Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver
The liver is an essential metabolically active organ, responsible for many vital life functions such as metabolism, synthesis, and storage. This study describes the macroscopic and microscopic zebrafish liver and its temporal changes during a period of 13 weeks. Forty-six adult zebrafish were used (...
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Veröffentlicht in: | Folia morphologica 2016, Vol.75 (3), p.382-387 |
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description | The liver is an essential metabolically active organ, responsible for many vital life functions such as metabolism, synthesis, and storage. This study describes the macroscopic and microscopic zebrafish liver and its temporal changes during a period of 13 weeks. Forty-six adult zebrafish were used (46), of which 27 were females and were 19 males. Seven fish were euthanized initially for macroscopic evaluation and 3 fish were euthanized weekly for 13 weeks for microscopic evaluation. Routine histological technique was performed; the slides were stained with haematoxylin/eosin and periodic acid-Schiff. The volumetric (Vv(h)) and numerical (Nv(h)) densities of hepatocytes and vacuolation intensity were determined weekly. The liver was located in the coelomic cavity ventrally to the swim bladder, slightly brownish and divided into 3 hepatic lobes. Microscopically, it consisted of hepatocytes forming a muralium duplex around the sinusoids. The staining of the cytoplasm of hepatocytes was more eosinophilic and more basophilic in males and females, respectively. There was no formation of portal triads and phagocytic cells. The gallbladder had transitional epithelium, and the bile ducts had simple cuboidal epithelium. The volume and numerical density of hepatocytes during the 13 weeks was not statistically different, but these parameters displayed a positive correlation. The vacuolation was due to the presence of glycogen. The zebrafish liver has macro and micro structural particular features while, quantitatively, the volume and number of hepatocytes showed great capacity to respond to the physiological needs of the animal at the time. |
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This study describes the macroscopic and microscopic zebrafish liver and its temporal changes during a period of 13 weeks. Forty-six adult zebrafish were used (46), of which 27 were females and were 19 males. Seven fish were euthanized initially for macroscopic evaluation and 3 fish were euthanized weekly for 13 weeks for microscopic evaluation. Routine histological technique was performed; the slides were stained with haematoxylin/eosin and periodic acid-Schiff. The volumetric (Vv(h)) and numerical (Nv(h)) densities of hepatocytes and vacuolation intensity were determined weekly. The liver was located in the coelomic cavity ventrally to the swim bladder, slightly brownish and divided into 3 hepatic lobes. Microscopically, it consisted of hepatocytes forming a muralium duplex around the sinusoids. The staining of the cytoplasm of hepatocytes was more eosinophilic and more basophilic in males and females, respectively. There was no formation of portal triads and phagocytic cells. The gallbladder had transitional epithelium, and the bile ducts had simple cuboidal epithelium. The volume and numerical density of hepatocytes during the 13 weeks was not statistically different, but these parameters displayed a positive correlation. The vacuolation was due to the presence of glycogen. The zebrafish liver has macro and micro structural particular features while, quantitatively, the volume and number of hepatocytes showed great capacity to respond to the physiological needs of the animal at the time.</description><identifier>ISSN: 0015-5659</identifier><identifier>EISSN: 1644-3284</identifier><identifier>DOI: 10.5603/FM.a2016.0009</identifier><identifier>PMID: 26916203</identifier><language>eng</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Animals ; Bile ducts ; Cytoplasm ; Danio rerio ; Epithelium ; Female ; Gallbladder ; Glycogen ; Hepatocytes ; Leukocytes (eosinophilic) ; Liver ; Male ; Phagocytes ; Staining and Labeling ; Swim bladder ; Zebrafish</subject><ispartof>Folia morphologica, 2016, Vol.75 (3), p.382-387</ispartof><rights>2016. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-a3a1f42203bb794e352509c78c4c7feea93ce4b8d56de2c8ba776aeeb9d22ba53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26916203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Oliveira, L B</creatorcontrib><creatorcontrib>Leonardo, A S</creatorcontrib><creatorcontrib>de Lima, E M M</creatorcontrib><title>Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver</title><title>Folia morphologica</title><addtitle>Folia Morphol (Warsz)</addtitle><description>The liver is an essential metabolically active organ, responsible for many vital life functions such as metabolism, synthesis, and storage. This study describes the macroscopic and microscopic zebrafish liver and its temporal changes during a period of 13 weeks. Forty-six adult zebrafish were used (46), of which 27 were females and were 19 males. Seven fish were euthanized initially for macroscopic evaluation and 3 fish were euthanized weekly for 13 weeks for microscopic evaluation. Routine histological technique was performed; the slides were stained with haematoxylin/eosin and periodic acid-Schiff. The volumetric (Vv(h)) and numerical (Nv(h)) densities of hepatocytes and vacuolation intensity were determined weekly. The liver was located in the coelomic cavity ventrally to the swim bladder, slightly brownish and divided into 3 hepatic lobes. Microscopically, it consisted of hepatocytes forming a muralium duplex around the sinusoids. The staining of the cytoplasm of hepatocytes was more eosinophilic and more basophilic in males and females, respectively. There was no formation of portal triads and phagocytic cells. The gallbladder had transitional epithelium, and the bile ducts had simple cuboidal epithelium. The volume and numerical density of hepatocytes during the 13 weeks was not statistically different, but these parameters displayed a positive correlation. The vacuolation was due to the presence of glycogen. The zebrafish liver has macro and micro structural particular features while, quantitatively, the volume and number of hepatocytes showed great capacity to respond to the physiological needs of the animal at the time.</description><subject>Animals</subject><subject>Bile ducts</subject><subject>Cytoplasm</subject><subject>Danio rerio</subject><subject>Epithelium</subject><subject>Female</subject><subject>Gallbladder</subject><subject>Glycogen</subject><subject>Hepatocytes</subject><subject>Leukocytes (eosinophilic)</subject><subject>Liver</subject><subject>Male</subject><subject>Phagocytes</subject><subject>Staining and Labeling</subject><subject>Swim bladder</subject><subject>Zebrafish</subject><issn>0015-5659</issn><issn>1644-3284</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkD1PwzAURS0EoqUwsiJLLGVIefFXkhEVCkitWGC2HOdFdZUmxU6Q4NeT0MLA9O5wdO_TIeQyhplUwG8Xq5lhEKsZAGRHZBwrISLOUnFMxgCxjKSS2YichbABkEIkcEpGTGWxYsDHZLky1jcRNXVBt66PofWdbTtvKlpgsN7tWtfUgTYlbddIvzD3pnRhTaf3pnYN9ehdc0Mr94H-nJyUpgp4cbgT8rZ4eJ0_RcuXx-f53TKyXEEbGW7iUrB-P8-TTCCXTEJmk9QKm5SIJuMWRZ4WUhXIbJqbJFEGMc8KxnIj-YRM970737x3GFq9dcFiVZkamy7oOGVKSQFsQK__oZum83X_nWZCCQZpmg1UtKcGAcFjqXfebY3_1DHoQbNerPSPZj1o7vmrQ2uXb7H4o3-98m_OoXeC</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>de Oliveira, L B</creator><creator>Leonardo, A S</creator><creator>de Lima, E M M</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H9R</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>2016</creationdate><title>Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver</title><author>de Oliveira, L B ; Leonardo, A S ; de Lima, E M M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-a3a1f42203bb794e352509c78c4c7feea93ce4b8d56de2c8ba776aeeb9d22ba53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Bile ducts</topic><topic>Cytoplasm</topic><topic>Danio rerio</topic><topic>Epithelium</topic><topic>Female</topic><topic>Gallbladder</topic><topic>Glycogen</topic><topic>Hepatocytes</topic><topic>Leukocytes (eosinophilic)</topic><topic>Liver</topic><topic>Male</topic><topic>Phagocytes</topic><topic>Staining and Labeling</topic><topic>Swim bladder</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira, L B</creatorcontrib><creatorcontrib>Leonardo, A S</creatorcontrib><creatorcontrib>de Lima, E M M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest - Health & Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Illustrata: Natural Sciences</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Folia morphologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Oliveira, L B</au><au>Leonardo, A S</au><au>de Lima, E M M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver</atitle><jtitle>Folia morphologica</jtitle><addtitle>Folia Morphol (Warsz)</addtitle><date>2016</date><risdate>2016</risdate><volume>75</volume><issue>3</issue><spage>382</spage><epage>387</epage><pages>382-387</pages><issn>0015-5659</issn><eissn>1644-3284</eissn><abstract>The liver is an essential metabolically active organ, responsible for many vital life functions such as metabolism, synthesis, and storage. This study describes the macroscopic and microscopic zebrafish liver and its temporal changes during a period of 13 weeks. Forty-six adult zebrafish were used (46), of which 27 were females and were 19 males. Seven fish were euthanized initially for macroscopic evaluation and 3 fish were euthanized weekly for 13 weeks for microscopic evaluation. Routine histological technique was performed; the slides were stained with haematoxylin/eosin and periodic acid-Schiff. The volumetric (Vv(h)) and numerical (Nv(h)) densities of hepatocytes and vacuolation intensity were determined weekly. The liver was located in the coelomic cavity ventrally to the swim bladder, slightly brownish and divided into 3 hepatic lobes. Microscopically, it consisted of hepatocytes forming a muralium duplex around the sinusoids. The staining of the cytoplasm of hepatocytes was more eosinophilic and more basophilic in males and females, respectively. There was no formation of portal triads and phagocytic cells. 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subjects | Animals Bile ducts Cytoplasm Danio rerio Epithelium Female Gallbladder Glycogen Hepatocytes Leukocytes (eosinophilic) Liver Male Phagocytes Staining and Labeling Swim bladder Zebrafish |
title | Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver |
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