A tightly regulated expression system for E. coli using supersaturated silicic acid

Objective To develop a new expression system regulated by a ferric uptake regulator in which silicic acid is used as an inducer. Results Fur box (binding site for Fur) was substituted for the lac operator to regulate the expression of GFP with the lac promoter. Since the addition of supersaturated s...

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Veröffentlicht in:	Biotechnology letters 2016-08, Vol.38 (8), p.1381-1387
Hauptverfasser:	Fujino, Yasuhiro, Tanoue, Ryo, Yokoyama, Takushi, Doi, Katsumi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Applied Microbiology Bacteriophages Biochemistry Biomedical and Life Sciences Biotechnology E coli Escherichia coli Escherichia coli - metabolism Escherichia coli Proteins - metabolism Gene expression Gene Expression Regulation, Bacterial Genes Inducers Iron Life Sciences Microbiology Nutrient deficiency Original Research Paper Protein expression Regulators Silica Silicic Acid - metabolism Silicon dioxide Uptakes
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