Polydimethylsiloxane-Paper Hybrid Lateral Flow Assay for Highly Sensitive Point-of-Care Nucleic Acid Testing

In nucleic acid testing (NAT), gold nanoparticle (AuNP)-based lateral flow assays (LFAs) have received significant attention due to their cost-effectiveness, rapidity, and the ability to produce a simple colorimetric readout. However, the poor sensitivity of AuNP-based LFAs limits its widespread app...

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Veröffentlicht in:Analytical chemistry (Washington) 2016-06, Vol.88 (12), p.6254-6264
Hauptverfasser: Choi, Jane Ru, Liu, Zhi, Hu, Jie, Tang, Ruihua, Gong, Yan, Feng, Shangsheng, Ren, Hui, Wen, Ting, Yang, Hui, Qu, Zhiguo, Pingguan-Murphy, Belinda, Xu, Feng
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container_end_page 6264
container_issue 12
container_start_page 6254
container_title Analytical chemistry (Washington)
container_volume 88
creator Choi, Jane Ru
Liu, Zhi
Hu, Jie
Tang, Ruihua
Gong, Yan
Feng, Shangsheng
Ren, Hui
Wen, Ting
Yang, Hui
Qu, Zhiguo
Pingguan-Murphy, Belinda
Xu, Feng
description In nucleic acid testing (NAT), gold nanoparticle (AuNP)-based lateral flow assays (LFAs) have received significant attention due to their cost-effectiveness, rapidity, and the ability to produce a simple colorimetric readout. However, the poor sensitivity of AuNP-based LFAs limits its widespread applications. Even though various efforts have been made to improve the assay sensitivity, most methods are inappropriate for integration into LFA for sample-to-answer NAT at the point-of-care (POC), usually due to the complicated fabrication processes or incompatible chemicals used. To address this, we propose a novel strategy of integrating a simple fluidic control strategy into LFA. The strategy involves incorporating a piece of paper-based shunt and a polydimethylsiloxane (PDMS) barrier to the strip to achieve optimum fluidic delays for LFA signal enhancement, resulting in 10-fold signal enhancement over unmodified LFA. The phenomena of fluidic delay were also evaluated by mathematical simulation, through which we found the movement of fluid throughout the shunt and the tortuosity effects in the presence of PDMS barrier, which significantly affect the detection sensitivity. To demonstrate the potential of integrating this strategy into a LFA with sample-in-answer-out capability, we further applied this strategy into our prototype sample-to-answer LFA to sensitively detect the Hepatitis B virus (HBV) in clinical blood samples. The proposed strategy offers great potential for highly sensitive detection of various targets for wide application in the near future.
doi_str_mv 10.1021/acs.analchem.6b00195
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To demonstrate the potential of integrating this strategy into a LFA with sample-in-answer-out capability, we further applied this strategy into our prototype sample-to-answer LFA to sensitively detect the Hepatitis B virus (HBV) in clinical blood samples. 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source American Chemical Society Journals
subjects Analytical chemistry
Assaying
Bioassays
Bypasses
Delay
Fluid dynamics
Fluidics
Hepatitis
Hepatitis B virus
Nanoparticles
Nucleic acids
Shunts
Silicone resins
Simulation
Strategy
title Polydimethylsiloxane-Paper Hybrid Lateral Flow Assay for Highly Sensitive Point-of-Care Nucleic Acid Testing
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