Chromatography-based methods for determining molar extinction coefficients of cytotoxic payload drugs and drug antibody ratios of antibody drug conjugates

•An HPLC method with dual detectors was developed to measure the extinction coefficients.•The MEC was solved by Beer’s law (A=εcl), with A and c obtained from DAD and CLND.•The method measures MEC accurately even when the sample is impure or in a mixture.•Compared to the only available method, our m...

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