A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand

•Hydrodynamic properties of alginate are modified by papain presence.•Papain neutralizes alginate intra-chain charges and decreases the polymer stiffness.•The alginate-papain interaction is cooperative and enthalpy-entropically driven.•Affinity partitioning allows recovering papain from C. papaya la...

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Veröffentlicht in:	Separation and purification technology 2016-08, Vol.168, p.168-176
Hauptverfasser:	Rocha, Maria Victoria, Di Giacomo, Melisa, Beltramino, Sofía, Loh, Watson, Romanini, Diana, Nerli, Bibiana Beatriz
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Affinity partitioning Alginate Aqueous two-phase systems Biodegradability Carica papaya Enzymes Extraction Latex Papain Papayas Polyethylene glycol Recovering
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