Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi
Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the mod...
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| Veröffentlicht in: | Chemical science (Cambridge) 2016-03, Vol.7 (3), p.1797-1806 |
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| creator | Bagert, John D van Kessel, Julia C Sweredoski, Michael J Feng, Lihui Hess, Sonja Bassler, Bonnie L Tirrell, David A |
| description | Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism
by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of
from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis. |
| doi_str_mv | 10.1039/c5sc03340c |
| format | Article |
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by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of
from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.</description><identifier>ISSN: 2041-6520</identifier><identifier>EISSN: 2041-6539</identifier><identifier>DOI: 10.1039/c5sc03340c</identifier><identifier>PMID: 26925210</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Amino acids ; Bacteria ; Chemistry ; Communities ; Detection ; Mathematical models ; Population density ; Proteins ; Vibrio ; Vibrio harveyi</subject><ispartof>Chemical science (Cambridge), 2016-03, Vol.7 (3), p.1797-1806</ispartof><rights>This journal is © The Royal Society of Chemistry 2015 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-c56ebac4aedd3464c4b84487e17e8b523b9687e5e5271ff3d2c77ed177a381263</citedby><cites>FETCH-LOGICAL-c444t-c56ebac4aedd3464c4b84487e17e8b523b9687e5e5271ff3d2c77ed177a381263</cites><orcidid>0000-0001-7768-2853</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763989/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763989/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26925210$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bagert, John D</creatorcontrib><creatorcontrib>van Kessel, Julia C</creatorcontrib><creatorcontrib>Sweredoski, Michael J</creatorcontrib><creatorcontrib>Feng, Lihui</creatorcontrib><creatorcontrib>Hess, Sonja</creatorcontrib><creatorcontrib>Bassler, Bonnie L</creatorcontrib><creatorcontrib>Tirrell, David A</creatorcontrib><title>Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi</title><title>Chemical science (Cambridge)</title><addtitle>Chem Sci</addtitle><description>Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism
by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of
from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.</description><subject>Amino acids</subject><subject>Bacteria</subject><subject>Chemistry</subject><subject>Communities</subject><subject>Detection</subject><subject>Mathematical models</subject><subject>Population density</subject><subject>Proteins</subject><subject>Vibrio</subject><subject>Vibrio harveyi</subject><issn>2041-6520</issn><issn>2041-6539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkU1Lw0AQhhdRbNFe_AGSowjR_d7kIkj8hIIHP67LZjPRlSTb7jaF_nujrUVPOpeZYR5eZuZF6IjgM4JZfm5FtJgxju0OGlPMSSoFy3e3NcUjNInxHQ_BGBFU7aMRlTkVlOAxunpyLaQBom-WUCWz4BfgW2cT05lmFV1MfJ3Mex_6NonQRde9Jq5LXlwZnE_eTFjCyh2ivdo0ESabfICeb66firt0-nB7X1xOU8s5X6RWSCiN5QaqinHJLS8zzjMFREFWCsrKXA6dgGFJUtesolYpqIhShmWESnaALta6s75sobLQLYJp9Cy41oSV9sbp35POvelXv9RcSZZn-SBwshEIft5DXOjWRQtNYzrwfdQko1IKRbn6G1U5o8N7Cf8HqqSUnORiQE_XqA0-xgD1dnmC9aefuhCPxZefxQAf_zx3i367xz4Aunea9g</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Bagert, John D</creator><creator>van Kessel, Julia C</creator><creator>Sweredoski, Michael J</creator><creator>Feng, Lihui</creator><creator>Hess, Sonja</creator><creator>Bassler, Bonnie L</creator><creator>Tirrell, David A</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7768-2853</orcidid></search><sort><creationdate>20160301</creationdate><title>Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi</title><author>Bagert, John D ; van Kessel, Julia C ; Sweredoski, Michael J ; Feng, Lihui ; Hess, Sonja ; Bassler, Bonnie L ; Tirrell, David A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-c56ebac4aedd3464c4b84487e17e8b523b9687e5e5271ff3d2c77ed177a381263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino acids</topic><topic>Bacteria</topic><topic>Chemistry</topic><topic>Communities</topic><topic>Detection</topic><topic>Mathematical models</topic><topic>Population density</topic><topic>Proteins</topic><topic>Vibrio</topic><topic>Vibrio harveyi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bagert, John D</creatorcontrib><creatorcontrib>van Kessel, Julia C</creatorcontrib><creatorcontrib>Sweredoski, Michael J</creatorcontrib><creatorcontrib>Feng, Lihui</creatorcontrib><creatorcontrib>Hess, Sonja</creatorcontrib><creatorcontrib>Bassler, Bonnie L</creatorcontrib><creatorcontrib>Tirrell, David A</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chemical science (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bagert, John D</au><au>van Kessel, Julia C</au><au>Sweredoski, Michael J</au><au>Feng, Lihui</au><au>Hess, Sonja</au><au>Bassler, Bonnie L</au><au>Tirrell, David A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi</atitle><jtitle>Chemical science (Cambridge)</jtitle><addtitle>Chem Sci</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>7</volume><issue>3</issue><spage>1797</spage><epage>1806</epage><pages>1797-1806</pages><issn>2041-6520</issn><eissn>2041-6539</eissn><abstract>Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism
by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of
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| subjects | Amino acids Bacteria Chemistry Communities Detection Mathematical models Population density Proteins Vibrio Vibrio harveyi |
| title | Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi |
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