In vitro mesenchymal stem cell response to a CO2 laser modified polymeric material

With an ageing world population it is becoming significantly apparent that there is a need to produce implants and platforms to manipulate stem cell growth on a pharmaceutical scale. This is needed to meet the socio-economic demands of many countries worldwide. This paper details one of the first ev...

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Veröffentlicht in:Materials Science & Engineering C 2016-10, Vol.67, p.727-736
Hauptverfasser: Waugh, D.G., Hussain, I., Lawrence, J., Smith, G.C., Cosgrove, D., Toccaceli, C.
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container_end_page 736
container_issue
container_start_page 727
container_title Materials Science & Engineering C
container_volume 67
creator Waugh, D.G.
Hussain, I.
Lawrence, J.
Smith, G.C.
Cosgrove, D.
Toccaceli, C.
description With an ageing world population it is becoming significantly apparent that there is a need to produce implants and platforms to manipulate stem cell growth on a pharmaceutical scale. This is needed to meet the socio-economic demands of many countries worldwide. This paper details one of the first ever studies in to the manipulation of stem cell growth on CO2 laser surface treated nylon 6,6 highlighting its potential as an inexpensive platform to manipulate stem cell growth on a pharmaceutical scale. Through CO2 laser surface treatment discrete changes to the surfaces were made. That is, the surface roughness of the nylon 6,6 was increased by up to 4.3μm, the contact angle was modulated by up to 5° and the surface oxygen content increased by up to 1atom %. Following mesenchymal stem cell growth on the laser treated samples, it was identified that CO2 laser surface treatment gave rise to an enhanced response with an increase in viable cell count of up to 60,000cells/ml when compared to the as-received sample. The effect of surface parameters modified by the CO2 laser surface treatment on the mesenchymal stem cell response is also discussed along with potential trends that could be identified to govern the mesenchymal stem cell response.
doi_str_mv 10.1016/j.msec.2016.05.055
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source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Adhesion
Caprolactam - analogs & derivatives
Caprolactam - chemistry
Carbon dioxide lasers
Cells, Cultured
CO2 laser
Contact angle
Humans
Lasers, Gas
Materials science
Materials Testing
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Nylons
Pharmaceuticals
Platforms
Polymers - chemistry
Stem cells
Surface engineering
Surface Properties
Surface treatment
Wettability
title In vitro mesenchymal stem cell response to a CO2 laser modified polymeric material
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