Aflatoxin B1-Induced Reproductive Toxicity in Male Rats: Possible Mechanism of Action
Aflatoxin B1 (AFB1), one of the most common mycotoxins found in human foods, is principally hepatotoxic; however, it also affects reproduction. The aim of the present study was to elucidate the reproductive toxic effects and possible mechanism of action of AFB1 in rats. Male Wistar rats were injecte...
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| Veröffentlicht in: | International journal of toxicology 2014-05, Vol.33 (3), p.155-161 |
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| creator | Supriya, Ch Girish, B. P. Reddy, P. Sreenivasula |
| description | Aflatoxin B1 (AFB1), one of the most common mycotoxins found in human foods, is principally hepatotoxic; however, it also affects reproduction. The aim of the present study was to elucidate the reproductive toxic effects and possible mechanism of action of AFB1 in rats. Male Wistar rats were injected intramuscularly with doses of 10, 20, or 50 µg AFB1/kg body weight on alternate days from 45 to 100 days of age. Significant reductions in body weights, relative weights of reproductive organs, daily sperm production, epididymal sperm count, viable sperm, motile sperm, and hypoosmotic swelling-tail coiled sperm were observed. Significant decreases in testicular steroidogenic enzymes and serum testosterone levels were also observed indicating decreased steroidogenesis. In silico docking studies illustrated AFB1 binds with steroidogenic acute regulatory (StAR) protein thereby affecting the transport of cholesterol into mitochondria resulting in decreased steroidogenesis. |
| doi_str_mv | 10.1177/1091581814530764 |
| format | Article |
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P. ; Reddy, P. Sreenivasula</creator><creatorcontrib>Supriya, Ch ; Girish, B. P. ; Reddy, P. Sreenivasula</creatorcontrib><description>Aflatoxin B1 (AFB1), one of the most common mycotoxins found in human foods, is principally hepatotoxic; however, it also affects reproduction. The aim of the present study was to elucidate the reproductive toxic effects and possible mechanism of action of AFB1 in rats. Male Wistar rats were injected intramuscularly with doses of 10, 20, or 50 µg AFB1/kg body weight on alternate days from 45 to 100 days of age. Significant reductions in body weights, relative weights of reproductive organs, daily sperm production, epididymal sperm count, viable sperm, motile sperm, and hypoosmotic swelling-tail coiled sperm were observed. Significant decreases in testicular steroidogenic enzymes and serum testosterone levels were also observed indicating decreased steroidogenesis. In silico docking studies illustrated AFB1 binds with steroidogenic acute regulatory (StAR) protein thereby affecting the transport of cholesterol into mitochondria resulting in decreased steroidogenesis.</description><identifier>ISSN: 1091-5818</identifier><identifier>EISSN: 1092-874X</identifier><identifier>DOI: 10.1177/1091581814530764</identifier><identifier>PMID: 24728861</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Acinetobacter baumannii - enzymology ; Aflatoxin B1 - administration & dosage ; Aflatoxin B1 - chemistry ; Aflatoxin B1 - metabolism ; Aflatoxin B1 - toxicity ; Animals ; Bacterial Proteins - chemistry ; Bacterial Proteins - metabolism ; Binding Sites ; Dose-Response Relationship, Drug ; Epididymis - drug effects ; Epididymis - metabolism ; Epididymis - pathology ; Hydrophobic and Hydrophilic Interactions ; Infertility, Male - chemically induced ; Infertility, Male - metabolism ; Infertility, Male - pathology ; Male ; Models, Molecular ; Molecular Conformation ; Molecular Docking Simulation ; Nucleotidyltransferases - chemistry ; Nucleotidyltransferases - metabolism ; Organ Size - drug effects ; Phosphoproteins - chemistry ; Phosphoproteins - metabolism ; Protein Conformation ; Random Allocation ; Rats, Wistar ; Spermatogenesis - drug effects ; Testis - drug effects ; Testis - metabolism ; Testis - pathology ; Weight Gain - drug effects</subject><ispartof>International journal of toxicology, 2014-05, Vol.33 (3), p.155-161</ispartof><rights>The Author(s) 2014</rights><rights>The Author(s) 2014.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c290t-cb3234b88d7c8336729b7b3d55bdd6b1c15be10e3ef56106904fadcf2eafa4e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1091581814530764$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1091581814530764$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21818,27923,27924,43620,43621</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24728861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Supriya, Ch</creatorcontrib><creatorcontrib>Girish, B. P.</creatorcontrib><creatorcontrib>Reddy, P. Sreenivasula</creatorcontrib><title>Aflatoxin B1-Induced Reproductive Toxicity in Male Rats: Possible Mechanism of Action</title><title>International journal of toxicology</title><addtitle>Int J Toxicol</addtitle><description>Aflatoxin B1 (AFB1), one of the most common mycotoxins found in human foods, is principally hepatotoxic; however, it also affects reproduction. The aim of the present study was to elucidate the reproductive toxic effects and possible mechanism of action of AFB1 in rats. Male Wistar rats were injected intramuscularly with doses of 10, 20, or 50 µg AFB1/kg body weight on alternate days from 45 to 100 days of age. Significant reductions in body weights, relative weights of reproductive organs, daily sperm production, epididymal sperm count, viable sperm, motile sperm, and hypoosmotic swelling-tail coiled sperm were observed. Significant decreases in testicular steroidogenic enzymes and serum testosterone levels were also observed indicating decreased steroidogenesis. In silico docking studies illustrated AFB1 binds with steroidogenic acute regulatory (StAR) protein thereby affecting the transport of cholesterol into mitochondria resulting in decreased steroidogenesis.</description><subject>Acinetobacter baumannii - enzymology</subject><subject>Aflatoxin B1 - administration & dosage</subject><subject>Aflatoxin B1 - chemistry</subject><subject>Aflatoxin B1 - metabolism</subject><subject>Aflatoxin B1 - toxicity</subject><subject>Animals</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - metabolism</subject><subject>Binding Sites</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epididymis - drug effects</subject><subject>Epididymis - metabolism</subject><subject>Epididymis - pathology</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Infertility, Male - chemically induced</subject><subject>Infertility, Male - metabolism</subject><subject>Infertility, Male - pathology</subject><subject>Male</subject><subject>Models, Molecular</subject><subject>Molecular Conformation</subject><subject>Molecular Docking Simulation</subject><subject>Nucleotidyltransferases - chemistry</subject><subject>Nucleotidyltransferases - metabolism</subject><subject>Organ Size - drug effects</subject><subject>Phosphoproteins - chemistry</subject><subject>Phosphoproteins - metabolism</subject><subject>Protein Conformation</subject><subject>Random Allocation</subject><subject>Rats, Wistar</subject><subject>Spermatogenesis - drug effects</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>Weight Gain - drug effects</subject><issn>1091-5818</issn><issn>1092-874X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1Lw0AQxRdRbK3ePUmOXqI7u5vdzbEWv6AilArewn5MJCVtajYR-9-7tdWD4GkeM795zDxCzoFeASh1DTSHTIMGkXGqpDggw9hiqVbi9fBbQ7qdD8hJCAtKqVQZHJMBE4ppLWFI1LisTdd8VqvkBtLHle8d-mSG67aJsqs-MJnHqau6TRKZJ1NjMjNdOCVHpakDnu3riLzc3c4nD-n0-f5xMp6mjuW0S53ljAurtVdOcy4Vy62y3GeZ9V5acJBZBIocy0wClTkVpfGuZGhKI1DwEbnc-caD3nsMXbGsgsO6Nits-lCAZjLLGVd5ROkOdW0TQotlsW6rpWk3BdBiG1fxN664crF37-0S_e_CTz4RSHdAMG9YLJq-XcVv_zf8Aui_cN4</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Supriya, Ch</creator><creator>Girish, B. P.</creator><creator>Reddy, P. Sreenivasula</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140501</creationdate><title>Aflatoxin B1-Induced Reproductive Toxicity in Male Rats</title><author>Supriya, Ch ; Girish, B. P. ; Reddy, P. Sreenivasula</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c290t-cb3234b88d7c8336729b7b3d55bdd6b1c15be10e3ef56106904fadcf2eafa4e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acinetobacter baumannii - enzymology</topic><topic>Aflatoxin B1 - administration & dosage</topic><topic>Aflatoxin B1 - chemistry</topic><topic>Aflatoxin B1 - metabolism</topic><topic>Aflatoxin B1 - toxicity</topic><topic>Animals</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - metabolism</topic><topic>Binding Sites</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epididymis - drug effects</topic><topic>Epididymis - metabolism</topic><topic>Epididymis - pathology</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Infertility, Male - chemically induced</topic><topic>Infertility, Male - metabolism</topic><topic>Infertility, Male - pathology</topic><topic>Male</topic><topic>Models, Molecular</topic><topic>Molecular Conformation</topic><topic>Molecular Docking Simulation</topic><topic>Nucleotidyltransferases - chemistry</topic><topic>Nucleotidyltransferases - metabolism</topic><topic>Organ Size - drug effects</topic><topic>Phosphoproteins - chemistry</topic><topic>Phosphoproteins - metabolism</topic><topic>Protein Conformation</topic><topic>Random Allocation</topic><topic>Rats, Wistar</topic><topic>Spermatogenesis - drug effects</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>Weight Gain - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Supriya, Ch</creatorcontrib><creatorcontrib>Girish, B. P.</creatorcontrib><creatorcontrib>Reddy, P. Sreenivasula</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Supriya, Ch</au><au>Girish, B. P.</au><au>Reddy, P. Sreenivasula</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aflatoxin B1-Induced Reproductive Toxicity in Male Rats: Possible Mechanism of Action</atitle><jtitle>International journal of toxicology</jtitle><addtitle>Int J Toxicol</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>33</volume><issue>3</issue><spage>155</spage><epage>161</epage><pages>155-161</pages><issn>1091-5818</issn><eissn>1092-874X</eissn><abstract>Aflatoxin B1 (AFB1), one of the most common mycotoxins found in human foods, is principally hepatotoxic; however, it also affects reproduction. The aim of the present study was to elucidate the reproductive toxic effects and possible mechanism of action of AFB1 in rats. Male Wistar rats were injected intramuscularly with doses of 10, 20, or 50 µg AFB1/kg body weight on alternate days from 45 to 100 days of age. Significant reductions in body weights, relative weights of reproductive organs, daily sperm production, epididymal sperm count, viable sperm, motile sperm, and hypoosmotic swelling-tail coiled sperm were observed. Significant decreases in testicular steroidogenic enzymes and serum testosterone levels were also observed indicating decreased steroidogenesis. In silico docking studies illustrated AFB1 binds with steroidogenic acute regulatory (StAR) protein thereby affecting the transport of cholesterol into mitochondria resulting in decreased steroidogenesis.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>24728861</pmid><doi>10.1177/1091581814530764</doi><tpages>7</tpages></addata></record> |
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| subjects | Acinetobacter baumannii - enzymology Aflatoxin B1 - administration & dosage Aflatoxin B1 - chemistry Aflatoxin B1 - metabolism Aflatoxin B1 - toxicity Animals Bacterial Proteins - chemistry Bacterial Proteins - metabolism Binding Sites Dose-Response Relationship, Drug Epididymis - drug effects Epididymis - metabolism Epididymis - pathology Hydrophobic and Hydrophilic Interactions Infertility, Male - chemically induced Infertility, Male - metabolism Infertility, Male - pathology Male Models, Molecular Molecular Conformation Molecular Docking Simulation Nucleotidyltransferases - chemistry Nucleotidyltransferases - metabolism Organ Size - drug effects Phosphoproteins - chemistry Phosphoproteins - metabolism Protein Conformation Random Allocation Rats, Wistar Spermatogenesis - drug effects Testis - drug effects Testis - metabolism Testis - pathology Weight Gain - drug effects |
| title | Aflatoxin B1-Induced Reproductive Toxicity in Male Rats: Possible Mechanism of Action |
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