Identification and characterization of 2-keto-3-deoxygluconate kinase and 2-keto-3-deoxygalactonate kinase in the haloarchaeon Haloferax volcanii

Abstract The halophilic archaeon Haloferax volcanii has been proposed to degrade glucose via the semi-phosphorylative Entner–Doudoroff pathway, involving 2-keto-3-deoxygluconate kinase (KDGK) as key enzyme. So far, neither the enzyme has been characterized nor the encoding gene has been identified....

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Veröffentlicht in:	FEMS microbiology letters 2014-12, Vol.361 (1), p.76-83
Hauptverfasser:	Pickl, Andreas, Johnsen, Ulrike, Archer, Robert M., Schönheit, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Galactose - metabolism Glucose - metabolism glucose degradation Glycolysis Haloarchaea Haloferax volcanii - enzymology Haloferax volcanii - genetics Microbiology Phosphorylation Phosphotransferases (Alcohol Group Acceptor) - genetics Phosphotransferases (Alcohol Group Acceptor) - metabolism semi‐phosphorylative Entner–Doudoroff pathway Sequence Alignment substrate promiscuity
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