryptobatrachus boulongeri
and
Leptodactylus fuscus
displayed the more powerful activities.
S. lacteus
was found to inhibit viral lysis of Vero E6 cells even at the highest viral concentration evaluated of 10 LD
50
. We also report the identification of a novel frenatin-related peptide from
S. lacteus
and found that this peptide—on its own—can lead to 35% protection against YVF, while displaying no cytotoxicity against somatic cells even at fivefold higher concentrations. These results are attractive and support the need for continued exploration of new sources of AMPs from frog skin secretions such as those described here in the development of new compounds for the treatment of infectious diseases in general and specific viral infections in particular. |
| doi_str_mv | 10.1038/ja.2016.16 |
| format | Article |
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Sphaenorhynchus lacteus
,
Cryptobatrachus boulongeri
and
Leptodactylus fuscus
displayed the more powerful activities.
S. lacteus
was found to inhibit viral lysis of Vero E6 cells even at the highest viral concentration evaluated of 10 LD
50
. We also report the identification of a novel frenatin-related peptide from
S. lacteus
and found that this peptide—on its own—can lead to 35% protection against YVF, while displaying no cytotoxicity against somatic cells even at fivefold higher concentrations. These results are attractive and support the need for continued exploration of new sources of AMPs from frog skin secretions such as those described here in the development of new compounds for the treatment of infectious diseases in general and specific viral infections in particular.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.1038/ja.2016.16</identifier><identifier>PMID: 27049440</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 631/154/349 ; 631/326/22/1295 ; 631/337/2019 ; Amino Acid Sequence ; Amphibian Proteins - pharmacology ; Amphibians ; Animals ; Antiviral Agents - pharmacology ; Bacteriology ; Base Sequence ; Biomedical and Life Sciences ; Bioorganic Chemistry ; Cercopithecus aethiops ; CHO Cells ; Cloning, Molecular ; Cricetulus ; Cytotoxicity ; Infectious diseases ; Life Sciences ; Medicinal Chemistry ; Microbiology ; Organic Chemistry ; original-article ; Peptides ; Peptides - pharmacology ; Ranidae - classification ; Sequence Analysis, DNA ; Skin - chemistry ; Vector-borne diseases ; Vero Cells ; Yellow fever virus - drug effects</subject><ispartof>Journal of antibiotics, 2016-11, Vol.69 (11), p.783-790</ispartof><rights>Japan Antibiotics Research Association 2016</rights><rights>Copyright Nature Publishing Group Nov 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-67e631976abd4d89494d416569a3e2ce8ad7cf0b45517bb2cf283f960b3a343</citedby><cites>FETCH-LOGICAL-c411t-67e631976abd4d89494d416569a3e2ce8ad7cf0b45517bb2cf283f960b3a343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27049440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muñoz-Camargo, Carolina</creatorcontrib><creatorcontrib>Méndez, Margarita Correa</creatorcontrib><creatorcontrib>Salazar, Vivian</creatorcontrib><creatorcontrib>Moscoso, Johanna</creatorcontrib><creatorcontrib>Narváez, Diana</creatorcontrib><creatorcontrib>Torres, Maria Mercedes</creatorcontrib><creatorcontrib>Florez, Franz Kaston</creatorcontrib><creatorcontrib>Groot, Helena</creatorcontrib><creatorcontrib>Mitrani, Eduardo</creatorcontrib><title>Frog skin cultures secrete anti-yellow fever compounds</title><title>Journal of antibiotics</title><addtitle>J Antibiot</addtitle><addtitle>J Antibiot (Tokyo)</addtitle><description>There is an urgent need to develop novel antimicrobial substances. Antimicrobial peptides (AMPs) are considered as promising candidates for future therapeutic use. Because of the re-emergence of the Flavivirus infection, and particularly the yellow fever virus (YFV), we have compared the antiviral activities from skin secretions of seven different frog species against YFV (strain 17D). Secretions from
Sphaenorhynchus lacteus
,
Cryptobatrachus boulongeri
and
Leptodactylus fuscus
displayed the more powerful activities.
S. lacteus
was found to inhibit viral lysis of Vero E6 cells even at the highest viral concentration evaluated of 10 LD
50
. We also report the identification of a novel frenatin-related peptide from
S. lacteus
and found that this peptide—on its own—can lead to 35% protection against YVF, while displaying no cytotoxicity against somatic cells even at fivefold higher concentrations. These results are attractive and support the need for continued exploration of new sources of AMPs from frog skin secretions such as those described here in the development of new compounds for the treatment of infectious diseases in general and specific viral infections in particular.</description><subject>13</subject><subject>631/154/349</subject><subject>631/326/22/1295</subject><subject>631/337/2019</subject><subject>Amino Acid Sequence</subject><subject>Amphibian Proteins - pharmacology</subject><subject>Amphibians</subject><subject>Animals</subject><subject>Antiviral Agents - pharmacology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Bioorganic Chemistry</subject><subject>Cercopithecus aethiops</subject><subject>CHO Cells</subject><subject>Cloning, Molecular</subject><subject>Cricetulus</subject><subject>Cytotoxicity</subject><subject>Infectious diseases</subject><subject>Life Sciences</subject><subject>Medicinal Chemistry</subject><subject>Microbiology</subject><subject>Organic Chemistry</subject><subject>original-article</subject><subject>Peptides</subject><subject>Peptides - pharmacology</subject><subject>Ranidae - classification</subject><subject>Sequence Analysis, DNA</subject><subject>Skin - chemistry</subject><subject>Vector-borne diseases</subject><subject>Vero Cells</subject><subject>Yellow fever virus - drug effects</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpl0M9LwzAUB_AgipvTi3-AFLyI0plfS9KjDKfCwIPeQ5q-jta2mUmr7L83Y1NET-_wPnzf44vQOcFTgpm6rc2UYiKmRBygMVGKpISL7BCNMaYkVYriEToJocaYSSbVMRpRiXnGOR4jsfBulYS3qkvs0PSDh5AEsB56SEzXV-kGmsZ9JiV8gE-sa9du6Ipwio5K0wQ4288Jelncv84f0-Xzw9P8bplaTkifCgmCkUwKkxe8UFk8WnAiZiIzDKgFZQppS5zz2YzIPKe2pIqVmcA5M4yzCbrapa69ex8g9Lqtgo0PmQ7cEDRRVAjBMZWRXv6htRt8F3-LijM149FGdb1T1rsQPJR67avW-I0mWG-71LXR2y412eKLfeSQt1D80O_yIrjZgRBX3Qr8r5v_474A6xl7Xg</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Muñoz-Camargo, Carolina</creator><creator>Méndez, Margarita Correa</creator><creator>Salazar, Vivian</creator><creator>Moscoso, Johanna</creator><creator>Narváez, Diana</creator><creator>Torres, Maria Mercedes</creator><creator>Florez, Franz Kaston</creator><creator>Groot, Helena</creator><creator>Mitrani, Eduardo</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20161101</creationdate><title>Frog skin cultures secrete anti-yellow fever compounds</title><author>Muñoz-Camargo, Carolina ; Méndez, Margarita Correa ; Salazar, Vivian ; Moscoso, Johanna ; Narváez, Diana ; Torres, Maria Mercedes ; Florez, Franz Kaston ; Groot, Helena ; Mitrani, Eduardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-67e631976abd4d89494d416569a3e2ce8ad7cf0b45517bb2cf283f960b3a343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>13</topic><topic>631/154/349</topic><topic>631/326/22/1295</topic><topic>631/337/2019</topic><topic>Amino Acid Sequence</topic><topic>Amphibian Proteins - 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Academic</collection><jtitle>Journal of antibiotics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muñoz-Camargo, Carolina</au><au>Méndez, Margarita Correa</au><au>Salazar, Vivian</au><au>Moscoso, Johanna</au><au>Narváez, Diana</au><au>Torres, Maria Mercedes</au><au>Florez, Franz Kaston</au><au>Groot, Helena</au><au>Mitrani, Eduardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Frog skin cultures secrete anti-yellow fever compounds</atitle><jtitle>Journal of antibiotics</jtitle><stitle>J Antibiot</stitle><addtitle>J Antibiot (Tokyo)</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>69</volume><issue>11</issue><spage>783</spage><epage>790</epage><pages>783-790</pages><issn>0021-8820</issn><eissn>1881-1469</eissn><abstract>There is an urgent need to develop novel antimicrobial substances. Antimicrobial peptides (AMPs) are considered as promising candidates for future therapeutic use. Because of the re-emergence of the Flavivirus infection, and particularly the yellow fever virus (YFV), we have compared the antiviral activities from skin secretions of seven different frog species against YFV (strain 17D). Secretions from
Sphaenorhynchus lacteus
,
Cryptobatrachus boulongeri
and
Leptodactylus fuscus
displayed the more powerful activities.
S. lacteus
was found to inhibit viral lysis of Vero E6 cells even at the highest viral concentration evaluated of 10 LD
50
. We also report the identification of a novel frenatin-related peptide from
S. lacteus
and found that this peptide—on its own—can lead to 35% protection against YVF, while displaying no cytotoxicity against somatic cells even at fivefold higher concentrations. These results are attractive and support the need for continued exploration of new sources of AMPs from frog skin secretions such as those described here in the development of new compounds for the treatment of infectious diseases in general and specific viral infections in particular.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27049440</pmid><doi>10.1038/ja.2016.16</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | 13 631/154/349 631/326/22/1295 631/337/2019 Amino Acid Sequence Amphibian Proteins - pharmacology Amphibians Animals Antiviral Agents - pharmacology Bacteriology Base Sequence Biomedical and Life Sciences Bioorganic Chemistry Cercopithecus aethiops CHO Cells Cloning, Molecular Cricetulus Cytotoxicity Infectious diseases Life Sciences Medicinal Chemistry Microbiology Organic Chemistry original-article Peptides Peptides - pharmacology Ranidae - classification Sequence Analysis, DNA Skin - chemistry Vector-borne diseases Vero Cells Yellow fever virus - drug effects |
| title | Frog skin cultures secrete anti-yellow fever compounds |
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