Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles
Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium-silicate complex of...
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| Veröffentlicht in: | Analyst (London) 2016-06, Vol.141 (13), p.3982-3984 |
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| container_title | Analyst (London) |
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| creator | Faccio, G Bannwarth, M. B Schulenburg, C Steffen, V Jankowska, D Pohl, M Rossi, R. M Maniura-Weber, K Boesel, L. F Richter, M |
| description | Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium-silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.
Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. |
| doi_str_mv | 10.1039/c5an02573g |
| format | Article |
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F</au><au>Richter, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2016-06-20</date><risdate>2016</risdate><volume>141</volume><issue>13</issue><spage>3982</spage><epage>3984</epage><pages>3982-3984</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium-silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.
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| source | MEDLINE; Royal Society Of Chemistry Journals; Royal Society of Chemistry Journals Archive (1841-2007); Alma/SFX Local Collection |
| subjects | Biosensing Techniques Biosensors Encapsulation Fluorescence Resonance Energy Transfer Fretting Glucose Glucose - analysis Immobilization Maltose Maltose - analysis Nanoparticles Nanoparticles - chemistry Proteins Silicon Dioxide |
| title | Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles |
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