Induction of cell self-organization on weakly positively charged surfaces prepared by the deposition of polyion complex nanoparticles of thermoresponsive, zwitterionic copolymers
We have developed inducible cell self-organization through weakly positively charged culture surfaces. In this study, a thermoresponsive and zwitterionic copolymer comprised of N,N-dimethylaminoethyl methacrylate (DMAEMA) and methacrylic acid (MA) (PDMAEMA-co-PMA; Mn: ∼9.7 × 10 g/mol; PDMAEMA/PMA ra...
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| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2017-07, Vol.105 (5), p.1009-1015 |
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| container_title | Journal of biomedical materials research. Part B, Applied biomaterials |
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| creator | Iwai, Ryosuke Haruki, Ryota Nemoto, Yasushi Nakayama, Yasuhide |
| description | We have developed inducible cell self-organization through weakly positively charged culture surfaces. In this study, a thermoresponsive and zwitterionic copolymer comprised of N,N-dimethylaminoethyl methacrylate (DMAEMA) and methacrylic acid (MA) (PDMAEMA-co-PMA; Mn: ∼9.7 × 10
g/mol; PDMAEMA/PMA ratio: 10) was designed for inducing cell self-organization. The copolymer formed single polymer-derived polyion complex (sPIC) nanoparticles following dissolution in an aqueous solution. The sPIC nanoparticles had a positive charge (ca. 25 mV). Self-organization occurred in adipose-derived vascular stromal cell monolayers cultivated on sPIC-deposited surfaces. There were dramatic morphological changes of these cells with the formation of capillary-like networks and single-cell aggregates with little cytotoxicity. This was a significant improvement compared with cells grown on previously developed surfaces deposited with PIC, a mixture of PDMAEMA and plasmid DNA. Thus, sPICs of PDMAEMA-co-PMA may allow for the accurate evaluation of a variety of cell behaviors with less cytotoxicity, and may facilitate additional potential medical applications such as cell-based therapy and drug discovery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1009-1015, 2017. |
| doi_str_mv | 10.1002/jbm.b.33638 |
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g/mol; PDMAEMA/PMA ratio: 10) was designed for inducing cell self-organization. The copolymer formed single polymer-derived polyion complex (sPIC) nanoparticles following dissolution in an aqueous solution. The sPIC nanoparticles had a positive charge (ca. 25 mV). Self-organization occurred in adipose-derived vascular stromal cell monolayers cultivated on sPIC-deposited surfaces. There were dramatic morphological changes of these cells with the formation of capillary-like networks and single-cell aggregates with little cytotoxicity. This was a significant improvement compared with cells grown on previously developed surfaces deposited with PIC, a mixture of PDMAEMA and plasmid DNA. Thus, sPICs of PDMAEMA-co-PMA may allow for the accurate evaluation of a variety of cell behaviors with less cytotoxicity, and may facilitate additional potential medical applications such as cell-based therapy and drug discovery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1009-1015, 2017.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.33638</identifier><identifier>PMID: 26892839</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adipose Tissue - cytology ; Adipose Tissue - metabolism ; Aggregates ; Animals ; Biocompatibility ; Biomedical materials ; Cell culture ; Copolymers ; Cytotoxicity ; Deoxyribonucleic acid ; Deposition ; Dissolution ; DNA ; Drug discovery ; Evaluation ; Materials research ; Materials science ; Materials Testing ; Methacrylic acid ; Monolayers ; Nanoparticles ; Nanoparticles - chemistry ; Networks ; Plasmids ; Polymethacrylic Acids - chemical synthesis ; Polymethacrylic Acids - chemistry ; Rats ; Stem Cells - cytology ; Stem Cells - metabolism ; Therapy ; Toxicity ; Zwitterions</subject><ispartof>Journal of biomedical materials research. Part B, Applied biomaterials, 2017-07, Vol.105 (5), p.1009-1015</ispartof><rights>2016 Wiley Periodicals, Inc.</rights><rights>2017 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-1e86c7fa528f1c8b1d7d594fa7b47b5b4f1e83181675fa6f20bf315eb05d99453</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26892839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iwai, Ryosuke</creatorcontrib><creatorcontrib>Haruki, Ryota</creatorcontrib><creatorcontrib>Nemoto, Yasushi</creatorcontrib><creatorcontrib>Nakayama, Yasuhide</creatorcontrib><title>Induction of cell self-organization on weakly positively charged surfaces prepared by the deposition of polyion complex nanoparticles of thermoresponsive, zwitterionic copolymers</title><title>Journal of biomedical materials research. Part B, Applied biomaterials</title><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><description>We have developed inducible cell self-organization through weakly positively charged culture surfaces. In this study, a thermoresponsive and zwitterionic copolymer comprised of N,N-dimethylaminoethyl methacrylate (DMAEMA) and methacrylic acid (MA) (PDMAEMA-co-PMA; Mn: ∼9.7 × 10
g/mol; PDMAEMA/PMA ratio: 10) was designed for inducing cell self-organization. The copolymer formed single polymer-derived polyion complex (sPIC) nanoparticles following dissolution in an aqueous solution. The sPIC nanoparticles had a positive charge (ca. 25 mV). Self-organization occurred in adipose-derived vascular stromal cell monolayers cultivated on sPIC-deposited surfaces. There were dramatic morphological changes of these cells with the formation of capillary-like networks and single-cell aggregates with little cytotoxicity. This was a significant improvement compared with cells grown on previously developed surfaces deposited with PIC, a mixture of PDMAEMA and plasmid DNA. Thus, sPICs of PDMAEMA-co-PMA may allow for the accurate evaluation of a variety of cell behaviors with less cytotoxicity, and may facilitate additional potential medical applications such as cell-based therapy and drug discovery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1009-1015, 2017.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - metabolism</subject><subject>Aggregates</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Cell culture</subject><subject>Copolymers</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>Deposition</subject><subject>Dissolution</subject><subject>DNA</subject><subject>Drug discovery</subject><subject>Evaluation</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Materials Testing</subject><subject>Methacrylic acid</subject><subject>Monolayers</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Networks</subject><subject>Plasmids</subject><subject>Polymethacrylic Acids - chemical synthesis</subject><subject>Polymethacrylic Acids - chemistry</subject><subject>Rats</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Therapy</subject><subject>Toxicity</subject><subject>Zwitterions</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUFP3DAQha0KVLbQU-_IEhcOzRLbseMcESrtSitxgXNkO2PwktjBTrpdfha_EK-W9tDTPI2_98aaQegbKZekLOnVRg9LvWRMMPkJLQjntKgaSY7-6ZqdoC8pbTIsSs4-oxMqZEMlaxbobeW72UwueBwsNtD3OEFvixAflXev6vDi8RbUc7_DY0hucr8hS_Ok4iN0OM3RKgMJjxFGFXNH7_D0BLiDA32IHkO_20sThrGHP9grHzI-OdNnbwayJQ4hQhqDT3nEd_y6ddMEMbucyb59wgAxnaFjq_oEXz_qKXq4_XF_86tY3_1c3VyvC8OqeioISGFqqziVlhipSVd3vKmsqnVVa64rmwlGJBE1t0pYWmrLCAdd8q5pKs5O0eUhd4zhZYY0tYNL-w0pD2FOLZFUCE4ryjJ68R-6CXP0-XctaUpZS9pUIlPnH9SsB-jaMbpBxV379xrsHTsOkWg</recordid><startdate>20170701</startdate><enddate>20170701</enddate><creator>Iwai, Ryosuke</creator><creator>Haruki, Ryota</creator><creator>Nemoto, Yasushi</creator><creator>Nakayama, Yasuhide</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20170701</creationdate><title>Induction of cell self-organization on weakly positively charged surfaces prepared by the deposition of polyion complex nanoparticles of thermoresponsive, zwitterionic copolymers</title><author>Iwai, Ryosuke ; Haruki, Ryota ; Nemoto, Yasushi ; Nakayama, Yasuhide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-1e86c7fa528f1c8b1d7d594fa7b47b5b4f1e83181675fa6f20bf315eb05d99453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - metabolism</topic><topic>Aggregates</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Cell culture</topic><topic>Copolymers</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>Deposition</topic><topic>Dissolution</topic><topic>DNA</topic><topic>Drug discovery</topic><topic>Evaluation</topic><topic>Materials research</topic><topic>Materials science</topic><topic>Materials Testing</topic><topic>Methacrylic acid</topic><topic>Monolayers</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Networks</topic><topic>Plasmids</topic><topic>Polymethacrylic Acids - chemical synthesis</topic><topic>Polymethacrylic Acids - chemistry</topic><topic>Rats</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Therapy</topic><topic>Toxicity</topic><topic>Zwitterions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iwai, Ryosuke</creatorcontrib><creatorcontrib>Haruki, Ryota</creatorcontrib><creatorcontrib>Nemoto, Yasushi</creatorcontrib><creatorcontrib>Nakayama, Yasuhide</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iwai, Ryosuke</au><au>Haruki, Ryota</au><au>Nemoto, Yasushi</au><au>Nakayama, Yasuhide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of cell self-organization on weakly positively charged surfaces prepared by the deposition of polyion complex nanoparticles of thermoresponsive, zwitterionic copolymers</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><date>2017-07-01</date><risdate>2017</risdate><volume>105</volume><issue>5</issue><spage>1009</spage><epage>1015</epage><pages>1009-1015</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>We have developed inducible cell self-organization through weakly positively charged culture surfaces. In this study, a thermoresponsive and zwitterionic copolymer comprised of N,N-dimethylaminoethyl methacrylate (DMAEMA) and methacrylic acid (MA) (PDMAEMA-co-PMA; Mn: ∼9.7 × 10
g/mol; PDMAEMA/PMA ratio: 10) was designed for inducing cell self-organization. The copolymer formed single polymer-derived polyion complex (sPIC) nanoparticles following dissolution in an aqueous solution. The sPIC nanoparticles had a positive charge (ca. 25 mV). Self-organization occurred in adipose-derived vascular stromal cell monolayers cultivated on sPIC-deposited surfaces. There were dramatic morphological changes of these cells with the formation of capillary-like networks and single-cell aggregates with little cytotoxicity. This was a significant improvement compared with cells grown on previously developed surfaces deposited with PIC, a mixture of PDMAEMA and plasmid DNA. Thus, sPICs of PDMAEMA-co-PMA may allow for the accurate evaluation of a variety of cell behaviors with less cytotoxicity, and may facilitate additional potential medical applications such as cell-based therapy and drug discovery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1009-1015, 2017.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>26892839</pmid><doi>10.1002/jbm.b.33638</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of biomedical materials research. Part B, Applied biomaterials, 2017-07, Vol.105 (5), p.1009-1015 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adipose Tissue - cytology Adipose Tissue - metabolism Aggregates Animals Biocompatibility Biomedical materials Cell culture Copolymers Cytotoxicity Deoxyribonucleic acid Deposition Dissolution DNA Drug discovery Evaluation Materials research Materials science Materials Testing Methacrylic acid Monolayers Nanoparticles Nanoparticles - chemistry Networks Plasmids Polymethacrylic Acids - chemical synthesis Polymethacrylic Acids - chemistry Rats Stem Cells - cytology Stem Cells - metabolism Therapy Toxicity Zwitterions |
| title | Induction of cell self-organization on weakly positively charged surfaces prepared by the deposition of polyion complex nanoparticles of thermoresponsive, zwitterionic copolymers |
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