Sunitinib modulates the radiosensitivity of esophageal squamous cell carcinoma cells in vitro
SUMMARY This study aims to explore the radiosensitivity of sunitinib on esophageal cancer cell lines. For in vitro studies, human esophageal squamous cell carcinoma (ESCC) cell lines were treated with sunitinib 24 hours before irradiation. ESCC cell lines were treated with sunitinib with or without...
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| Veröffentlicht in: | Diseases of the esophagus 2016-11, Vol.30 (7), p.1-8 |
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| creator | Ding, Y.-Q. Zhu, H.-C. Chen, X.-C. Sun, X.-C. Yang, X. Qin, Q. Zhang, H. Yang, Y. Yang, Y.-H. Gao, L. Luo, J.-D. Zhou, X.-F. |
| description | SUMMARY
This study aims to explore the radiosensitivity of sunitinib on esophageal cancer cell lines. For in vitro studies, human esophageal squamous cell carcinoma (ESCC) cell lines were treated with sunitinib 24 hours before irradiation. ESCC cell lines were treated with sunitinib with or without radiation. Cell proliferation was detected by Cell Counting Kit 8 assay. Radiosensitization was evaluated by clonogenic survival assay. Cell apoptosis and cell cycle analysis were detected by flow cytometry. Deoxyribonucleic acid (DNA) double-strand breaks were performed by immunocytofluorescence analysis. Western blot analysis was used to determine the effect of sunitinib on radiation induced signal transduction. Sunitinib potently sensitized ESCC cells to radiation with a sensitization enhancement ratio of 1.13–1.72. Furthermore, sunitinib increased radiation induced DNA double-strand breaks, promoted the apoptosis of ESCC cells and induced the G2/M arrest. Radiosensitization was accompanied with enhanced apoptosis and regulated by the intrinsic pathway of apoptosis. Sunitinib sensitized ESCC cells to the cytotoxic effects of radiation. This compound is promising for future clinical trials with chemoradiation in esophageal cancer. |
| doi_str_mv | 10.1111/dote.12440 |
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This study aims to explore the radiosensitivity of sunitinib on esophageal cancer cell lines. For in vitro studies, human esophageal squamous cell carcinoma (ESCC) cell lines were treated with sunitinib 24 hours before irradiation. ESCC cell lines were treated with sunitinib with or without radiation. Cell proliferation was detected by Cell Counting Kit 8 assay. Radiosensitization was evaluated by clonogenic survival assay. Cell apoptosis and cell cycle analysis were detected by flow cytometry. Deoxyribonucleic acid (DNA) double-strand breaks were performed by immunocytofluorescence analysis. Western blot analysis was used to determine the effect of sunitinib on radiation induced signal transduction. Sunitinib potently sensitized ESCC cells to radiation with a sensitization enhancement ratio of 1.13–1.72. Furthermore, sunitinib increased radiation induced DNA double-strand breaks, promoted the apoptosis of ESCC cells and induced the G2/M arrest. Radiosensitization was accompanied with enhanced apoptosis and regulated by the intrinsic pathway of apoptosis. Sunitinib sensitized ESCC cells to the cytotoxic effects of radiation. This compound is promising for future clinical trials with chemoradiation in esophageal cancer.</description><identifier>ISSN: 1120-8694</identifier><identifier>EISSN: 1442-2050</identifier><identifier>DOI: 10.1111/dote.12440</identifier><identifier>PMID: 26542732</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Antineoplastic Agents - pharmacology ; apoptosis ; Apoptosis - drug effects ; Blotting, Western ; Carcinoma, Squamous Cell - therapy ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Chemoradiotherapy ; DNA Breaks, Double-Stranded ; esophageal cancer ; Esophageal Neoplasms - therapy ; Esophageal Squamous Cell Carcinoma ; Flow Cytometry ; Humans ; Indoles - pharmacology ; Pyrroles - pharmacology ; Radiation Tolerance - drug effects ; Radiation-Sensitizing Agents - pharmacology ; radiosensitization ; Sunitinib</subject><ispartof>Diseases of the esophagus, 2016-11, Vol.30 (7), p.1-8</ispartof><rights>2017 International Society for Diseases of the Esophagus 2017</rights><rights>2015 International Society for Diseases of the Esophagus</rights><rights>2015 International Society for Diseases of the Esophagus.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3240-f2dd55153589fea9636b5c8fef48245b71757f22e884eb2a058b21288ce8e7f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fdote.12440$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fdote.12440$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26542732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ding, Y.-Q.</creatorcontrib><creatorcontrib>Zhu, H.-C.</creatorcontrib><creatorcontrib>Chen, X.-C.</creatorcontrib><creatorcontrib>Sun, X.-C.</creatorcontrib><creatorcontrib>Yang, X.</creatorcontrib><creatorcontrib>Qin, Q.</creatorcontrib><creatorcontrib>Zhang, H.</creatorcontrib><creatorcontrib>Yang, Y.</creatorcontrib><creatorcontrib>Yang, Y.-H.</creatorcontrib><creatorcontrib>Gao, L.</creatorcontrib><creatorcontrib>Luo, J.-D.</creatorcontrib><creatorcontrib>Zhou, X.-F.</creatorcontrib><title>Sunitinib modulates the radiosensitivity of esophageal squamous cell carcinoma cells in vitro</title><title>Diseases of the esophagus</title><addtitle>Dis Esophagus</addtitle><description>SUMMARY
This study aims to explore the radiosensitivity of sunitinib on esophageal cancer cell lines. For in vitro studies, human esophageal squamous cell carcinoma (ESCC) cell lines were treated with sunitinib 24 hours before irradiation. ESCC cell lines were treated with sunitinib with or without radiation. Cell proliferation was detected by Cell Counting Kit 8 assay. Radiosensitization was evaluated by clonogenic survival assay. Cell apoptosis and cell cycle analysis were detected by flow cytometry. Deoxyribonucleic acid (DNA) double-strand breaks were performed by immunocytofluorescence analysis. Western blot analysis was used to determine the effect of sunitinib on radiation induced signal transduction. Sunitinib potently sensitized ESCC cells to radiation with a sensitization enhancement ratio of 1.13–1.72. Furthermore, sunitinib increased radiation induced DNA double-strand breaks, promoted the apoptosis of ESCC cells and induced the G2/M arrest. Radiosensitization was accompanied with enhanced apoptosis and regulated by the intrinsic pathway of apoptosis. Sunitinib sensitized ESCC cells to the cytotoxic effects of radiation. This compound is promising for future clinical trials with chemoradiation in esophageal cancer.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Blotting, Western</subject><subject>Carcinoma, Squamous Cell - therapy</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Chemoradiotherapy</subject><subject>DNA Breaks, Double-Stranded</subject><subject>esophageal cancer</subject><subject>Esophageal Neoplasms - therapy</subject><subject>Esophageal Squamous Cell Carcinoma</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Indoles - pharmacology</subject><subject>Pyrroles - pharmacology</subject><subject>Radiation Tolerance - drug effects</subject><subject>Radiation-Sensitizing Agents - pharmacology</subject><subject>radiosensitization</subject><subject>Sunitinib</subject><issn>1120-8694</issn><issn>1442-2050</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UMtOwzAQtBCIlsKFD0C-IHFJsTd24h5RKQ-pUg_0iiwnWVOjJG7jBNS_J33Akb3srGZmNRpCrjkb837uC9_imIMQ7IQMuRAQAZPstMccWKSSiRiQixA-GeNpnKhzMoBECkhjGJL3t652ratdRitfdKVpMdB2hbQxhfMB69CzX67dUm8pBr9emQ80JQ2bzlS-CzTHsqS5aXJX-8rsz0BdTXtP4y_JmTVlwKvjHpHl02w5fYnmi-fX6cM8ymMQLLJQFFJyGUs1sWgmSZxkMlcWrVAgZJbyVKYWAJUSmIFhUmXAQakcFaY2HpG7w9t14zcdhlZXLuySmBr7jJorSBJQgrFeenOUdlmFhV43rjLNVv820gv4QfDtStz-8ZzpXdd617Xed60fF8vZHvWe24PHd-t_HPEP9ax-nQ</recordid><startdate>201611</startdate><enddate>201611</enddate><creator>Ding, Y.-Q.</creator><creator>Zhu, H.-C.</creator><creator>Chen, X.-C.</creator><creator>Sun, X.-C.</creator><creator>Yang, X.</creator><creator>Qin, Q.</creator><creator>Zhang, H.</creator><creator>Yang, Y.</creator><creator>Yang, Y.-H.</creator><creator>Gao, L.</creator><creator>Luo, J.-D.</creator><creator>Zhou, X.-F.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201611</creationdate><title>Sunitinib modulates the radiosensitivity of esophageal squamous cell carcinoma cells in vitro</title><author>Ding, Y.-Q. ; Zhu, H.-C. ; Chen, X.-C. ; Sun, X.-C. ; Yang, X. ; Qin, Q. ; Zhang, H. ; Yang, Y. ; Yang, Y.-H. ; Gao, L. ; Luo, J.-D. ; Zhou, X.-F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3240-f2dd55153589fea9636b5c8fef48245b71757f22e884eb2a058b21288ce8e7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Blotting, Western</topic><topic>Carcinoma, Squamous Cell - therapy</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Chemoradiotherapy</topic><topic>DNA Breaks, Double-Stranded</topic><topic>esophageal cancer</topic><topic>Esophageal Neoplasms - therapy</topic><topic>Esophageal Squamous Cell Carcinoma</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Indoles - pharmacology</topic><topic>Pyrroles - pharmacology</topic><topic>Radiation Tolerance - drug effects</topic><topic>Radiation-Sensitizing Agents - pharmacology</topic><topic>radiosensitization</topic><topic>Sunitinib</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Y.-Q.</creatorcontrib><creatorcontrib>Zhu, H.-C.</creatorcontrib><creatorcontrib>Chen, X.-C.</creatorcontrib><creatorcontrib>Sun, X.-C.</creatorcontrib><creatorcontrib>Yang, X.</creatorcontrib><creatorcontrib>Qin, Q.</creatorcontrib><creatorcontrib>Zhang, H.</creatorcontrib><creatorcontrib>Yang, Y.</creatorcontrib><creatorcontrib>Yang, Y.-H.</creatorcontrib><creatorcontrib>Gao, L.</creatorcontrib><creatorcontrib>Luo, J.-D.</creatorcontrib><creatorcontrib>Zhou, X.-F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Diseases of the esophagus</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Y.-Q.</au><au>Zhu, H.-C.</au><au>Chen, X.-C.</au><au>Sun, X.-C.</au><au>Yang, X.</au><au>Qin, Q.</au><au>Zhang, H.</au><au>Yang, Y.</au><au>Yang, Y.-H.</au><au>Gao, L.</au><au>Luo, J.-D.</au><au>Zhou, X.-F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sunitinib modulates the radiosensitivity of esophageal squamous cell carcinoma cells in vitro</atitle><jtitle>Diseases of the esophagus</jtitle><addtitle>Dis Esophagus</addtitle><date>2016-11</date><risdate>2016</risdate><volume>30</volume><issue>7</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>1120-8694</issn><eissn>1442-2050</eissn><abstract>SUMMARY
This study aims to explore the radiosensitivity of sunitinib on esophageal cancer cell lines. For in vitro studies, human esophageal squamous cell carcinoma (ESCC) cell lines were treated with sunitinib 24 hours before irradiation. ESCC cell lines were treated with sunitinib with or without radiation. Cell proliferation was detected by Cell Counting Kit 8 assay. Radiosensitization was evaluated by clonogenic survival assay. Cell apoptosis and cell cycle analysis were detected by flow cytometry. Deoxyribonucleic acid (DNA) double-strand breaks were performed by immunocytofluorescence analysis. Western blot analysis was used to determine the effect of sunitinib on radiation induced signal transduction. Sunitinib potently sensitized ESCC cells to radiation with a sensitization enhancement ratio of 1.13–1.72. Furthermore, sunitinib increased radiation induced DNA double-strand breaks, promoted the apoptosis of ESCC cells and induced the G2/M arrest. Radiosensitization was accompanied with enhanced apoptosis and regulated by the intrinsic pathway of apoptosis. Sunitinib sensitized ESCC cells to the cytotoxic effects of radiation. This compound is promising for future clinical trials with chemoradiation in esophageal cancer.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>26542732</pmid><doi>10.1111/dote.12440</doi><tpages>8</tpages></addata></record> |
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| subjects | Antineoplastic Agents - pharmacology apoptosis Apoptosis - drug effects Blotting, Western Carcinoma, Squamous Cell - therapy Cell Line, Tumor Cell Proliferation - drug effects Chemoradiotherapy DNA Breaks, Double-Stranded esophageal cancer Esophageal Neoplasms - therapy Esophageal Squamous Cell Carcinoma Flow Cytometry Humans Indoles - pharmacology Pyrroles - pharmacology Radiation Tolerance - drug effects Radiation-Sensitizing Agents - pharmacology radiosensitization Sunitinib |
| title | Sunitinib modulates the radiosensitivity of esophageal squamous cell carcinoma cells in vitro |
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